Possibility of GVT effects on oral malignant nepolasms

GVT 对口腔恶性瘤的影响的可能性

基本信息

批准号：
13671977
负责人：
OHNO Jun
金额：
$ 1.73万
依托单位：
Fukuoka Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

The results obtained were divided into three parts ; 1) effects of MCP-1 producing macrophages on the development of local GVH reactions in the oral mucosa, 2) GVT effects on oral melanoma in mice, and 3) biosynthesis of sialic acid on the terminal portion of glycoconjugates can affect effects of GVT against oral melanoma in mice. First, in local GVH reaction of the oral mucosa, macrophages infiltrate into the upper region of lamina propria after an expression of ICAM-1 in epithelial cells. We suggest that MCP-1, produced by macrophages, may be responsible for the chemotactic factor of CTL into surface epithelia of the oral mucosa. Second, GVT effects may depend on degree of systemic GVH reaction. In advanced stages of systemic GVHD, CTL infiltrated directly into nests of melanoma cells (B16 ; C57BL/6-origin), whereas less infiltration of CTL was noted in the early phase. We suggested that GVT effects were induced by immunological pathway of acute oral mucosal GVHD. However, CTL reaction against tumor cells were not specific, because they also attacked oral mucosa, ispecially epithelial cells. Third, the cell surface glycoconjugates of melanoma cells injected were modified during their development in the oral mucosa. We examined histochemically an attachment of sialic acid on the terminal portion of glycoconjugates in melanoma cells. Binding of sialic acid on the cell surface may responsible for susceptibleness of metastasis into submandibular lymph nodes and for difficulties of GVT effects.

获得的结果分为三个部分； 1）MCP-1产生巨噬细胞对口腔粘膜中局部GVH反应的发展，2）GVT对小鼠口腔黑色素瘤的影响，以及3）唾液酸对糖酮终末部分的生物合成对小鼠口腔瘤的影响可能会影响GVT的影响。首先，在口服粘膜的局部GVH反应中，巨噬细胞在上皮细胞中表达ICAM-1后浸润到层中的上部。我们建议由巨噬细胞产生的MCP-1可能导致CTL趋化因子进入口服粘膜的表面上皮。其次，GVT效应可能取决于全身GVH反应的程度。在系统性GVHD的晚期阶段，CTL直接渗透到黑色素瘤细胞的巢中（B16; C57BL/6-ORIGIN），而在早期，CTL的浸润较少。我们建议通过急性口服粘膜GVHD的免疫学途径诱导GVT效应。但是，CTL对肿瘤细胞的反应不是特异性的，因为它们也攻击了口服粘膜，尤其是上皮细胞。第三，在口服粘膜中发育过程中，对注射的黑色素瘤细胞的细胞表面糖缀合物进行了修饰。我们在组织化学上检查了唾液酸在黑色素瘤细胞中糖缀合物末端的附着。唾液酸对细胞表面的结合可能导致转移对下颌淋巴结的敏感性以及GVT效应的困难。