Immunopathological study on cytokine-producing cells in acute oral mucosal graft-versus-host disease

所得结果分为两部分；1)细胞因子在急性口腔黏膜GVHD诱导中的作用；2)免疫活性细胞的动力学，包括细胞因子产生细胞。通过RT-PCR方法，在口腔病变早期病变部位均表达干扰素- γ (IFN)和肿瘤坏死因子- α (TNF) mrna。这些细胞因子的表达与上皮性角质形成细胞（KC）上ICAM-1的免疫组化表达同时发生。这些结果表明，IFN和TNF的局部产生可能是诱导KC上ICAM-1表达的原因。此外，stamer - woodruff结合试验证实了ICAM-1在KC上的表达可调节细胞毒性T细胞（cd8阳性细胞）向上皮的浸润。通过原位杂交，我们推测nk样细胞和巨噬细胞可能分别负责IFN和TNF的产生。根据这些证据，我们得出结论，nk样细胞和巨噬细胞在急性口腔黏膜GVHD的发生中起重要作用。这些细胞产生的细胞因子可能通过LFA-1/ICAM-1结合途径调节细胞毒性T细胞的上皮性。

The results obtained were divided into two parts ; 1) roles of cytokines in the induction of acute oral mucosal GVHD, and 2) dynamics of immunocompetent cells, containg cytokine-producing cells. By the RT-PCR methods, both interferon-gamma (IFN) and tumor necrosis factor-alpha (TNF) mRNAs were expressed in the lesional sites at very early stage of oral lesions. The expression of those cytokines was concomitant with immunohistochemical expression of ICAM-1 on the epithelial keratinocytes (KC). These results suggest that local production of IFN and TNF may be responsible for the induction of ICAM-1 expression on KC.Futhermore, the fact that ICAM-1 expression on KC regulates to an infiltration of cytotoxic T cells (CD8-positive cells) into the epithelium, was proved by Stamper-Woodruff binding assay. Of the cytokine-producing cells, we supeculate that NK-like cells and macrophages may be resposible for the production of IFN and TNF, respectively, by in situ hybridization. From these evidences, 'we conclude that NK-like cells and macrophages play important roles in the initiation of acute oral mucosal GVHD.The cytokines produced by those cells may regulate the epitheliotropism of cytotoxic T cell by LFA-1/ICAM-1 binding pathway.