Development of functionally graded DNA films containing cytokines eliciting bone formation

含有诱导骨形成的细胞因子的功能分级 DNA 膜的开发

• 批准号: 13672070
• 负责人: FUKUSHIMA Tadao
• 金额: $ 2.62万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672070/
• 关键词: DNA; Lipid; Cytokine; DNA film; DNA-lipid complex; Antibacterial activity; Intercalation; Tensile strength

The DNA-lipid complexes with and without cytokines BMP and b-FGF were prepared synthetic lipids from the reaction of n-alkyl alcohol (C=4〜C=20) and amino acids (glycine, L-alanine and L-glutamic acids) and the DNA films were obtained by casting the DNA -lipid complexes from chloroform/ethanol solution. The tensile strengths and intercalation behavior of DNA films were investigated. Antibacterials characteristics of DNA-lipid complexes against bacteria (P. gingivalis, S. mutans, S. aureus and P. aeruginosa) were also investigated. Additionally, effect of the DNA -lipid complexes with cytokines BMP and b-FGF on bone formation was evaluated. The DNA-lipid films had tensile strengths of approximately 2MPa, and formed intercalation complexes with ethidium bromide. The formation of the intercalation complex of DNA-lipid film with ethidium bromide was influenced by the length of the alkyl chains of the synthesized lipids. The complexes prepared from DNA with glycine decyl ester or glycine dodecyl ester, and with L-alanine decyl ester or glycine dodecyl ester showed antibacterial activity. However, the DNA-lipid complexes with cytokines BMP and b-FGF did not elicit bone formation.

用正烷基醇(C=4~C=20)与氨基酸(甘氨酸、L-丙氨酸和L-谷氨酸)反应制备含和不含细胞因子骨形态发生蛋白和b-成纤维细胞生长因子的脱氧核糖核酸-脂质复合体，并用氯仿/乙醇溶液浇铸得到脱氧核糖核酸膜。研究了DNA薄膜的拉伸强度和插层行为。研究了DNA-脂质复合体对牙龈假单胞菌、变形链球菌、金黄色葡萄球菌和铜绿假单胞菌的抑菌特性。此外，还评价了细胞因子BMP和b-FGFDNA-脂质复合体对骨形成的影响。DNA-脂膜的拉伸强度约为2 Mpa，并与溴化乙锭形成了插层络合物。合成脂质的烷基链长对DNA-脂膜与溴化乙锭形成插层复合体有一定的影响。与甘氨酸正十二酯、甘氨酸十二酯、L-丙氨酸正十二酯、甘氨酸十二酯形成的配合物均具有一定的抗菌活性。然而，含有细胞因子BMP和b-FGF的DNA-脂质复合体不能诱导骨形成。