Application of DNA films for Dental Use

DNA胶片在牙科中的应用

• 批准号: 13557171
• 负责人: FUKUSHIMA Tadao
• 金额: $ 8.51万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13557171/
• 关键词: DNA; DNA film; Artificial lipids; DNA-lipid complex; Antibacterial activity; Dental material; DDS; DNA複合体; 抗ガン剤; サイトカイン; 人工脂質修飾DNA; 細胞毒性; 生体分解性材料

The artificial cationic lipids were synthesized from the reaction of L-alanine, and n-alkyl alcohol (n-decyl alcohol or n-lauryl alcohol). DNA-lipid complexes were prepared by reaction of DNA with cationic lipids and the self-standing, water-insoluble DNA-lipid films were prepared by casting the DNA-lipid complex from chloroform/ethanol solution. DNA-lipid films were very low cytotoxic and do not pause an unfavorable tissue response. Moreover, DNA-lipid films exhibited antibacterial activity against the bacterial strains tested. Therefore, MMA liquid with lipids and PMMA powder with DNA-lipid complex were experimentally prepared to give antibacterial activity to MMA-TBB resin. Although the lipids and DNA-lipid complex were adversely effect on the bonding of the resins to etched enamel and dentin, the resins had good antibacterial activity. The DNA-lipid films formed intercalation complexes with ethidium bromide. This indicates that DNA-lipid films maintain a double helical structure and DNA-lipid films have a potential to form intercalation compounds between antibiotics or cytokines. The tensile strengths of DNA films were 2.0 MPa to. 2.2 MPa and were compatible with a commercially available material for guided tissue regeneration in dental use. It is expected that the DNA-lipid films, DNA-lipid complex and lipid will be applicable as dental materials, biomaterials and DDS.

以L-丙氨酸和正癸醇、正月桂醇为原料合成了人工阳离子脂质。通过DNA与阳离子脂质反应制备DNA-脂质复合物，并通过从氯仿/乙醇溶液中浇铸DNA-脂质复合物来制备自支撑的、水不溶性的DNA-脂质膜。DNA-脂质膜的细胞毒性非常低，不会暂停不利的组织反应。此外，DNA-脂质膜对所测试的细菌菌株表现出抗菌活性。因此，实验制备了含有脂质的MMA液体和含有DNA-脂质复合物的PMMA粉末，以赋予MMA-TBB树脂抗菌活性。虽然脂质和DNA-脂质复合物对树脂与酸蚀牙釉质和牙本质的结合有不利影响，但树脂具有良好的抗菌活性。DNA-脂质膜与溴化乙锭形成插层复合物。这表明DNA-脂质膜保持双螺旋结构，并且DNA-脂质膜具有在抗生素或细胞因子之间形成嵌入化合物的潜力。DNA膜的拉伸强度为2.0 MPa ~. 2.2 MPa，并与牙科使用的引导组织再生的市售材料兼容。DNA-脂质膜、DNA-脂质复合物和脂质有望作为牙科材料、生物材料和DDS应用。

项目成果

T.Fukushima, T.Hayakaya, Y.Inoue, K.Miyazaki, Y.Okahata: "Intercalation behavior and tensile strength of DNA-lipid films for the dental applications"Biomaterials. 25. 5491-5497 (2004)

T.Fukushima、T.Hayakaya、Y.Inoue、K.Miyazaki、Y.Okahata：“牙科应用中 DNA 脂质膜的嵌入行为和拉伸强度”生物材料。

Y.Inoue, T.Fukushima, T.Hayakawa, H.Takeuchi, H.Kaminishi, K.Miyazaki, Y.Okahata: "Antibacterial Characteristics of Newly Developed Amphiphilic Lipids and DNA-lipid Complexes against Bacteria"Journal of Biomedical Materials Research. 65. 203-208 (2003)

Y.Inoue、T.Fukushima、T.Hayakawa、H.Takeuchi、H.Kaminishi、K.Miyazaki、Y.Okahata：“新开发的两亲性脂质和 DNA-脂质复合物对细菌的抗菌特性”生物医学材料研究杂志。

Y.Inoue, T.Fukushima, T.Hayakawa, H.Takeuchi, H.Kaminishi, K.Miyazaki, Y.Okahata: "Anchibacterials characteristics of newly developed amphiphilic lipids and DNA-lipid complexes against bacteria"J Biomed Mater Res. 65A. 203-208 (2003)

Y.Inoue、T.Fukushima、T.Hayakawa、H.Takeuchi、H.Kaminishi、K.Miyazaki、Y.Okahata：“新开发的两亲性脂质和 DNA-脂质复合物对细菌的抗菌特性”J Biomed Mater Res。

T.Fukushima, T.Hayakaya, Y.Inoue, K.Miyazaki, Y.Okahata: "Intercalation behavior and tensile strength of DNA-lipid films for the dental applications"Biomaterials. (in press). (2004)

T.Fukushima、T.Hayakaya、Y.Inoue、K.Miyazaki、Y.Okahata：“牙科应用中 DNA 脂质膜的嵌入行为和拉伸强度”生物材料。