Application of DNA films for Dental Use

以l -丙氨酸和正烷基醇（正癸醇或正十二醇）为原料，合成了人工阳离子脂质。通过DNA与阳离子脂质反应制备DNA-脂质复合物，并将DNA-脂质复合物从氯仿/乙醇溶液中浇铸制备出独立的不溶于水的DNA-脂质膜。dna -脂质膜是非常低的细胞毒性和不暂停不利的组织反应。此外，dna -脂质膜对所测菌株具有抗菌活性。因此，实验制备了含脂的PMMA液体和含dna -脂复合物的PMMA粉末，以提高MMA- tbb树脂的抗菌活性。虽然脂质和dna -脂复合物对树脂与牙本质和牙釉质的结合有不利影响，但树脂具有良好的抗菌活性。dna -脂质膜与溴化乙啶形成插层配合物。这表明dna -脂质膜保持双螺旋结构，dna -脂质膜有可能在抗生素或细胞因子之间形成插层化合物。DNA膜的拉伸强度为2.0 MPa ~ 2 MPa。2.2 MPa，与市售材料兼容，用于牙科使用的引导组织再生。预计dna -脂膜、dna -脂复合物和脂质将应用于牙科材料、生物材料和DDS。

The artificial cationic lipids were synthesized from the reaction of L-alanine, and n-alkyl alcohol (n-decyl alcohol or n-lauryl alcohol). DNA-lipid complexes were prepared by reaction of DNA with cationic lipids and the self-standing, water-insoluble DNA-lipid films were prepared by casting the DNA-lipid complex from chloroform/ethanol solution. DNA-lipid films were very low cytotoxic and do not pause an unfavorable tissue response. Moreover, DNA-lipid films exhibited antibacterial activity against the bacterial strains tested. Therefore, MMA liquid with lipids and PMMA powder with DNA-lipid complex were experimentally prepared to give antibacterial activity to MMA-TBB resin. Although the lipids and DNA-lipid complex were adversely effect on the bonding of the resins to etched enamel and dentin, the resins had good antibacterial activity. The DNA-lipid films formed intercalation complexes with ethidium bromide. This indicates that DNA-lipid films maintain a double helical structure and DNA-lipid films have a potential to form intercalation compounds between antibiotics or cytokines. The tensile strengths of DNA films were 2.0 MPa to. 2.2 MPa and were compatible with a commercially available material for guided tissue regeneration in dental use. It is expected that the DNA-lipid films, DNA-lipid complex and lipid will be applicable as dental materials, biomaterials and DDS.