Development of Integrated Micro-Chip Device for DNA Analysis

DNA芯片系统的开发，可以在小型医疗机构中，无需专业技能，即可对个体遗传信息进行即时、高精度的分析，使医生能够通过准确的发育前诊断，及早进行治疗，防止病情进一步发展。预计这将大大有助于预防疾病和维持人民的健康状况。我们的研究目标是开发高精度的微积累DNA芯片，用于基因分析，准确诊断疾病前期发展。DNA芯片由扩增待分析DNA的PCR和利用杂交的分析部分组成。今年，我们开发了微pcr设备和新的DNA分析技术。通过在3cm×3cm的玻璃板上加工微波路线，我们创造了温度循环约为30次的微型PCR设备。我们还创造了微型加热器，用于…通过在加工波路的玻璃板背面形成ITO膜来控制温度。通过将芘酸溶液送入微波通道，并对微通道内的温度分布进行分析，通过测量微观荧光，发现ITO薄膜可以有效地作为PCR芯片的微加热器。另外，以大肠杆菌的DNA为模板进行PCR，证实了国产PCR尖端与市场上的PCR尖端一样可以扩增。此外，我们还开发了利用金胶体进行DNA杂交分析的新技术。将边缘装饰有金胶体的证明DNA固定在金层上，并利用表面等离子体共振进行了光谱测量。因此，该实验使我们能够观察到伴随杂交的大SPR信号。通过研究方法进一步考察检测限，确认了pM水平检测的有效性。此外，该方法对snp检测的有效性也得到了证实；因此，该实验方法被证明适用于DNA检测。我们今年的研究成果是建立了微PCR创建方法和成功开发了新的DNA杂交检测技术。少

The development of DNA chip system, which can carry out immediate analysis on an individual genetic information with high accuracy without specialized skill in minor medical institutions, could enable a doctor to perform the early treatment and prevent it from further developing with its accurate pre-development diagnosis. This is expected to contribute greatly to the disease prevention and maintenance of health conditions of a people.Our research aims at the development of high accuracy micro-accumulation DNA chip, which can be applicable to a gene analysis for an accurate pre-disease development diagnosis. DNA chip consists of PCR, which amplifies DNA to be analyzed, and analyzing portion, which uses hybridization. This year, we have developed micro-PCR equipment and new DNA analysis teqnique.We nave created micro PCR equipment whose temperature cycles are about as many as 30 times by processing the microwave route on the glass board of 3cm×3cm Micro-heater has also been created for … More temperature control by forming ITO film on the reverse side of the glass board on which a wave route has been processed. Analysis on the temperature profile in the micro channel by sending pyrenes acid solution into the microwave route and by measuring microscopic fluorescence has revealed that ITO film is effectively functioned as the micro heater of PCR chip. Also, carrying out PCR of DNA whose origin is a colon bacillus as template has made us confirm that the home-developed PCR tip can be amplified as much as the one on the market.Also, we have developed new DNA hybridization analysis technique using gold colloid. The prove DNA decorated with the gold colloid at the edge has been fixed on the gold layer and spectrum measurement using surface plasmon resonance has been carried out. This experiment consequently has enabled us to observe large SPR signal accompanied by hybridization. Further examination of detection limit by our research method makes us confirm the availability of pM level-detection. Furthermore, the effectiveness for SNPs detection has also been confirmed ; therefore, this experimental method is proved to be applicable to DNA detection.Our research achievements of this year are the establishment of micro PCR creation tequnique and the successful development of new DNA hybridization detection technique. Less