Single cell glycomics: mapping the surface glycan signature of cells

单细胞糖组学：绘制细胞表面聚糖特征

• 批准号: DP200102004
• 负责人: Prof Yuling Wang
• 金额: $ 28.86万
• 依托单位: Macquarie University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2020
• 资助国家: 澳大利亚
• 起止时间: 2020-05-18 至 2023-05-17
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP200102004
• 关键词: Single; cell; glycomics; mapping; surface

This project aims to develop a platform technology for multiplexed glycan mapping of the surface of a single cell to address challenges of functional glycomics by utilising a conceptually new approach. By combining newly designed plasmonic nanoparticles with surface-enhanced Raman scattering tags and multiple specific carbohydrate-recognising lectins, this project expects to produce a generic technology that is capable of non-destructive barcoding of the surface glycan signature of single cells in their native state and in response to metabolic perturbations. Expected project outcomes include advancing knowledge in nanobiotechnology, glycobiology and cell biology by being able to easily monitor changes to the surface of single cells.

该项目旨在开发一个平台技术，用于单细胞表面的多路聚糖映射，通过利用概念上的新方法来解决功能性糖组学的挑战。通过将新设计的等离子体纳米粒子与表面增强的拉曼散射标签和多种特定的碳水化合物识别凝集素相结合，该项目预计将产生一种通用技术，能够对单细胞在天然状态和代谢扰动下的表面聚糖特征进行非破坏性条形码。预期的项目成果包括通过能够轻松地监测单细胞表面的变化来推进纳米生物技术、糖生物学和细胞生物学方面的知识。