Versatile Study about Skin Microcirculatory Failures on Rabbit Diabetes Mellitus Model

兔糖尿病模型皮肤微循环障碍的综合研究

• 批准号: 13671880
• 负责人: NAKANISHI Hideki
• 金额: $ 2.24万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671880/
• 关键词: Diabetes mellitus; Microcirculation of skin; Flap necrosis; Thrombus formation; Rabbit ear chamber

(1) Study in the rabbit ear chamberWe can observe arterioles, capillaries, and venules in the rabbit ear chamber, and can also observe red and white blood cell and platelet in the each vessel. The chamber was installed on ears of the Japanese white rabbits which were diabetes mellitus. In the diabetes mellitus models, rolling of white blood cells was increased in the venules of the chamber. In the study about the reaction of the vessel diameter to vasodilator drugs, disfunction of the vascular dilatation was observedIn the study about sarpogrelate, rolling of white blood cells in the venules was decreased in the treated group. We made artificial thrombus in the microvessels of the chamber in the diabetes mellitus model. Thrombus formation time in the arterioles and the venules was prolonged in the drug treated, group. The main component of the thrombus in the arterioles was platelets. The main components in the venules were white and red blood cells and platelets. We think the white blood cells was important on the venules because sticking of these cells were initially observed on the vessels(2) Study about flap necrosisDistal necrosis was observed on the random pattern flap on the back of the diabetes mellitus rabbits. Global necrosis was observed on the island flap on the abdomen. Macroscopically, necrosis area of the flaps was increased in the both groups. Microscopically, thrombus formation in the deep vessels was observed in the both groups

(1)兔耳腔研究我们可以观察到兔耳腔内的小动脉、毛细血管、小静脉，也可以观察到各血管内的红细胞、白细胞和血小板。在糖尿病的日本大白兔耳上安装腔室。在糖尿病模型中，心室小静脉中白细胞滚动增加。在血管扩张药物对血管直径反应的研究中，观察到血管扩张功能障碍。在沙丁鱼酸盐的研究中，治疗组小静脉中白细胞滚动量减少。我们在糖尿病模型的心室微血管中制造人工血栓。药物治疗组小动脉和小静脉血栓形成时间明显延长。小动脉血栓的主要成分是血小板。小静脉的主要成分是白细胞、红细胞和血小板。我们认为白细胞在小静脉上的作用是重要的，因为这些细胞最初在血管上粘附。(2)皮瓣坏死的研究在糖尿病兔背部的随机皮瓣上观察到远端坏死。腹部岛状皮瓣全身性坏死。宏观上看，两组皮瓣坏死面积均增加。镜下两组均可见深血管血栓形成

项目成果

Ichiro Hashimoto: "The Features of Thrombus in a Microvessel Injury Model and the Antithrombotic Efficacy of Heparin, Urokinase, and Prostaglandin E1"Plastic and Reconstructive Surgery. 111・7. 2307-2314 (2003)

桥本一郎：“微血管损伤模型中的血栓特征以及肝素、尿激酶和前列腺素 E1 的抗血栓功效”整形外科 111・7（2003 年）。

Ichiro Hashimoto: "The Gluteal Fold Flap for Vulvar and Buttock Reconstruction : Anatomical Study and Adjustment of Flap Volume"Plastic and Reconstructive Surgery. 108-7. 1998-2005 (2001)

桥本一郎：“用于外阴和臀部重建的臀皱皮瓣：解剖学研究和皮瓣体积的调整”整形外科。

Ichiro.Hashimoto: "Angiostatic effects of corticosteroid on wound healing of the rabbit ear"The Journal of Medical Investigation. Vol.49, No.1,2. 61-66 (2002)

Ichiro.Hashimoto：“皮质类固醇对兔耳伤口愈合的血管抑制作用”医学调查杂志。

Ichiro Hashimoto: "Transcutaneous PO2 and PCO2 Monitoring for Flap Transplanted in Microsurgery"Proceedings of the Inaugural Congress of the World Society for Reconstructive Microsurgery, Monduzzi Editore, Italy. 21-22 (2001)

Ichiro Hashimoto：“显微外科皮瓣移植的经皮 PO2 和 PCO2 监测”世界重建显微外科学会首届大会论文集，意大利 Monduzzi Editore。

Ichiro Hashimoto: "Angiostatic effects of corticosteroid on wound healing of the rabbit ear"The Journal of Medical Investigation. 49・1-2. 61-66 (2002)

桥本一郎：“皮质类固醇对兔耳伤口愈合的血管抑制作用”《医学调查杂志》49・1-66（2002）。