MOLECULAR ANALYSIS OF CELL BARRIER ASSOCIATED PROTEIN BARMOTIN (7H6 ANTIGEN)

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Tight junctions localize uppermost of apposing epithelium and a class of endothelium. Tight junctions serve as a barrier for paracellular pathway and regulate traffics of ion, small molecules and cells. In the present projects, we studied molecular aspects of tight junction related proteins and its role in pathological conditions, such as bacterial gastritis by H.pylori. To perform this, we developed a model to study pathological role of tight junctions in human disease. Study using H.pylori adapted for murine stomach revealed tight junctions play an important role for these bacteria to colonize in the murine stomach. This observation suggests that up-regulation of paracellular barrier of gastric mucosa may prevent H.pylori colonization which leads to neutrophilic gastritis and strongly links to gastric carcinoma and lymphoma. We also elucidated that molecular regulation of tight junction related molecules by gap junction molecule, designated Cx32. This observation has opened up an opportunity to dissect how tight junctions develop and regulate as a member of junctional complex at the cell-cell contacts.