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The roles of leukocytes during the thrombus formation in the microcirculation of skin

白细胞在皮肤微循环血栓形成过程中的作用

基本信息

批准号：
11671782
负责人：
NAKANISHI Hideki
金额：
$ 2.18万
依托单位：
The Univ.of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671782/
关键词：
rabbit ear chamber microcirculation of skin thrombus formation leukocyte superoxide hydroxyl radical

项目摘要

We have studied about thrombus formation in the microcirculation of the skin. And we have found that the morphological composition of thrombi in the arterioles and venules was different.A transparent round-table chamber (rabbit ear chamber : REC) was implanted in the earlobe of Japanese white rabbits. A complete microvascular network had formed in the chamber, and arterioles 20-30μm in diameter and venules 45-55μm in diameter were selected for experiments. Filtered light (400-500 nm light passed through a fluorescein isothiocyanate excitation filter from a high pressure mercury lamp) was irradiated onto the vessels through an objective lens. Thrombus in the microvessels was formed by the filtered light and solution of fluorescein sodium in the vessels. In the arterioles, the thrombi consisted of platelet aggregation, but in the venules, platelets and leukocytes accumulated on the endothelium.We investigated the effects of the iron chelator desferrioxamine (DFX) on thrombus formation in the arterioles and venules of rabbit ear chamber. Occlusive thrombus formation time (o-time) was significantly extended by DFX both in the arterioles and the venules. This suggests that hydroxyl radicals may be important mediators in this model, as DFX is known as a hydroxyl radical scavenger.On the study of the administration of the CD-18 and anti L-selectin antibody, the o-time was prolonged in the venules in which many leukocytes was observed on the thrombus formation.We also assessed the endothelial dysfunction od microvessels by measuring viscosity of leukocytes in venules and the diameters of arterioles. In alloxan-induced diabetic rabbits, the adherent time of leukocytes to endothelium of venules was longer than in control rabbits. The results of the study of the administration of acetylcholine, L-arginine and sodium nitroprusside suggested the impairment of microvasculat relaxation in hyperglycemic rabbits.

我们研究了皮肤微循环中血栓的形成。在日本白色家兔耳垂内植入透明的圆桌状腔（兔耳腔）。实验选用直径为20-30μm的小动脉和直径为45-55μm的小静脉。过滤光（来自高压汞灯的400-500 nm光通过异硫氰酸荧光素激发滤光器）通过物镜透镜照射到容器上。微血管内血栓是由滤过光和荧光素钠溶液在血管内形成的。在家兔耳腔微动脉血栓形成中，血小板聚集，而在微静脉血栓形成中，血小板和白细胞聚集在内皮细胞上，本文观察了去铁胺（DFX）对兔耳腔微动脉和微静脉血栓形成的影响。DFX显著延长了小动脉和小静脉的闭塞血栓形成时间（o-时间）。这表明羟基自由基可能是该模型中的重要介质，因为DFX被称为羟基自由基清除剂。o-微静脉内血栓形成时间延长，白细胞增多，通过测定微静脉内白细胞粘度和微血管直径，评价微血管内皮功能障碍小动脉四氧嘧啶糖尿病兔白细胞粘附于微静脉内皮细胞的时间较对照组延长。乙酰胆碱、L-精氨酸和硝普钠对高血糖家兔微血管舒张功能的影响。