Electrochemotherapy to the Bone Lesion of Murine Osteosarcoma

前言电化学疗法是一种新的治疗肿瘤的方法，它可以增强抗肿瘤药物进入肿瘤细胞的能力，在本实验中，我们观察了电化学疗法通过抗肿瘤药物的细胞内转运对小鼠骨肉瘤的疗效。并插入到SCID小鼠后肢的骨髓中。移植后X线片检查肿瘤直径，当肿瘤直径达到5 mm时开始实验，根据奥尔巴赫公式计算肿瘤体积，依次比较药物（D）+电穿孔（E）组和不加药物（D）、不加电穿孔（E）组肿瘤体积的变化。采用生物测定法分析BLM在肿瘤组织中的浓度，并比较D + E组和D + E组的差异。 ...更多信息 结果与结论我们建立了一种小鼠骨肉瘤模型。2个月后肿瘤直径达5mm/mm，X线片示肿瘤占位呈放射性阴影，肿块隆起。在D + E组中，平均肿瘤大小连续减小两个月。然而，在那之后，肿瘤逐渐长大。在A组中：D和E均不作为对照，依次观察到快速的骨破坏和肿瘤生长。一组中的肿瘤组织浓度：D + E，是显着高于另一组：D没有E。我们认为，电穿孔作用于BLM的高积累作为药物运输到骨肿瘤的一种手段。组织学检查显示，6周内肿瘤周围可见较厚的纤维包膜。囊膜内可见巨噬细胞样细胞浸润。肿瘤中心可见肿瘤坏死。肿瘤细胞散在分布于坏死区周围，纤维化程度高。结论：BLM电化学疗法对小鼠骨肉瘤模型的骨损害有较好的治疗作用。少

INTRODUCTIONElectrochemotherapy is a new mode of treatment to enhance the entry of anti-neoplastic agents into tumor cells, In this experiment, we observed the efficacy of electrochemotherapy through intracellular transport of the anti-neoplastic agent on the murine osteosarcoma of bone.MATERIALS AND METHODSTo produce solid murine tumors in bone, resected human osteosarcoma was cut into fine pieces, and inserted into the bone marrow of the posterior limb of SCID mouse. After transplantation, the tumor diameter was checked by X-ray photogram and the experiment was started when it reached 5 mm. Tumor volume was assessed using Auerbach's formula, The changes of tumor volume were compared sequentially among the two groups : Drug(D)+Electroporation(E) and neither D nor E.As an anti-neoplastic agent, we used bleomycin (BLM). The tumor tissue concentration of BLM was analyzed based on the bio-assay and compared among the two groups : D+E and D without E.Histology was investigated on the pseud … More ocapsules and tumor necrosis.RESULTS AND DISCUSSIONSWe made a murine osteosarcoma model which had a bone lesion. It took two months till the tumor size reached 5 mm in a dimeter, And the tumor occupying lesion was seen as a radiolucent and bulging area by the X-ray photogram. In a group : D+E, mean tumor size was lessened sequentially for two months. However, the tumor was gradually grown after that. In a group : neither D nor E as a control, rapid bone destruction and tumor growth were sequentially observed. The tumor tissue concentration in a group : D+E, was significantly higher than that of the another group : D without E.We believe that electroporation acted on an high accumulation of BLM as a mean of the drug transport to the bone tumor. Histological investigation revealed that the thick fibrouscapsule was observed around the tumor in six weeks. And there were cell infiltrations of macrophage-like cells in this capsule. Tumor necrosis was observed in the cener of the tumor. Tumor cells were sporadic surrounding the necrotic area and the fibrosis was dense in these area. We concluded that electrochemotherapy wiith BLM acted on an effective mode to the bone lesion of murine osteosarcoma model. Less