Application of gel DNA/artificial lipid complex for new scaffolds in tissue regeneration

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The artificial dicationic lipids were synthesized from the reaction of L-alanine, and polymethylene glycohols (PMG) or polyethylene glycohols(PEG). Gel DNA/lipid complexes were prepared by reaction of DNA with cationic lipids. Although they were very low cytotoxic and do not cause an unfavorable tissue response, they were degraded relatively short periods in PBS buffer solution, and in the subcutaneous tissues of rats. It was found that DNA/dication lipid complexes were not suitable for scaffolds, which can induce development of new bone. Therefore, it predicted that the rate at which DNA-containing complexes dissolved in vivo or vitro can be controlled by crosslinking DNA with polycation such as chitosan because they have many functional groups in one molecule. The DNA/chitosan gel complexes were regarded in vivo or in vitro more slowly that DNA/dication lipid complexes and were more suitable for scaffolds than DNA/dication lipid complexes. The DNA/chitosan gel complexes with and without apatite were fabricated in mold by newly PBS buffer solution methods. The DNA/chitosan gel complexes with apatite induced induce development of new bone. Moreover, the DNA/chitosan gel complex treated with cation lipid had antifungal activity, and they will be useful for GTR. The DNA/chitosan gel complex also became transparent film after being pressed. This film will be useful for medical dressing materials and an adhesion barrier.