权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Study of the skin microcirculation in the rabbit ear suffering from hyperlipidemia or hypergricemia

高脂血症、高血糖兔耳皮肤微循环的研究

基本信息

批准号：
16390510
负责人：
NAKANISHI Hideki
金额：
$ 9.18万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2007
项目状态：
已结题

项目摘要

The aim of this study is investigations of relation between disturbances of skin microcirculation and hyperlipidemia or hypergricemia. This study would help the development of clinical treatments in the skin circulation disorder after flap transplantation surgery. Effects of hyperlipidemia or hypergricemia to the microcirculation of flap are not clear at the moment. The characteristics of these studies in our laboratory am using rabbit ear chamber as a model of skin microcirculation and observing dynamic course of thrombosis formation in the rabbit ear chamber. The blood circulation of flaps in this study can be observed using transcutaneous PO2 (TcPO2), transcutaneous PCO2 (TcPCO2) and laser Doppler flow meter.The effect of improvement in the survival rate on the skin flap was observed after administration of inhibitor of the neutrophil elastase. The neutrophil invasion to the skin de/this was suppressed using the neutrophil elastage. This drug also suppresses thrombus formation in injured microvasculature of rabbits Different effects o f the suppression of thrombus formation was observed in the arterioles and venules.Sarpogrelate hydrochloride, an antagonist of 5-hydroxytryptamine receptor improved the survival rate of skin flaps elevated on the back of rabbits and suppressed thrombus formation in injured microvessels of rabbits. Different effects o f the suppression of thrombus formation was observed in the arterioles and venules.The developing rate of the microcirculation in the hyperlipidemia rabbit was almost same as the rate in normal rabbits. The reasons of this result were not revealed in this study. Following study has to be performed in the hyperlipidemia rabbit.

本研究旨在探讨皮肤微循环障碍与高脂血症或高血糖的关系。本研究将有助于临床治疗皮瓣移植术后皮肤循环障碍。高脂血症或高血糖对皮瓣微循环的影响目前尚不清楚。本实验室这些研究的特点是用兔耳腔作为皮肤微循环的模型，观察兔耳腔血栓形成的动态过程。应用经皮血氧饱和度(TcPO2)、经皮血二氧化碳分压(TcPCO2)和激光多普勒血流仪观察皮瓣血循环，观察应用中性粒细胞弹性酶抑制剂后对皮瓣成活率的影响。使用中性粒细胞弹性体可抑制中性粒细胞对皮肤的侵袭。该药还可抑制兔损伤微血管内血栓的形成，对微动脉和静脉内血栓形成有不同程度的抑制作用。5-羟色胺受体拮抗剂盐酸沙波格雷可提高兔背部升高的皮瓣存活率，抑制兔损伤微血管内血栓的形成。对微动脉和静脉血栓形成有不同程度的抑制作用。高脂血症兔微循环发育速度与正常兔基本相同。这一结果的原因在这项研究中没有被揭示。后续研究还需在高脂血症兔身上进行。