权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Application of gel DNA/artificial lipid complex for new scaffolds in tissue regeneration

凝胶DNA/人工脂质复合物新型支架在组织再生中的应用

基本信息

批准号：
16390574
负责人：
FUKUSHIMA Tadao
金额：
$ 8.83万
依托单位：
Fukuoka Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

The artificial dicationic lipids were synthesized from the reaction of L-alanine, and polymethylene glycohols (PMG) or polyethylene glycohols(PEG). Gel DNA/lipid complexes were prepared by reaction of DNA with cationic lipids. Although they were very low cytotoxic and do not cause an unfavorable tissue response, they were degraded relatively short periods in PBS buffer solution, and in the subcutaneous tissues of rats. It was found that DNA/dication lipid complexes were not suitable for scaffolds, which can induce development of new bone. Therefore, it predicted that the rate at which DNA-containing complexes dissolved in vivo or vitro can be controlled by crosslinking DNA with polycation such as chitosan because they have many functional groups in one molecule. The DNA/chitosan gel complexes were regarded in vivo or in vitro more slowly that DNA/dication lipid complexes and were more suitable for scaffolds than DNA/dication lipid complexes. The DNA/chitosan gel complexes with and without apatite were fabricated in mold by newly PBS buffer solution methods. The DNA/chitosan gel complexes with apatite induced induce development of new bone. Moreover, the DNA/chitosan gel complex treated with cation lipid had antifungal activity, and they will be useful for GTR. The DNA/chitosan gel complex also became transparent film after being pressed. This film will be useful for medical dressing materials and an adhesion barrier.

以L-丙氨酸为原料，分别与聚亚甲基二醇（PMG）和聚乙二醇（PEG）反应，合成了人工双阳离子脂质。通过DNA与阳离子脂质的反应制备凝胶DNA/脂质复合物。虽然它们的细胞毒性非常低，不会引起不利的组织反应，但它们在PBS缓冲液和大鼠皮下组织中降解的时间相对较短。发现DNA/二价阳离子脂质复合物不适合作为支架材料，其可诱导新骨的发育。因此，它预测，在体内或体外溶解的含DNA的复合物的速度可以通过交联DNA与聚阳离子，如壳聚糖，因为它们在一个分子中有许多官能团控制。DNA/壳聚糖凝胶复合物在体内和体外的反应速度均比DNA/双阳离子脂质复合物慢，更适合作为支架材料。采用PBS缓冲溶液法在模具中制备了含磷灰石和不含磷灰石的DNA/壳聚糖凝胶复合物。DNA/壳聚糖凝胶复合物与磷灰石诱导诱导的新骨发育。经阳离子脂质处理后的DNA/壳聚糖凝胶复合物具有抗真菌活性，可用于GTR的研究。DNA/壳聚糖凝胶复合物经挤压后也成为透明膜。该膜可用于医用敷料材料和防粘连层。