Effects of exercise and cold exposure on branched-chain amino acids catabolism

运动和寒冷暴露对支链氨基酸分解代谢的影响

• 批准号: 11680048
• 负责人: FUJII Hisao
• 金额: $ 0.45万
• 依托单位: Sendai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680048/
• 关键词: Cold exposure; Exercise training; Branched-chain amino acids catabolism; Rat skeletal muscle; Branched-chain α-keto acid dehydrogenase; 分岐鎖α-ケト酸脱水素酵素複合体; 環境温度ストレス; 寒冷環境; 運動; ラット

The stress of cold exposure increases utilization of protein for energy. Exercise training also promotes protein degradation in skeletal muscle and increases its amino acids catabolism. Therefore, protein consumption in cold-condition sports is greater compared to that in comfortable-condition sports, and the important muscles for performance may be damaged. We examined the effects of exercise training and the stress of cold exposure on a branched-chain α-keto acid dehydrogenase (BCKD) activity in rat skeletal muscle, which is the rate-limiting enzyme of branched-chain amino acids (BCAAs) catabolism. Total activity and activity state (actual activity / total activity) of BCKD in rat skeletal muscle were significantly elevated by exercise training in cold exposure for 3 weeks. These results suggest that exercise training in cold exposure for 3 weeks enhances not only BCAAs catabolism under the resting condition but also the capacity for catabolism during exercise in rat skeletal muscle. In addition, cold exposure for 3 weeks significantly inhibited weight gain. But exercise training in cold exposure 3 weeks led to improve the stagnation of weight gain.

冷暴露的应激增加了蛋白质的能量利用。运动训练还促进骨骼肌中蛋白质的降解，并增加其氨基酸催化剂。因此，在寒冷条件下运动的蛋白质消耗量比在舒适条件下运动的蛋白质消耗量更大，并且可能会损坏用于性能的重要肌肉。研究了运动训练和冷暴露应激对大鼠骨骼肌支链氨基酸（BCAA）催化剂的限速酶--支链α-酮酸脱氢酶（BCKD）活性的影响。冷暴露3周的运动训练可显著提高大鼠骨骼肌BCKD的总活性和活性状态（实际活性/总活性）。这些结果表明，3周的冷暴露运动训练不仅增强了大鼠骨骼肌在静息状态下的BCAAs催化能力，而且增强了运动过程中的BCAAs催化能力。此外，冷暴露3周显着抑制体重增加。但冷暴露3周运动训练导致体重增加停滞改善。