The analysis of neuronal mechanism of the nucleus in the midbrain related to calling behavior with sexual difference in birds

鸟类中脑核与性别差异叫声相关的神经机制分析

• 批准号: 13680879
• 负责人: AOKI Kiyoshi
• 金额: $ 2.11万
• 依托单位: Sophia University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680879/
• 关键词: Bengalese finches; dorsomedial nucleus(DM); sexual difference; distance call(DC); robust nucleus of the archistriatum (RA); patch clamp recording; sensory motor learning stage; after crystallization stage; distance call(DC); 音声パターン; スライス標本

The purpose of this study is to clarify the neural function and anatomical structure at the cellular level in network of the dorsomedial nucleus(DM) in the vocal control system and in the producing of sexually different acoustic structures of distance calls(DC) of instinct behavior in sexually mature Bengalese finches (Lonchura striata var. domestica) Immature males and females have acoustically similar distance calls and in sexually mature male, the acoustic structures of distance calls show sexual differences. This behavioral dichotomy is due to innervation of the neural input from RA to DM when robust nucleus of the arichistriatum (RA) in the male developed sexually mature. The above findings are results of analyzing acoustic structures of distance calls before and after electrolytic lesions of the bilateral RA in sexually mature males. After bilateral RA lesioning, these males have produced distance calls acoustically similar to those of female. We studied at the cellular level rec … More ording of the impulses of neurons in the robust nucleus of the archistriatum (RA) by cell patch clamp.In this study, whole cell patch clamp recordings were made from neurons of the slice preparation in the RA, which is a motor control nucleus of male-specific vocalization in male birds of different developmental stage. We focused on the firing patterns in the neural activity of RA neurons, and also focused on the difference in neural activity between the "sensory motor learning" stage and the "after crystallization" stage. The neurons in the RA were categorized into three types according to their electrophysiological properties under voltage clamp recording. The three types are "non-spike", "single-spike" and "multi-spike" type neurons. Distribution ratios of the three types changed during developments. We compared the distribution of the three types of these neurons in the two different learning stages. The distribution ratios of multi-spike type neuron was significantly higher than that of the single-spike type neuron in the sensory motor learning stage, and the relationship between the distribution ratios was reversed in the after crystallization stage. No significant difference between the distribution ratios of the non-spike type neuron in the two learning stages was observed Less

本研究旨在阐明性成熟孟加拉雀(Lonchura Striata Var)在发声控制系统背内侧核(Dm)网络中的神经功能和细胞水平的解剖结构，以及在产生性别不同的本能行为的长途呼叫(DC)的声学结构中的作用。未成熟的雄性和雌性在听觉上相似，而在性成熟的雄性中，长途呼叫的声音结构显示出性别差异。这种行为二分法是由于当男性的雄核发育成熟性成熟时，从RA到DM的神经输入的神经支配。以上结果是对性成熟男性双侧RA电解损毁前后远距离呼叫声结构的分析结果。在双侧RA损毁后，这些雄性发出与雌性相似的远距离叫声。我们在细胞水平上研究了rec…本研究采用全细胞膜片钳技术，对雄性雏鸟雄鸟发声的运动控制核团--粗大核团(RA)的神经元进行全细胞膜片钳记录。我们重点研究了RA神经元神经活动的放电模式，并重点研究了感觉运动学习阶段和结晶后阶段神经活动的差异。在电压钳记录下，视网膜区神经元根据电生理特性分为三种类型。这三种类型的神经元分别为“非棘型”、“单棘型”和“多棘型”神经元。这三种类型的分配比在开发过程中发生了变化。我们比较了这三种类型神经元在两个不同学习阶段的分布。在感觉运动学习阶段，多棘型神经元的分布比例显著高于单棘型神经元，而在结晶后期，两者的分布比例关系相反。非棘型神经元在两个学习阶段的分布比例没有显著差异

项目成果

Fukushima Y, Takagi H, Aoki K: "Developmental difference of neural functions in the song and call control system in Bengalese finches"Neuroscience Letters. 334/2. 70-74 (2002)

Fukushima Y、Takagi H、Aoki K：“孟加拉雀鸣叫和呼叫控制系统中神经功能的发育差异”《神经科学快报》。

Fukushima, Y., Aoki, K.: "Neural function of the mesencephalic dorsomedial nucleus (DM) on distance call production in Bengalese finches"Zoological Science. 19/4. 393-402 (2002)

Fukushima，Y.，Aoki，K.：“中脑背内侧核（DM）对孟加拉雀远距离呼叫产生的神经功能”动物学。

Y. Fukushima, K. Aoki: "Neural function of the mesencephalic dorsomedial nucleus (DM) on distance call production in Bengalese finches"Zoological Science. Vol.19, No.4. (2002)

Y. Fukushima，K. Aoki：“中脑背内侧核（DM）对孟加拉雀远距离叫声产生的神经功能”动物学。

Fukushima, Y., and Aoki, K.: "Neural function of the mesencephalic dorsomedial nucleus (DM) on distance call production in Bengalese finches"Zoological Science. Vol.19, No.4. 393-402 (2002)

Fukushima, Y. 和 Aoki, K.：“中脑背内侧核 (DM) 对孟加拉雀远距离叫声产生的神经功能”动物学。

Fukushima, Y., Takagi, H., and Aoki, K.: "Developmental difference of neural functions in the song and call control system in Bengalese finches"Neuroscience Letters. Vol. 334, No.2. 70-74 (2002)

Fukushima, Y.、Takagi, H. 和 Aoki, K.：“孟加拉雀鸣叫和呼叫控制系统中神经功能的发育差异”神经科学快报。