The analysis of neuronal mechanism of the nucleus in the midbrain related to calling behavior with sexual difference in birds

本研究旨在从细胞水平上阐明性成熟孟加拉雀（Lonchura striata var.未成熟的雄鸟和雌鸟的远距离叫声在声学结构上具有相似性，而在性成熟的雄鸟中，远距离叫声的声学结构表现出性别差异。这种行为的二分法是由于神经支配的神经输入从RA到DM时，强大的核的arichistriatum（RA）在男性发展性成熟。上述发现是分析性成熟男性双侧RA电解损伤前后远距离呼叫的声学结构的结果。双侧RA病变后，这些男性产生的距离呼叫声学上类似于女性。我们在细胞水平上研究了 ...更多信息 本研究采用全细胞膜片钳技术记录了不同发育阶段雄性鸟类原纹状体强健核（Robustnucleusofthearchistriatum，RA）神经元的冲动。我们集中在RA神经元的神经活动的放电模式，也集中在“感觉运动学习”阶段和“结晶后”阶段之间的神经活动的差异。根据电压钳记录的电生理特性，将RA内神经元分为三类。这三种类型是“无锋电位”、“单锋电位”和“多锋电位”型神经元。三种类型的分布比例随发育而变化。我们比较了这三种类型的神经元在两个不同的学习阶段的分布。在感觉运动学习阶段，多棘波型神经元的分布比例显著高于单棘波型神经元，而在结晶后阶段，两者的分布比例呈相反的关系。非锋电位型神经元在两个学习阶段的分布比例无显著性差异

The purpose of this study is to clarify the neural function and anatomical structure at the cellular level in network of the dorsomedial nucleus(DM) in the vocal control system and in the producing of sexually different acoustic structures of distance calls(DC) of instinct behavior in sexually mature Bengalese finches (Lonchura striata var. domestica) Immature males and females have acoustically similar distance calls and in sexually mature male, the acoustic structures of distance calls show sexual differences. This behavioral dichotomy is due to innervation of the neural input from RA to DM when robust nucleus of the arichistriatum (RA) in the male developed sexually mature. The above findings are results of analyzing acoustic structures of distance calls before and after electrolytic lesions of the bilateral RA in sexually mature males. After bilateral RA lesioning, these males have produced distance calls acoustically similar to those of female. We studied at the cellular level rec … More ording of the impulses of neurons in the robust nucleus of the archistriatum (RA) by cell patch clamp.In this study, whole cell patch clamp recordings were made from neurons of the slice preparation in the RA, which is a motor control nucleus of male-specific vocalization in male birds of different developmental stage. We focused on the firing patterns in the neural activity of RA neurons, and also focused on the difference in neural activity between the "sensory motor learning" stage and the "after crystallization" stage. The neurons in the RA were categorized into three types according to their electrophysiological properties under voltage clamp recording. The three types are "non-spike", "single-spike" and "multi-spike" type neurons. Distribution ratios of the three types changed during developments. We compared the distribution of the three types of these neurons in the two different learning stages. The distribution ratios of multi-spike type neuron was significantly higher than that of the single-spike type neuron in the sensory motor learning stage, and the relationship between the distribution ratios was reversed in the after crystallization stage. No significant difference between the distribution ratios of the non-spike type neuron in the two learning stages was observed Less