Application of DNA films for Dental Use
Application of DNA films for Dental Use
批准号:
13557171
负责人:
FUKUSHIMA Tadao
金额:
$8.51万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2001
资助国家:
日本
项目状态:
已结题
起止时间:
2001 至 2003
中文摘要
以l -丙氨酸和正烷基醇(正癸醇或正十二醇)为原料,合成了人工阳离子脂质。通过DNA与阳离子脂质反应制备DNA-脂质复合物,并将DNA-脂质复合物从氯仿/乙醇溶液中浇铸制备出独立的不溶于水的DNA-脂质膜。dna -脂质膜是非常低的细胞毒性和不暂停不利的组织反应。此外,dna -脂质膜对所测菌株具有抗菌活性。因此,实验制备了含脂的PMMA液体和含dna -脂复合物的PMMA粉末,以提高MMA- tbb树脂的抗菌活性。虽然脂质和dna -脂复合物对树脂与牙本质和牙釉质的结合有不利影响,但树脂具有良好的抗菌活性。dna -脂质膜与溴化乙啶形成插层配合物。这表明dna -脂质膜保持双螺旋结构,dna -脂质膜有可能在抗生素或细胞因子之间形成插层化合物。DNA膜的拉伸强度为2.0 MPa ~ 2 MPa。2.2 MPa,与市售材料兼容,用于牙科使用的引导组织再生。预计dna -脂膜、dna -脂复合物和脂质将应用于牙科材料、生物材料和DDS。
英文摘要
The artificial cationic lipids were synthesized from the reaction of L-alanine, and n-alkyl alcohol (n-decyl alcohol or n-lauryl alcohol). DNA-lipid complexes were prepared by reaction of DNA with cationic lipids and the self-standing, water-insoluble DNA-lipid films were prepared by casting the DNA-lipid complex from chloroform/ethanol solution. DNA-lipid films were very low cytotoxic and do not pause an unfavorable tissue response. Moreover, DNA-lipid films exhibited antibacterial activity against the bacterial strains tested. Therefore, MMA liquid with lipids and PMMA powder with DNA-lipid complex were experimentally prepared to give antibacterial activity to MMA-TBB resin. Although the lipids and DNA-lipid complex were adversely effect on the bonding of the resins to etched enamel and dentin, the resins had good antibacterial activity. The DNA-lipid films formed intercalation complexes with ethidium bromide. This indicates that DNA-lipid films maintain a double helical structure and DNA-lipid films have a potential to form intercalation compounds between antibiotics or cytokines. The tensile strengths of DNA films were 2.0 MPa to. 2.2 MPa and were compatible with a commercially available material for guided tissue regeneration in dental use. It is expected that the DNA-lipid films, DNA-lipid complex and lipid will be applicable as dental materials, biomaterials and DDS.
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T.Fukushima, T.Hayakaya, Y.Inoue, K.Miyazaki, Y.Okahata: "Intercalation behavior and tensile strength of DNA-lipid films for the dental applications"Biomaterials. 25. 5491-5497 (2004)
T.Fukushima、T.Hayakaya、Y.Inoue、K.Miyazaki、Y.Okahata:“牙科应用中 DNA 脂质膜的嵌入行为和拉伸强度”生物材料。
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通讯作者:
Y.Inoue, T.Fukushima, T.Hayakawa, H.Takeuchi, H.Kaminishi, K.Miyazaki, Y.Okahata: "Antibacterial Characteristics of Newly Developed Amphiphilic Lipids and DNA-lipid Complexes against Bacteria"Journal of Biomedical Materials Research. 65. 203-208 (2003)
Y.Inoue、T.Fukushima、T.Hayakawa、H.Takeuchi、H.Kaminishi、K.Miyazaki、Y.Okahata:“新开发的两亲性脂质和 DNA-脂质复合物对细菌的抗菌特性”生物医学材料研究杂志。
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通讯作者:
Y.Inoue, T.Fukushima, T.Hayakawa, H.Takeuchi, H.Kaminishi, K.Miyazaki, Y.Okahata: "Anchibacterials characteristics of newly developed amphiphilic lipids and DNA-lipid complexes against bacteria"J Biomed Mater Res. 65A. 203-208 (2003)
Y.Inoue、T.Fukushima、T.Hayakawa、H.Takeuchi、H.Kaminishi、K.Miyazaki、Y.Okahata:“新开发的两亲性脂质和 DNA-脂质复合物对细菌的抗菌特性”J Biomed Mater Res。
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通讯作者:
T.Fukushima, T.Hayakaya, Y.Inoue, K.Miyazaki, Y.Okahata: "Intercalation behavior and tensile strength of DNA-lipid films for the dental applications"Biomaterials. (in press). (2004)
T.Fukushima、T.Hayakaya、Y.Inoue、K.Miyazaki、Y.Okahata:“牙科应用中 DNA 脂质膜的嵌入行为和拉伸强度”生物材料。
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作者:
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通讯作者:
Effect of the cells transformed by elastins on facilitation of DNA/protamine complex-induced bone regeneration
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批准号:25670838
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项目类别:Grant-in-Aid for Challenging Exploratory Research
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资助金额:$2.33万
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财政年份:2013
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负责人:FUKUSHIMA Tadao
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依托单位:
Development of injectable scaffolds including DNA/polycation complexes
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批准号:19390505
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$11.9万
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财政年份:2007
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负责人:FUKUSHIMA Tadao
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依托单位:
Application of gel DNA/artificial lipid complex for new scaffolds in tissue regeneration
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批准号:16390574
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$8.83万
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财政年份:2004
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负责人:FUKUSHIMA Tadao
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依托单位:
Development of functionally graded DNA films containing cytokines eliciting bone formation
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批准号:13672070
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.62万
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财政年份:2001
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负责人:FUKUSHIMA Tadao
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依托单位:
Function of Lipid-caoted cytokines
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批准号:11671968
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.11万
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财政年份:1999
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负责人:FUKUSHIMA Tadao
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依托单位:
Simulation of Permeability for Adhesive Monomer into Etched Dentin
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批准号:01571081
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.22万
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财政年份:1989
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负责人:FUKUSHIMA Tadao
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依托单位:
海外基金