MOLECULAR ANALYSIS OF CELL BARRIER ASSOCIATED PROTEIN BARMOTIN (7H6 ANTIGEN)

细胞屏障相关蛋白巴莫丁（7H6 抗原）的分子分析

• 批准号: 11680702
• 负责人: KOKAI Yasuo
• 金额: $ 2.37万
• 依托单位: SAPPORO MEDICAL UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680702/
• 关键词: Tight junction; barmotin; occludin; gap junction; connexin 32; barrier; H.pylori; 細胞バリア; タイト結合; 7H6; バーモチン; アクチン; バリア; フェンス; 疾患

Tight junctions localize uppermost of apposing epithelium and a class of endothelium. Tight junctions serve as a barrier for paracellular pathway and regulate traffics of ion, small molecules and cells. In the present projects, we studied molecular aspects of tight junction related proteins and its role in pathological conditions, such as bacterial gastritis by H.pylori. To perform this, we developed a model to study pathological role of tight junctions in human disease. Study using H.pylori adapted for murine stomach revealed tight junctions play an important role for these bacteria to colonize in the murine stomach. This observation suggests that up-regulation of paracellular barrier of gastric mucosa may prevent H.pylori colonization which leads to neutrophilic gastritis and strongly links to gastric carcinoma and lymphoma. We also elucidated that molecular regulation of tight junction related molecules by gap junction molecule, designated Cx32. This observation has opened up an opportunity to dissect how tight junctions develop and regulate as a member of junctional complex at the cell-cell contacts.

紧密连接位于并置上皮和一类内皮的最上部。紧密连接作为细胞旁通路的屏障，调节离子、小分子和细胞的运输。在本项目中，我们研究了紧密连接相关蛋白的分子方面及其在病理条件下的作用，如幽门螺杆菌引起的细菌性胃炎。为此，我们开发了一种模型来研究紧密连接在人类疾病中的病理作用。使用适应于小鼠胃的幽门螺杆菌的研究揭示了紧密连接对于这些细菌在小鼠胃中的定殖起重要作用。提示胃粘膜细胞旁屏障的上调可能阻止幽门螺杆菌的定植，幽门螺杆菌定植导致嗜酸性胃炎，并与胃癌和淋巴瘤密切相关。我们还阐明了间隙连接分子（命名为Cx32）对紧密连接相关分子的分子调节。这一观察结果为深入研究紧密连接作为细胞间连接复合体的一员是如何发展和调节的提供了机会。

项目成果

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Igarashi, Y.等人：“胶质细胞系衍生的神经营养因子诱导形成血脑屏障的内皮细胞的屏障功能”Biochem。

Kojima, T. et al.: "Induction of tight junctions in human connexin32(hCx32)-transfected mouse hepatocytes: connexin32 interacts with occludin."Biochem. Biophys. Res. Com.. 226. 222-229 (1999)

Kojima, T. 等人：“在人 connexin32 (hCx32) 转染的小鼠肝细胞中诱导紧密连接：connexin32 与 occludin 相互作用。”Biochem。

Kojima, T. et al.: "Cx32 but not Cx26 is associated with tight junctions in primary cultures of rat hepatocytes."Exp.Cell Res.. 263. 193-201 (2001)

Kojima, T. 等人：“Cx32 但不是 Cx26 与大鼠肝细胞原代培养物中的紧密连接相关。”Exp.Cell Res.. 263. 193-201 (2001)

Suzuki,K. et al.: "SS1 Helicobactor pylori disrupts the paracellular barrier of the gastric mucosa and leads to neutrophilic gastritis in mice."Virchows.Archiv.. (印刷中).

Suzuki, K. 等人：“SS1 幽门螺杆菌会破坏胃粘膜的细胞旁屏障，导致小鼠中性粒细胞性胃炎。”Virchows.Archiv..（出版中）。