Role of mast cells in allergic rhinitis and nasal polyps : Gene chip Analysis

肥大细胞在过敏性鼻炎和鼻息肉中的作用：基因芯片分析

• 批准号: 15591835
• 负责人: PAWANKAR Ruby
• 金额: $ 2.18万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591835/
• 关键词: Allergic Rhinitis; Nasal Polyp; Mast Cell; Gene Chip; 鼻茸; MC(T)とMC(TC); FcεRI; TLR

The objective of our research was to elucidate the role of mast cells in nasal allergy as well as nasal polyps, Firstly, we isolated CD34+progenitor cells from nasal turbinates of allergic rhinitis patients, and nasal polyps including those with aspirin sensitivity. Then we cultured these cells to isolate MC and MCTC mast cells. We then stimulated these mast cells via the FcεRI and TLR. We found that MCTC mast cells express CCR3 and CCR5 and express GMCSF, IL-4,IL-5,IL-6,IL-8,IL-13,IL=16 and eotaxin. When these cells were stimulated via TLR MCTC cells express GMCSF, IL-4,IL-5,IL-6,IL-8,IL-10,IL-13,IL-16 and RANTES.It is known that there is an increase in mast cells in the nasal epithelium of allergic rhinitics. Using this cultured mast cell model, we found that RANTES is an important chemotactic factor for mast cell migration into the epithelium. We also found that Stem cell factor (SCF) upregulates chymase expression in mast cells. Furthermore, we FACS sorted Cd34+ progenitor cells and cultured them with SCF IL-6 in 96well plate in methyl cellulose culture to form a colony culture. Then from 10^7 calls we extractedmRNA to further analyze their characteristics by Gene chip analysis

本研究的目的是为了阐明肥大细胞在鼻变态反应和鼻息肉中的作用。首先，我们从变应性鼻炎患者的鼻甲和包括阿司匹林敏感者在内的鼻息肉中分离出CD34+祖细胞。然后对这些细胞进行培养，分离MC和MCTC肥大细胞。然后，我们通过Fc、ε、RI和TLR刺激这些肥大细胞。我们发现MCTC肥大细胞表达CCR3和CCR5，表达GMCSF、IL-4、IL-5、IL-6、IL-8、IL-13、IL=16和嗜酸性粒细胞趋化因子。经TLR刺激后，MCTC细胞表达GMCSF、IL-4、IL-5、IL-6、IL-8、IL-10、IL-13、IL-16和RANTES。利用这个培养的肥大细胞模型，我们发现RANTES是肥大细胞迁移到上皮细胞的重要趋化因子。我们还发现，干细胞因子(SCF)上调肥大细胞中糜酶的表达。进一步采用流式细胞仪对CD34+祖细胞进行分选，并在96孔板中加入干细胞因子IL-6，在甲基纤维素板上进行集落培养。然后从10^7个呼叫中提取mRNA，通过基因芯片分析进一步分析它们的特征

项目成果

Mast cells in allergic airway disease and chronic rhinosinusitis

• DOI: 10.1159/000087639
• 发表时间: 2005-01-01
• 期刊: MAST CELLS IN ALLERGIC DISEASES
• 作者: Pawankar, R

IFN and IL-4 regulate the expression of CC-chemokines(RANTES,MCP-4,TARC)by TLR ligands stimulated nasal fibroblasts

IFN和IL-4通过TLR配体刺激鼻成纤维细胞调节CC趋化因子(RANTES、MCP-4、TARC)的表达

• 发表时间: 2005
• 期刊: J Allergy Clinical Immunol 115(2)1
• 作者: Nonaka M;Ogihara N;Pawankar R;Fukumato A;Sakanusyi A;Yagi T.
• 通讯作者: Yagi T.

Pathophysiology of Rhinosinusitis

鼻窦炎的病理生理学

• 发表时间: 2005
• 作者: Pawankar R;et al.
• 通讯作者: et al.

RANTES but not SCF induces mast cell migration into the allergic nasal epithelium.

RANTES（而非 SCF）诱导肥大细胞迁移至过敏性鼻上皮。

• 期刊: (In press)
• 作者: Pawankar R;Yamagishi S;Takizawa R;Ozu C.
• 通讯作者: Ozu C.

Pawankar R et al.: "Pathophysiological Mechanisms of Chronic Rhinosinusitis"Immunol Allergy Clin North America. 24. 75-85 (2004)

Pawankar R 等人：“慢性鼻窦炎的病理生理机制”北美免疫过敏临床。