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Monitoring of oxygen-derived radicals : development and appplication of optical biosensor to oral tissues under pathological conditions

氧自由基的监测：病理条件下口腔组织光学生物传感器的开发和应用

基本信息

批准号：
11671858
负责人：
TODOKI Kazuo
金额：
$ 2.3万
依托单位：
Kanagawa Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

Reactive hyperemia, the local vasodilatation which occurs in response to oxygen debt and accumulation of metabolic waste products due to interruption of blood flow ; active hyperemia, and the increased blood flow in an organ during a period of activity. The microcirculation is controlled partly by sympathetic nerve and partly by vasoactive substances secreted locally by the endothelial cells. The most important of the latter is nitric oxide(NO), which facilitates flow by causing relaxation of vascular smooth muscle. In the present study, we investigated the role of NO in the regulation of local circulation in gingival tissue during reactive hyperemia, using laser Doppler flowmetry and NO electrochemical technique.METHODS : The experiments were performed in beagle dogs under anesthesia by sodium pentobarbital (25 mg/kg, i.v.). Gingival blood flow (GBF) was measured in attached gingival tissue of right mandibular canine tooth using a laser Doppler flowmeter. Reactive hyperemia was elicit … More ed by release of occlusive compression (30, 60 and 300 sec period), which laser Doppler flowmeter probe was applied against to the gingival tissue at a force that caused less than GBF 2 ml/min/100 g. In order to perform pharmacological consideration of reactive hyperemia generated in gingival tissue, intra-arterial bolus injection of various drugs were given.RESULTS : The GBF increased depending on occlusion durations of 30, 60, and 300 sec by compressing of gingival tissue. The reactive hyperemia was significantly suppressed by pretreatment with N ω-nitro-L-arginine methyl ester (L-NAME, 60 mg/kg, i.a.), an inhibitor of NO synthase, but not by atropine (muscarinic antagonist), propranolol(β-blocker), pilylamine (H1-blocker), cimetidine (H2-blocker). The L-NAME-suppressed reactive hyperemia in gingiva was partially recovered by the treatment with L-arginine (60 mg/kg, i.a.), the substrate of NO synthase. Furthermore, NO could be detected using NO-selective electrode during reactive hyperemia in gingival tissue.CONCLUSIONS : These results suggest that NO function as nonadrenergic noncholinergic (NANC) mediator is involved in the vasodilation during reactive hyperemia in gingival tissue, and deals with the maintenance of homeostasis in gingival circulation. Less

反应性充血，由于血流中断引起的氧债和代谢废物积累而发生的局部血管舒张;活动性充血，以及活动期间器官中血流量增加。微循环部分由交感神经控制，部分由内皮细胞局部分泌的血管活性物质控制。后者中最重要的是一氧化氮（NO），其通过引起血管平滑肌松弛来促进流动。本研究采用激光多普勒血流仪和NO电化学技术，探讨了NO在反应性充血期间牙龈组织局部循环调节中的作用。方法：实验在戊巴比妥钠（25 mg/kg，静脉注射）麻醉下的比格犬上进行。用激光多普勒血流仪测量了右下颌尖牙附着牙龈组织的牙龈血流量。诱发反应性充血关于我们艾德通过释放闭合压迫（30、60和300秒周期），激光多普勒流量计探头以小于GBF 2 ml/min/100 g的力施加在牙龈组织上。为了进行药理学考虑牙龈组织中产生的反应性充血，动脉内推注的各种drugsgived.Results：GBF增加取决于闭塞持续时间的30，60，和300秒的牙龈组织压缩。预先腹腔注射N ω-硝基-L-精氨酸甲酯（L-NAME，60 mg/kg）可明显抑制反应性充血，一氧化氮合酶抑制剂，但不被阿托品（毒蕈碱拮抗剂）、普萘洛尔（β-受体阻滞剂）、吡拉明（H1受体阻滞剂）、西咪替丁（H2受体阻滞剂）所抑制。L-NAME抑制的牙龈反应性充血通过L-精氨酸（60 mg/kg，i.a.）治疗部分恢复，NO合成酶的底物。结论：NO作为非肾上腺素能非胆碱能（nonadrenergic noncholinergic，NANC）介质参与了牙龈组织反应性充血时的血管舒张作用，维持牙龈循环的稳态。少