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Novel Roles of mast cells in Eosinophilic nasal polyps including aspirin sensitive nasal polyps

肥大细胞在嗜酸性鼻息肉（包括阿司匹林敏感鼻息肉）中的新作用

基本信息

批准号：
18591889
负责人：
PAWANKAR Ruby
金额：
$ 2.43万
依托单位：
Nippon Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591889/
关键词：
Nasal Polyps Mast cell MC(T) and MC(TC)FcεRI Eosinophils MMP-9 IL-13 IgE AIA IL-4,IL-13 AIAに伴う鼻茸 TLR

项目摘要

The mechanisms of eosinophilic nasal polyps and aspirin induced nasal polyps is not well defined. Most studies have focused on investigating the epithelial cells and eosinophilic mediators. Mast cells are increased in the nasal polyps and are important inflammatory cells and are particularly increased in eosinophilic nasal polyps and aspirin sensitive nasal polyps and may play a role in the infiltration of eosinophilsIn the present study, we used the the mast cell culture system we established to investigate the activating mechanisms of the nasal polyp mast cells. Upon stimulating nasal polyp mast cells with the FceRI antibody we investigated the levels of histamine, tryptase, IL-4, IL-5 TNF-alpha, IL-13 and TGF-beta by ELISA. Histamine, tryptase, IL-, IL-5, IL-13, TNF-alpha were increased in the cell supernatants of stimulated mast cells.The levels of histamine and eosinophilic cationic protein that are proteins of mast cells and eosinophils respectively gad a good correlation. From the point of view of increased IL-4 and IL-13, these are related to the increased levels of IgE in nasal polyps. Histaimine from nasal polyp mast cells upregulates RANTES production from nasal epithelial cells and this in turn can induce increased eosinophil infiltration into the nasal polypMMP-9 also was increased in nasal polyp. TIMP-1 and TIMP-2 were less. Mast cells and eosinophils exprrssed MMP-9. This can induce the structural changes seen in eosinophilic nasal polyps

嗜酸性粒细胞性鼻息肉和阿司匹林诱发鼻息肉的机制尚不清楚。大多数研究集中在研究上皮细胞和嗜酸性介质。肥大细胞在鼻息肉中大量存在，是重要的炎性细胞，在嗜酸性粒细胞性鼻息肉和阿司匹林敏感性鼻息肉中尤为明显，并可能在嗜酸性粒细胞浸润中发挥作用。在用FceRI抗体刺激鼻息肉肥大细胞后，我们通过ELISA研究了组胺、类胰蛋白酶、IL-4、IL-5、TNF-α、IL-13和TGF-β的水平。肥大细胞培养上清中组胺、类胰蛋白酶、IL-1、IL-5、IL-13、TNF-α水平升高，肥大细胞和嗜酸性粒细胞的组胺和嗜酸性粒细胞阳离子蛋白水平呈良好的相关性。从IL-4和IL-13升高的角度来看，这些都与鼻息肉中IgE水平升高有关。来自鼻息肉肥大细胞的组胺上调鼻上皮细胞的RANTES产生，这反过来可以诱导嗜酸性粒细胞浸润到鼻息肉中增加，MMP-9也在鼻息肉中增加。MMP-9在肥大细胞和嗜酸性粒细胞中表达。这可以引起嗜酸性鼻息肉中所见的结构变化