Function of Lipid-caoted cytokines

二癸基- n -葡萄糖- l-谷氨酸是一种中性脂质，用于包裹某些细胞因子，如b-FGF和BMP。脂质在磷酸盐缓冲液中与b-FGF或BMP反应。紫外分析证实得到的固体分别含有约3%的b-FGF和2%的BMP。脂包被的b-FGF和BMP可溶于氯仿和异丙醚。圆二色性分析证实脂膜b-FGF在有机溶液中的构象是稳定的。将脂包被的b-FGF和BMP植入大鼠胫骨。显微照片显示脂质包被的b-FGF和BMP加速了新骨的形成。合成了l -丙氨酸正癸酯(C10-L-Ala)/对甲苯磺酸盐或l -丙氨酸正癸酯(C12-L-Ala) /对甲苯磺酸盐作为人工两亲性脂类。将DNA与合成的两亲性脂质简单混合制备DNA/C10-L-Ala复合物和DNA/C12-L-Ala复合物，作为脂质包被细胞因子的新载体。这些配合物不溶于水，但可溶于乙醇和氯仿等有机溶剂，在氯仿(CHCl_3)/乙醇（EtOH）溶液中也能形成双螺旋b型结构。以CHCl_3/EtOH溶液为原料，采用浇铸法制备了自立、不溶于水的dna -脂膜。大鼠背部皮下植入3天后，dna -脂质膜基本溶解。没有炎症反应或新组织形成障碍。dna -脂质膜在牙科和医学领域具有广泛的应用前景。

Didecyl-N-glucono-L-glutamate as a neutral lipid was prepared in order to coat some cytokines such as b-FGF and BMP.The lipid was allowed to react with b-FGF or BMP in phosphate buffer solution. UV analysis confirmed that obtained solid contained approximately 3% b-FGF and 2% BMP, respectively. The lipid-coated b-FGF and BMP were soluble in chloroform and isopropyl ether. Circular dichroism analysis confirmed that the conformation of lipid-coated b-FGF in organic solution was stable. The lipid-coated b-FGF and BMP were implanted in rat tibia. Photomicrograph revealed that the lipid-coated b-FGF and BMP accelerated new bone formation. L-alanine n-decyl ester (C10-L-Ala)/p-toluenesulfonic acid salt or L-alanine n-lauryl ester (C12-L-Ala) /p-toluenesulfonic acid salt were synthesized as artificial amphiphilic lipids. The DNA/C10-L-Ala and DNA/C12-L-Ala complexes were prepared from the simple mixing of DNA and synthesized amphiphilic lipids to used as new carrier for the lipid-coated cytokines. These complexes were insoluble in water, but soluble in organic solvents such as ethanol and chloroform, and were confirmed to form a double helical B-form structure even in chloroform (CHCl_3)/ethanol (EtOH) solution. The self-standing, water-insoluble DNA-lipid films were prepared by casting from CHCl_3/EtOH solution. The DNA-lipid films were almost dissolved after 3 days of subcutaneous implantation in the backs of rats. There were no inflammatory reactions or an obstacle to new tissue formation. The DNA-lipid films may be suitable materials in the dental and medical fields.