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ROLES OF gammadelta TCELLS AND MAST CELLS IN ALLERGIC RHINITIS

γδ TC细胞和肥大细胞在过敏性鼻炎中的作用

基本信息

批准号：
09671766
负责人：
PAWANKAR Ruby
金额：
$ 1.86万
依托单位：
NIPPON MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671766/
关键词：
Mast cells IL-4 IgE gammadelta T cell CD4+ nasal gammadelta T cell perennial allergic rhinitis FcεRI アレルギー性鼻炎 γδT細胞 Th2型サイトカイン粘膜免疫 CD4+,CD4-8-γδT細胞

项目摘要

* Nasal gammadelta T cell clones from PAR patients were predominantly CD4-8- and 0D4+and these clones were antigen specific. CD4_ nasal gammadelta T cell clones predominantly expressed the Vgammal/Vdelta-JM gene. CD4+ nasal gammadelta T cell clones expressed increased levels of IL-4, IL-S and IL-13, but negligible IFN-gamma. By contrast, peripheral blood gd T cell clones were 0D8+ and CD4-8-, not antigen specific, Vgamma2fVdelta2 + and expressed increased levels of IFN-gamma.* NMC from both PAR and CIR patients expressed a variety of cytokines, but significant differences were observed in the proportion of cytokine expressing cells between PAR and CIR in that NMC from PAR patients expressed high levels of IL-4, IL-5, IL-6 and IL-13. Also NMC from PAR patients exhibited increased FcERI expression, cell-bound IgE and IgE-mediated histamine and cytokine release as compared to NMC from CIR patients, even after saturation of the IgE receptors. The density of IgE receptors and IgE molecules in NMC of PAR patients correlated well with the levels of serum igE, and I L-4 upregulated the expression of the FceRl in NMC.Moreover, NMC from PAR patients induced IgE synthesis in B cells. Steroids inhibited the synthesis of IL-4 and 11-13 from nasal mast cells. Taken together, these results and the recently demonstrated IgE-induced upregutation of the FcuRl expression in mast cells suggest critical roles for mast cells in promoting the allergic reaction through an lgE-FcERI-mast cell axis.

* 来自PAR患者的鼻γ δ T细胞克隆主要是CD 4 -8-和CD 4+，并且这些克隆是抗原特异性的。CD 4_鼻γ δ T细胞克隆主要表达Vgammal/Vdelta-JM基因。CD 4+鼻γ δ T细胞克隆表达增加的IL-4、IL-S和IL-13水平，但IFN-γ可忽略不计。相比之下，外周血gd T细胞克隆是CD 8+和CD 4 -8-，不是抗原特异性的，V γ 2fVdelta 2+，并且表达增加水平的IFN-γ。PAR和CIR患者的NMC均表达多种细胞因子，但在PAR和CIR之间的细胞因子表达细胞比例中观察到显著差异，因为PAR患者的NMC表达高水平的IL-4、IL-5、IL-6和IL-13。与CIR患者的NMC相比，PAR患者的NMC也表现出增加的FcERI表达、细胞结合IgE和IgE介导的组胺和细胞因子释放，即使在IgE受体饱和后。PAR患者PBMC中IgE受体和IgE分子的密度与血清IgE水平相关，IL-4可上调PBMC中FceR 1的表达，并诱导B细胞合成IgE。类固醇抑制鼻肥大细胞合成IL-4和11-13。总之，这些结果和最近证实的肥大细胞中IgE诱导的FcuRl表达上调表明肥大细胞在通过IgE-FcERI-肥大细胞轴促进过敏反应中的关键作用。