The roles of ret/PTC fusion genes in thyroid papillary carcinoma

ret/PTC融合基因在甲状腺乳头状癌中的作用

• 批准号: 11671671
• 负责人: NIBU Ken-ichi
• 金额: $ 1.73万
• 依托单位: Kobe University Graduate School of Medicine (2001)The University of Tokyo (1999-2000)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671671/
• 关键词: Thyroid; papillary carcinoma; ret; p53; papillary carcinoma; ras; PTC; thyroid; papillary; carcinoma

Background: The activation of ret proto-oncogene, through chromosomal translocation, is reported as unique to papillary thyroid carcinomas. However, the prevalence of RET/PTC activation in papillary carcinoma reported was variable and the clinical relevance of RET/PTC rearrangements in papillary carcinomas is still controversial.Methods: To investigate the roles of ret rearrangement in the carcinogenesis of papillary thyroid carcinoma, we have studied ret activation and p53 overexpression in various thyroid lesions of Japanese population by immunohistochemical technique.Results: Ret activation and p53 overexpression were studied in 40 papillary carcinomas, 5 poorly differentiated carcinomas, 1 undifferentiated carcinoma, 2 medullary carcinoma, 2 follicular carcinomas, 19 follicular adenomas. RET activation was observed in 12 out of 40 papillary carcinomas, while no immunoreactivity of ret was detected in other lesions. P53 overexpression was observed in only one of 40 papillary carcinomas.Conclusion: The prevalence of RET/PTC activation in papillary carcinoma among Japanese population was higher than previous reports. Immunohistochemistry is proved as a useful tool to detect RET/PTC activation in thyroid tumors. RET rearrangements are restricted to a well-differentiated papillary carcinoma, suggesting that RET/PTC positive papillary carcinomas do not progress to undifferentiated carcinoma.

背景：ret原癌基因的激活，通过染色体易位，报道是独特的乳头状甲状腺癌。然而，报道的乳头状癌中RET/PTC激活的患病率是可变的，RET/PTC重排在乳头状癌中的临床相关性仍然存在争议。方法：采用免疫组化技术研究日本人群甲状腺病变中ret的活化和p53的过表达，探讨ret重排在甲状腺乳头状癌发生中的作用。结果：在40例乳头状癌、5例低分化癌、1例未分化癌、2例髓样癌、2例滤泡癌、19例滤泡腺瘤中检测到Ret激活和p53过表达。40例乳头状癌中有12例观察到RET激活，而在其他病变中未检测到RET的免疫反应性。40例乳头状癌中仅有1例P53过表达。结论：日本人乳头状癌中RET/PTC激活的发生率高于以往的报道。免疫组织化学被证明是检测甲状腺肿瘤中RET/PTC激活的有效工具。RET重排仅限于分化良好的乳头状癌，提示RET/PTC阳性乳头状癌不会发展为未分化癌。

项目成果

Yokoo S, et al:: "Indications for vascularized free rectus abdominis musculocutaneous flap in oromandibular region in terms of efficacy of anterior rectus sheath"Microsurgery. 23. 96-102 (2003)

Yokoo S 等人：“口下颌区血管化游离腹直肌肌皮瓣在前直肌鞘功效方面的适应症”显微外科。

Yokoo S, et al.: "Indications for vascularized free rectus abdominis musculocutaneous flap in oromandibular region in terms of efficacy of anterior rectus sheath"Microsurgery. 23. 96-102 (2003)

Yokoo S 等人：“口下颌区血管化游离腹直肌肌皮瓣在前直肌鞘功效方面的适应症”显微外科。

Ishibashi T: "Identification of dual specificity phosphatases induced by olfactory bulbectomy in rat olfactory neuroepithelium"Brain Research. 902. 205-201 (2001)

Ishibashi T：“大鼠嗅神经上皮嗅球切除术诱导的双特异性磷酸酶的鉴定”脑研究。

Toma M: "Partial laryngectomy to treat early glottic cancer after failure of radiation therapy"Arch Otolaryngol Head Neck Surg. 128. 909-912 (2002)

Toma M：“部分喉切除术治疗放射治疗失败后的早期声门癌”Arch Otolaryngol Head Neck Surg。

Ishida H: "Invasion of the skull base by carcinomas : histopathologically evidenced findings with CT and MRI"Eur Arch Otorhinolaryngol. 259. 535-539 (2002)

Ishida H：“癌对颅底的侵袭：CT 和 MRI 的组织病理学证据”Eur Arch Otorhinolaryngol。