Development of Integrated Micro-Chip Device for DNA Analysis

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The development of DNA chip system, which can carry out immediate analysis on an individual genetic information with high accuracy without specialized skill in minor medical institutions, could enable a doctor to perform the early treatment and prevent it from further developing with its accurate pre-development diagnosis. This is expected to contribute greatly to the disease prevention and maintenance of health conditions of a people.Our research aims at the development of high accuracy micro-accumulation DNA chip, which can be applicable to a gene analysis for an accurate pre-disease development diagnosis. DNA chip consists of PCR, which amplifies DNA to be analyzed, and analyzing portion, which uses hybridization. This year, we have developed micro-PCR equipment and new DNA analysis teqnique.We nave created micro PCR equipment whose temperature cycles are about as many as 30 times by processing the microwave route on the glass board of 3cm×3cm Micro-heater has also been created for … More temperature control by forming ITO film on the reverse side of the glass board on which a wave route has been processed. Analysis on the temperature profile in the micro channel by sending pyrenes acid solution into the microwave route and by measuring microscopic fluorescence has revealed that ITO film is effectively functioned as the micro heater of PCR chip. Also, carrying out PCR of DNA whose origin is a colon bacillus as template has made us confirm that the home-developed PCR tip can be amplified as much as the one on the market.Also, we have developed new DNA hybridization analysis technique using gold colloid. The prove DNA decorated with the gold colloid at the edge has been fixed on the gold layer and spectrum measurement using surface plasmon resonance has been carried out. This experiment consequently has enabled us to observe large SPR signal accompanied by hybridization. Further examination of detection limit by our research method makes us confirm the availability of pM level-detection. Furthermore, the effectiveness for SNPs detection has also been confirmed ; therefore, this experimental method is proved to be applicable to DNA detection.Our research achievements of this year are the establishment of micro PCR creation tequnique and the successful development of new DNA hybridization detection technique. Less