Development of Nonoactuators by Using of Light Radiation Pressure

利用光辐射压力开发非致动器

• 批准号: 13305018
• 负责人: MISAWA Hiroaki
• 金额: $ 36.03万
• 依托单位: HOKKAIDO UNIVERSITY (2003)The University of Tokushima (2001-2002)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13305018/
• 关键词: Nanomachine; Nanoactuator; Photomanipulation; DNA; Gold Colloid; Surface Plasmon Resonance

In this research, while clarifying the condensed matter physics of a DNA chain, nanoactuators using the DNA chain were developed1.To make a DNA chain expand and contract by radiation forces as driving forces, it is necessary to bind a gold nano-colloid particle at the end of the DNA chain fixed on gold thin film (substrate) first of all. For binding between either the gold colloid or the gold film and the DNA chain, antigen-antibody reaction between biotin at the end of the DNA and streptavidine on the Au colloid or binding reaction between gold (substrate) and thiol group at the another end of the DNA was used, respectively.2.The DNA flexible operation system using the optical manipulation equipment which consists of Nd:YAG laser and an optical microscope was developed. When manipulation operation of λ DNA was preformed in aqueous solution using this system, it found out that capture and movement operation of the DNA molecule were possible using light.3.To detect the surface plasmon resonance (SPR) signals in controlling the distance between gold thin film substrate-gold colloid by light, the SPR measurement system wish He-Ne laser as a light source was developed. In the SPR measurement, remarkable difference in the SPR signal between the single-and double-strand DNA appeared. It is suggested that the difference arises in the physical properties (length and elasticity) of the DNA molecule according to a chain condition.4.By repeating of the method described in the section I above, controllable multiple the DNA-gold colloid layers can be fabricated. Remarkable positive SPR angular shift was observed with increase of the layer numbers, and enhancement of the SPR signal accompanying multilayering was shown. Therefore, the enhancement of the SPR signal suggests that the multiplication of the DNA-gold colloid layer. This research has shown that DNA molecules were promising as nanoactuator materials.

本研究在阐明DNA链的凝聚态物理的同时，开发了使用DNA链的纳米致动器1。为了使DNA链以辐射力为驱动力进行伸缩，首先需要在固定在金薄膜（基板）上的DNA链的末端结合金纳米胶体粒子。对于金胶体或金膜与DNA链之间的结合，分别采用DNA末端的生物素与Au胶体上的链霉亲和素之间的抗原抗体反应或金（底物）与DNA另一端的巯基之间的结合反应。2.研制了由Nd：YAG激光器和光学显微镜组成的光学操纵装置的DNA柔性操作系统。在水溶液中对λ DNA进行操纵操作时，发现可以利用光对DNA分子进行捕获和移动操作。3.为了检测光控制金薄膜基底-金胶体之间距离的表面等离子体共振（SPR）信号，研制了以He-Ne激光器为光源的SPR测量系统。在SPR测量中，单链和双链DNA之间的SPR信号出现显著差异。这表明DNA分子的物理性质（长度和弹性）根据链条件而不同。4.通过重复上述第I节中描述的方法，可以制备可控的多层DNA-金胶体层。随着膜层数的增加，SPR角位移显著增加，且多层膜的SPR信号增强。因此，SPR信号的增强表明DNA-金胶体层的倍增。这一研究表明，DNA分子是很有前途的纳米致动器材料。

项目成果

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