Mechanisms of mast cell migration into the allergic nasal epithelium.

肥大细胞迁移到过敏性鼻上皮的机制。

• 批准号: 13671807
• 负责人: PAWANKAR Ruby
• 金额: $ 2.18万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671807/
• 关键词: Nasal Allergy; Mast cell; MC(T) and MC(TC); RANTES; Stem Cell Factor; Mast cells; MC(T); Allergy; MC(TC); Nasal allergy; Mast cell; MC(TC), MC(T); Stem Cell Factor

Mast cells are increased in the nasal epithelium of patients with allergic rhinitis (AR). Yet, the precise mechanism is unclear. SCF is considered to be an important chemotactic factor for mast cells. Here we examined the levels of SCF, and other chemokines like RANTES, eotaxin, IL-8 in nasal epithelial layer and deep lamina propria. We also examined the levels released by cultured nasal epithelial cells (CNEC) and fibroblasts. We then analyzed the chemotactic activity of each of these chemokines for mast cells.By ELISA, we examined the levels of RANTES, eotaxin and SCF in homogenized nasal scrapings and LP of AR patients as well as CNEC and fibroblast culture supernatants. We studied the chemotactic activity by chemotaxis assay.The levels of RANTES, but not Eotaxin and SCF was greater in the epithelium than in the LP. Moreover CNEC produced higher levels of RANTES but fibroblasts produced higher levels of eotaxin, SCF and IL-8. At the 1 hr time point, RANTES induced more profound chemotasis of mast cells as compared to other chemokines and SCF.These results strongly suggest that RANTES is a critical factor regulating mast cell migration into the allergic nasal epithelium.

肥大细胞在变应性鼻炎（AR）患者的鼻上皮中增加。然而，确切的机制尚不清楚。SCF被认为是肥大细胞的重要趋化因子。我们检测了SCF和其他趋化因子如RANTES、嗜酸性粒细胞趋化因子、IL-8在鼻上皮层和深层固有层中的水平。我们还检测了培养的鼻上皮细胞（CNEC）和成纤维细胞释放的水平。然后我们分析了这些趋化因子对肥大细胞的趋化活性。通过ELISA，我们检测了AR患者的鼻刮片和LP以及CNEC和成纤维细胞培养上清液中RANTES，eotaxin和SCF的水平。趋化性实验表明，RANTES在上皮细胞中的表达高于LP，而Eotaxin和SCF在上皮细胞中的表达低于LP。此外，CNEC产生更高水平的RANTES，但成纤维细胞产生更高水平的嗜酸性粒细胞趋化因子、SCF和IL-8。在1小时的时间点，RANTES诱导更深刻的趋化性肥大细胞相比，其他趋化因子和SCF。这些结果强烈表明，RANTES是一个关键因素调节肥大细胞迁移到过敏性鼻上皮。

项目成果

Novel concepts in the pathogenesis of nasal polyps

鼻息肉发病机制的新概念

• 期刊: Proceedings IFOS consensus meeting 2001 In press
• 作者: Pawankar R;Yamagishi S
• 通讯作者: Yamagishi S

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