The Analysis of Neuronal Mechanism of Vocal Nucleus related to Testosterone in Passerine.

雀形目声带核与睾酮相关的神经机制分析。

• 批准号: 11680793
• 负责人: AOKI Kiyoshi
• 金额: $ 2.37万
• 依托单位: Sophia University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680793/
• 关键词: Bengalese finches; distance call (DC); dorsomedial nucleus (DM); excitable synapse; RA nucleus; playback; impulse synapse; testosterone; distress callディストレス・コール(DC); 軸索; 非対称型シナプス; robust nucleus of archistriatum(RA核); 神経活動; destance callディスタン

The purpose of this study is to clarify the physiological function and anatomical structure of the dorsomedial nucleus (DM) in the vocal control system and in the producing of sexually different acoustic strtuctures of distance calls (DC) of instinct behavior in sexually mature Bengalese finches (Lonchura striata var. domestica) Immature males and females have acoustically similar distance calls and in sexually mature male under the effect of testosterone, sex hormone, the acoustic structures of distance calls show sexual differences. This behavioral dichotomy is due to innervation of the neural input from RA to DM when robust nucleus of the arichistriatum (RA) in the male developed sexually mature. The above findings are results of analyzing acoustic structures of distance calls before and after electrolytic lesions of the bilateral RA in sexually mature males. After bilateral RA lesioning, these males have produced distance calls acoustically similar to those of female. We studied at … More the cellular level recording of the impulses of neurons in DM by microelectrode. The response patterns of spike discharges from neurons in DM of males and females did not show sexual difference. Mean impulse number of spike discharges increased just before vocalization of distance call.We investigated microstructure of the nervous system of DM in mature male by anatomical method. The synapse of anterograde labeling with neural tracers was observed to be excitable synapse. The characteristic structure of DM in male has axonal input of nerve connection from RA to the dorsoventral side in DM and this axonal input connected the neuronal circuit related to producing of distance calls.We investigated response patterns from the results of multi-unit recording of spike discharges from neuron in DM of mature males and females when we stimulated left and right acoustic organs by the method of playback of DC.The duration of the firing spike discharges producing distance call in male is longer than that in female. Also the number of impulse frequencies in male is higher than that in female.These results suggest that axonal input of neuronal connection from the RA to DM of sexually mature male modulates the acoustic structure of its DC.We clarified by neuroethological studies that the neural input from the RA to DM was due to action of testosterone, sex hormone. Less

本研究旨在阐明性成熟孟加拉雀背内侧核（DM）在发声控制系统中的生理功能和解剖结构，以及DM在产生本能行为远距离叫声（DC）的性别差异声学结构中的作用。性成熟雄性在睾酮、性激素的作用下，远距离叫声的声学结构表现出性别差异。这种行为的二分法是由于神经支配的神经输入从RA到DM时，强大的核的arichistriatum（RA）在男性发展性成熟。上述发现是分析性成熟男性双侧RA电解损伤前后远距离呼叫的声学结构的结果。双侧RA病变后，这些男性产生的距离呼叫声学上类似于女性。我们在 ...更多信息 用微电极在细胞水平记录糖尿病神经元冲动。雄性和雌性DM神经元锋电位反应模式无性别差异。用解剖学方法研究了成年雄性DM神经系统的显微结构。神经示踪剂顺行标记的突触为可兴奋突触。雄性DM的特征性结构是RA至DM背腹侧的神经连接的轴突输入，这种轴突输入连接了与产生远距离呼叫有关的神经回路。用直流电回放法记录成年雄性和雌性DM神经元刺激左右听器时的锋电位放电，雄鼠产生远距离呼叫的放电峰长于雌鼠。这些结果表明，性成熟男性RA至DM的神经元连接的轴突输入调节了其DC的声学结构，我们从神经行为学研究中阐明了RA至DM的神经输入是由于性激素睾酮的作用。少

项目成果

Kiyoshi AOKI: "The role of dorsomedial nucleus (DM) of intercollicular complex with regard to sexual difference of distance calls in Bengalese finches."Zoological Science. 17No.9. 1231-1238 (2000)

Kiyoshi AOKI：“丘间复合体的背内侧核（DM）对于孟加拉雀远距离叫声的性别差异的作用。”动物学。

Watanabe, A.and Aoki, K.: "The role of Auditory Feedback in the Maintenance of Song in Adult Male Bengalese Finches Lonchura striata var. domestica."Zoological Science. Vol.15, No. 6. 837-841 (1998)

Watanabe, A. 和 Aoki, K.：“听觉反馈在成年雄性孟加拉雀 Lonchura striata var. Domestica 鸣叫维持中的作用。”动物学科学。

Yazaki, Y., Matsushima, T.and Aoki, K.: "Testosterone modulates stimulation-induced calling behavior in Japanese quails."J.Comp.Physiol.A.. vol.184. 13-19 (1999)

Yazaki, Y.、Matsushima, T. 和 Aoki, K.：“睾酮调节日本鹌鹑刺激诱导的叫声行为。”J.Comp.Physiol.A.. vol.184。

Kiyoshi AOKI: "Testosterone modulates stimulation-induced calling behavior in Japanese quails."J.Comp.Physiol.A.. 184. 13-19 (1999)

Kiyoshi AOKI：“睾酮调节日本鹌鹑刺激诱导的叫声行为。”J.Comp.Physiol.A.. 184. 13-19 (1999)

Kiyoshi AOKI: "Stimulation elicits the chick crowing with testosterone in Japanese quail chicks"Zoological Science. 14. 227-231 (1997)

Kiyoshi AOKI：“刺激会引起日本鹌鹑雏鸡体内睾酮的啼叫”《动物学》。