MOLECULAR MAPPING OF TIGHT JUNCTION PROTEINS WITH RECOMBINANT 7H6 POLYPEPTIDE

用重组 7H6 多肽对紧密连接蛋白进行分子图谱分析

• 批准号: 08680771
• 负责人: KOKAI Yasuo
• 金额: $ 0.58万
• 依托单位: SAPPORO MEDICAL UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08680771/
• 关键词: tight junction; barmotin; oclcudin; zo-1; cancer; barrier; 分子病理学; ZO-1; Barmotin; 生体バリア; 分子モーター; SMC; 生体防御

Tight junctions localize uppermost of apposing epithelium and a class of endothelium. Tight junctions serve as a barrier for paracellular pathway and regulate traffics of ion, small molecules and cells. In the present projects, we studied molecular structure of tight junction proteins and those expression in normal and disordered tissues both in vivo and in vitro. To perform these studies, we cloned rat occludin and produced polyclonal antibodies against barmotin and occludin. As barmotin has been suggested to belong SMC mechanochemical motor protein, we identified four more SMC proteins in human and rats. Study using human colon cancer revealed that abberant expression of tight junction proteins were observed. The pattern of dysregulation of tight junction proteins were also observed in mouse intestinal cell lines upon treatment of bacterial lipopolysaccharide. Taken together, these observations suggested that tight junction proteins play an important role in both normal and disordered tissues. The current study has elicited the role of tight junction proteins in various conditions.

紧密连接位于相邻上皮和一类内皮细胞的上部。紧密连接作为细胞旁通路的屏障，调节离子、小分子和细胞的交通。在本项目中，我们研究了紧密连接蛋白的分子结构以及在体内和体外正常组织和紊乱组织中的表达。为了进行这些研究，我们克隆了大鼠occludin，并制备了抗barmotin和occludin的多克隆抗体。由于barmotin被认为属于SMC机械化学运动蛋白，我们在人和大鼠中又鉴定了4种SMC蛋白。对人结肠癌的研究发现紧密连接蛋白的异常表达。细菌脂多糖在小鼠肠细胞系中也观察到紧密连接蛋白的失调模式。综上所述，这些观察结果表明紧密连接蛋白在正常和紊乱组织中都起着重要作用。目前的研究已经揭示了紧密连接蛋白在各种条件下的作用。

项目成果

Kimura H., Sawada N., Tobioka H., Isomura H., Kokai Y., Hirata K., Mori M.: "Bacterial lipopolysaccharide reduced interstinal barrier function and altered localization of 7H6 antigen in IEC-6 intestinal crypt cells" Journal of Cellular Physiology. 171. 28

Kimura H.、Sawada N.、Tobioka H.、Isomura H.、Kokai Y.、Hirata K.、Mori M.：“细菌脂多糖降低间质屏障功能并改变 IEC-6 肠隐窝细胞中 7H6 抗原的定位”期刊

Kimura,H.et al.: "Bacterial lipopolysaccharide reduced intestinal barrier function and altered localization of 7H6 antigen in IEC-6 rat intestinal crypt cells." J.Cell.Physiol.171. 284-290 (1997)

Kimura, H.等人：“细菌脂多糖降低了肠道屏障功能并改变了 IEC-6 大鼠肠隐窝细胞中 7H6 抗原的定位。”