Local IgE synthesis in allergic rhinitis and effect of DEP on upregulating allergic inflammation

变应性鼻炎患者的鼻肥大细胞（NMC）表现出FcεRI表达增加，TL-4和IgE可上调肥大细胞中FcεRI的表达（J Clin Invest 1997，Clin exp Allergy 1998）。这种增加的FcεRI在NMC中的表达与IgE结合性的增加以及介质释放的增加相关。此外，PBMC中FcεRI的表达与血清IgE水平呈正相关。肥大细胞还能诱导B细胞合成IgE。γ δ T细胞也增加变应性鼻炎，他们释放更高水平的IL-4和IL-13比α β T cells.在这里，我们首先分析了局部IgE合成过敏性鼻炎患者的鼻粘膜后，体外刺激Derf II。(mite抗原）。然后，我们通过测量单独用Derf II或Derf II与DEP组合刺激后培养上清液中的水平，分析DEP对肥大细胞和γ δ T细胞产生细胞因子（IL-4、IL-5、IL-6和IL-13）的影响。IL-4和IL-13 mRNA也上调。DEP可使鼻肥大细胞和γ δ T细胞分泌更多的细胞因子，提示在变应性炎症部位存在局部IgE合成，提示NMC通过诱导局部IgE合成在调节慢性变应性炎症中起重要作用。DEP通过其对肥大细胞和γ δ T细胞的作用在增强过敏性炎症中具有关键作用。

Nasal mast cells(NMC) in allergic rhinitis patients exhibit increased expression of the FcεRI, and TL-4 as well as IgE can upregulate the FcεRI expression in mast cells (J Clin Invest 1997,Clin exp Allergy 1998). This enhanced expression of the FcεRI in NMC was associated with an increase in IgE-bindability as well as increased mediator release. Furthermore, the FcεRI expression in NMC correlated well with the levels of serum IgE.. Moreover mast cells can induce IgE synthesis in B cedlls. Gamma delta T cells are also increased in allergic rhinitis and they release higher levels of IL-4 and IL-13 than alpha beta T cells.Here we first analyzed the local IgE synthesis in the nasal mucosa of patients with alletgic rhinitis after invitro stimulation with Derf II. (mite antigen) by RT-PCR. We then analyzed the effect of DEP on cytokine production (IL-4,IL-5,IL-6 and IL-13) from mast cells and gamma delta T cells by measuring the levels in the culture supernatants after stu=imulating with DerfII alone or DerfII in combination wit DEP.An upregulation of the c epsilon mRNA was detected in vitro after stimulating with Derf II. IL-4 and IL-13 mRNA were also upregulated. DEP produced more enhanced cytokine secretion from nasal mast cells as well as gamma delta T cells.These findings suggest that local IgE synthesis occurs at sites of allergic inflammation and indicates a very important role for NMC in regulating chronic allergic inflammation by inducing local IgE synthesis. DEP has a key role in enhancing allergic inflammation by its effect on mast cells and gamma delta T cells also.