ELUCIDATION OF REGULATION OF VASCULAR ENDOTHELIAL GROWTH FACTOR EXPRESSION IN THE LUTEAL DEVELOPMENT OF GOATS.

阐明山羊黄体发育中血管内皮生长因子表达的调节。

• 批准号: 13660324
• 负责人: KAWATE Noritoshi
• 金额: $ 1.98万
• 依托单位: OSAKA PREFECTURE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660324/
• 关键词: goat; corpus luteum; development of corpus luteum; VEGF receptors; PGI_2 receptors; prostaglandin; 山羊; PG合成酵素; PGI_2レセプター; 遺伝子発現; VEGF; PGI2

In the first year of the research project (2001), changes of gene expression of the VFGF receptor, or Flk-1 and Flt-1 were examined during the development of caprine corpora lutea to elucidate any regulation mechanism of VEGF signaling system. The results showed that the gene expression of Flk-1, which seems to play a role for transduction of VEGF signaling, increased in the early stage of the luteal development. However, the gene expression of Flt-1, which seems to act as a decoy receptor for VEGF, did not change during the luteal development and increased in the period of luteal maintenance. These results suggest that VEGF may stimulate angiogenesis partly through a VEGF receptor, Flk-1, during The luteal development in the goat and that the signal transduction of VEGF may be regulated at the level of the receptor.In the second year of the research project (2002), to elucidate roles of prostaglandins (PG) in the development of caprine corpora lutea and to look for a method to regress … More the early corpora lutea, changes of gene expressions ofPG synthases and PGI_2 receptors, and effects of PG synthase inhibitors on luteal development were examined. As the results, it was shown that both mRNAs of the PGI_2 receptors, or IP-R and PPARδ increased in the early stage of the luteal development. Furthermore, the result also showed that the luteal development was partially inhibited by the direct administration of phenylbutazone, a PG inhibitor, into the corpus luteum just after ovulation, but the inhibition was not complete. These results suggest that PG, especially PGI_2, may play roles in the luteal development in the goat, but further studies are required to find a method for regression of the early corpus luteum.In conclusion, the present studies indicate a possibility that the increase of gene expression of a VEGF receptor, Flk-1, may participate in regulation of signal transduction of VEGF in the period of luteal development in goats. Besides, these studies suggest that PGs, especially PGI_2, may play a role in the luteal development. Less

在研究项目的第一年（2001年），研究了山羊黄体发育过程中VFGF受体或Flk-1和Flt-1基因表达的变化，以阐明VEGF信号系统的任何调节机制。结果表明，在黄体发育的早期，Flk-1基因的表达增加，这似乎是VEGF信号转导的作用。然而，Flt-1的基因表达，这似乎是作为一个诱饵受体的VEGF，并没有改变在黄体发育和黄体维持期增加。这些结果表明，VEGF在山羊黄体发育过程中可能部分通过其受体Flk-1促进血管生成，VEGF的信号转导可能在受体水平上受到调节。 ...更多信息 结果表明，PG合成酶抑制剂对早期黄体发育有明显的抑制作用，PG合成酶和PGI_2受体基因表达的变化对早期黄体发育有明显的影响。结果表明，PGI_2受体（IP-R）和过氧化物酶体增殖物激活体受体（PPARδ）的mRNA在黄体发育的早期即表达增加。此外，结果还表明，在排卵后立即将PG抑制剂保泰松（phenylbutazone）直接注入黄体，可部分抑制黄体发育，但抑制不完全。这些结果提示，PG，尤其是PGI_2可能在山羊黄体发育过程中起一定作用，但早期黄体的消退尚需进一步研究，提示VEGF受体Flk-1基因表达的增加可能参与了山羊黄体发育过程中VEGF信号转导的调节。此外，这些研究还提示PGs，尤其是PGI_2，可能在黄体发育中起一定作用。少

项目成果

Kawate N, Tsuji M, Tamada H, Inaba T, Sawada T: "Changes of messenger RNAs encoding vascular endothelial growth factor and its receptors during the development and maintenance of caprine corpora lutea"Molecular Reproduction and Development. 64. 166-171 (2

Kawate N、Tsuji M、Tamada H、Inaba T、Sawada T：“在山羊黄体发育和维持过程中编码血管内皮生长因子及其受体的信使 RNA 的变化”分子繁殖和发育。

Kawate N, Tsuji M, Tamada H, Inaba T, Sawada T.: "Enhancement of prostacyclin synthesis at the beginning of formation of caprine corpora lutea."Mol Reprod Dev. (in press).

Kawate N、Tsuji M、Tamada H、Inaba T、Sawada T.：“在山羊黄体形成开始时增强前列环素合成。”Mol Reprod Dev。

Kawate N, Tsuji M, Tamada H, Inaba T, Sawada T: "Inhibition of luteinizing hormone receptor expression during the development of caprine corpora lutes by administration of gonadotropin-releasing hormone antagonist"Molecular Reproduction and Development. 6

Kawate N、Tsuji M、Tamada H、Inaba T、Sawada T：“通过施用促性腺激素释放激素拮抗剂抑制山羊黄体发育过程中的黄体生成激素受体表达”分子生殖和发育。

Kawate, N., Tsuji, M., Tamada, H., Inaba, T. & Sawada, T.: "Changes of messenger RNAs encoding vascular endothelial growth factor and its receptors during the development and maintenance of caprine corpora lutea"Mol. Reprod. Dev.. 64. 166-171 (2003)

川手，N.，辻，M.，玉田，H.，稻叶，T.

Kawate N, Yamazaki M, Tamada H, Inaba T, Sawada T: "Effect of low dose of hCG on induction of fertile estrus in Shiba goats pretreated intravaginally with progesterone during the early postpartum nursing period"Journal of Reproduction and Development. 48.

Kawate N、Yamazaki M、Tamada H、Inaba T、Sawada T：“低剂量 hCG 对产后早期哺乳期经阴道内用黄体酮预处理的柴山羊可育发情诱导的影响”生殖与发育杂志。