Function of Lipid-caoted cytokines

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Didecyl-N-glucono-L-glutamate as a neutral lipid was prepared in order to coat some cytokines such as b-FGF and BMP.The lipid was allowed to react with b-FGF or BMP in phosphate buffer solution. UV analysis confirmed that obtained solid contained approximately 3% b-FGF and 2% BMP, respectively. The lipid-coated b-FGF and BMP were soluble in chloroform and isopropyl ether. Circular dichroism analysis confirmed that the conformation of lipid-coated b-FGF in organic solution was stable. The lipid-coated b-FGF and BMP were implanted in rat tibia. Photomicrograph revealed that the lipid-coated b-FGF and BMP accelerated new bone formation. L-alanine n-decyl ester (C10-L-Ala)/p-toluenesulfonic acid salt or L-alanine n-lauryl ester (C12-L-Ala) /p-toluenesulfonic acid salt were synthesized as artificial amphiphilic lipids. The DNA/C10-L-Ala and DNA/C12-L-Ala complexes were prepared from the simple mixing of DNA and synthesized amphiphilic lipids to used as new carrier for the lipid-coated cytokines. These complexes were insoluble in water, but soluble in organic solvents such as ethanol and chloroform, and were confirmed to form a double helical B-form structure even in chloroform (CHCl_3)/ethanol (EtOH) solution. The self-standing, water-insoluble DNA-lipid films were prepared by casting from CHCl_3/EtOH solution. The DNA-lipid films were almost dissolved after 3 days of subcutaneous implantation in the backs of rats. There were no inflammatory reactions or an obstacle to new tissue formation. The DNA-lipid films may be suitable materials in the dental and medical fields.