权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

EFFECT OF ADMINISTRATION OF GNRH ANTAGONIST ON THE DEVELOPMENT OF CAPRINE CORPUS LUTEUM AND CLARIFICATION OF ITS MECHANISM

GNRH拮抗剂对山羊黄体发育的影响及其机制的阐明

基本信息

批准号：
11836009
负责人：
KAWATE Noritoshi
金额：
$ 2.3万
依托单位：
OSAKA PREFECTURE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11836009/
关键词：
goat corpus luteum development of corpus luteum GnRH antagonist LH LH receptor VEGF PGI_2 PGI2 ヤギプロジェステロン

项目摘要

In the first year of the research project, to clarify the roles of luteinizing hormone (LH) in the development and maintenance of caprine corpus luteum, the effects of GnRH antagonist administration on concentrations of LH and progesterone in blood were examined. As the results, in the goat, it has been suggested that endogenous LH is required for both of the development and maintenance of corpus luteum, and that the corpus luteum is entirely dependent on LH for its maintenance but less dependent on LH for the early development of the corpus luteum.In the second year of the research project, to elucidate the mechanism of corpus luteum development, changes of gene expressions of LH receptor, vascular endothelial growth factor (VEGF), CD34 (a specific marker for endothelial cells), prostacyclin (PGI_2) synthase were examined. Furthermore, the effect of GnRH antagonist administration on those genes' expressions in order to examine the roles of endogenous LH for those genes' expressions in the caprine corpus luteum. As the results, it has been suggested that endogenous LH up-regulates the amounts of LH receptor mRNA and protein in the development of the caprine corpus lutuem. It is possible that VEGF_<165> could contribute enhancement of vascular permeability in the period of maintenace of the caprine corpus luteum, since its gene expression is enhanced in the period of maintenance rather than the period of development of the corpus luteum. It is likely that PGI_2 can stimulate the very eary phase of corpus luteum development in the goat, because gene expression of PGI_2 synthase and metabolite of PGI_2 are increased just after the ovulation. Moreover, it has been indicated that the gene expressions of VEGF, CD34 and PGI_2 synthase are regulated independently of endogenous LH.

在研究项目的第一年，为了阐明促黄体生成素（LH）在山羊黄体发育和维持中的作用，研究了GnRH拮抗剂给药对血液中LH和孕酮浓度的影响。结果表明，山羊黄体的发育和维持都需要内源性LH，黄体的维持完全依赖LH，而黄体的早期发育对LH的依赖性较低。为了阐明山羊黄体发育的机制，本研究在第二年对LH受体基因表达的变化进行了研究，检测血管内皮生长因子（VEGF）、内皮细胞特异性标志物CD34、前列环素（PGI_2）合成酶。进一步研究GnRH拮抗剂对这些基因表达的影响，以探讨内源性LH在山羊黄体中对这些基因表达的作用。结果表明，内源性LH在山羊黄体发育过程中上调LH受体mRNA和蛋白的表达。在<165>山羊黄体维持期VEGF基因表达增强，而在黄体发育期VEGF基因表达增强，提示VEGF可能参与了黄体维持期血管通透性的增强。PGI_2可能在山羊黄体发育的早期就具有刺激作用，因为PGI_2合成酶及其代谢产物的基因表达在山羊排卵后立即增加。此外，VEGF、CD 34和PGI_2合酶的基因表达与内源性LH无关。