Application of DNA films for Dental Use

以L-丙氨酸和正十二醇为原料，合成了人工阳离子脂类化合物。通过DNA与阳离子脂类的反应制备DNA-脂类复合体，并从氯仿/乙醇溶液中浇铸DNA-脂类复合体制备自立的、不溶于水的DNA-脂膜。DNA-脂膜的细胞毒性很低，不会暂停不利的组织反应。此外，DNA-脂膜对所测试的细菌菌株具有抗菌活性。为此，实验制备了含脂质的MMA液体和含DNA-脂质复合体的PMMA粉末，以赋予MMA-TBB树脂抗菌性能。虽然脂类和DNA-脂类复合体对树脂与酸蚀牙釉质和牙本质的粘结有不利影响，但树脂具有良好的抗菌活性。DNA-脂膜与溴化乙锭形成插层络合物。这表明DNA-脂膜保持双螺旋结构，并且DNA-脂膜有可能在抗生素或细胞因子之间形成插层化合物。DNA膜的拉伸强度为2.0 Mpa。2.2 Mpa，并与用于牙科引导组织再生的商业可获得材料兼容。DNA-脂膜、DNA-脂膜和脂类有望在牙科材料、生物材料和DDS中得到应用。

The artificial cationic lipids were synthesized from the reaction of L-alanine, and n-alkyl alcohol (n-decyl alcohol or n-lauryl alcohol). DNA-lipid complexes were prepared by reaction of DNA with cationic lipids and the self-standing, water-insoluble DNA-lipid films were prepared by casting the DNA-lipid complex from chloroform/ethanol solution. DNA-lipid films were very low cytotoxic and do not pause an unfavorable tissue response. Moreover, DNA-lipid films exhibited antibacterial activity against the bacterial strains tested. Therefore, MMA liquid with lipids and PMMA powder with DNA-lipid complex were experimentally prepared to give antibacterial activity to MMA-TBB resin. Although the lipids and DNA-lipid complex were adversely effect on the bonding of the resins to etched enamel and dentin, the resins had good antibacterial activity. The DNA-lipid films formed intercalation complexes with ethidium bromide. This indicates that DNA-lipid films maintain a double helical structure and DNA-lipid films have a potential to form intercalation compounds between antibiotics or cytokines. The tensile strengths of DNA films were 2.0 MPa to. 2.2 MPa and were compatible with a commercially available material for guided tissue regeneration in dental use. It is expected that the DNA-lipid films, DNA-lipid complex and lipid will be applicable as dental materials, biomaterials and DDS.