Development of highiy bioactive artificial bone by bioactive material anci growth factor

利用生物活性材料和生长因子开发高生物活性人工骨

• 批准号: 13671505
• 负责人: NEO Masashi
• 金额: $ 1.98万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671505/
• 关键词: Artificial bone; Growth factor; Hydroxyapatite; Bioactivity; Porous; D-FGF; Bone formation; 生体活性セラミック; マイクロCT; 血管新生; バイオミメティック法; 生体活性材料

The purpose of this study is to create an artificial bone that has a comparable bone-forming ability to natural bone by adding growth factors to bioactive ceramics1: Enhancement of bone-bonding ability by TAK-778TAK778 is a newly formed low molecular agent, which may exert regulatory effects on osteoblast differentiation. This agent was applied to the interface between bioactive ceramics and bone at implantation, which demonstrated that TAK-778 enhanced the bone bonding strength of the ceramics2: Absorption of porous AWCylinders of porous AW or porous HA (porosity 70% and mean pore diameter 200 urn for both materials) were implanted into the bone marrow cavity of rabbit femurs, and analyzed chronologically. AW was absorbed continuously, and at 12 months the residual material corresponded to about 30% of the starting material, whereas HA was hardly absorbed. The volume of newly formed bone in the material pores was higher with AW than with HA. AW-bone bonding was maintained during bone remodeling and material absorption. Thus porous AW showed faster absorption and greater bone ingrowth than HA, suggesting better suitability for a carrier of growth factors3: Boneforming ability of AW-b-FGF complexPorous AW cylinders were soaked in the solution of b-FGF under high vacuum and were implanted into rabbit femur. Although more vascular formation into the pores was observed in FGF group than control (AW without b-FGF), the amounts of bone formation were comparable

本研究的目的是通过向生物活性陶瓷中添加生长因子来创建具有与天然骨相当的骨形成能力的人工骨1：TAK-778的骨结合能力增强TAK-778是一种新形成的低分子试剂，可能对成骨细胞分化产生调节作用。将该试剂应用于植入时生物活性陶瓷与骨之间的界面，这证明TAK-778增强了陶瓷的骨结合强度2：多孔AW或多孔HA的多孔AWD的吸收（两种材料的孔隙率均为70%，平均孔径均为200 μ m）植入兔股骨的骨髓腔中，并按时间顺序进行分析。AW被持续吸收，在12个月时，残留材料相当于起始材料的约30%，而HA几乎不被吸收。AW材料孔隙中新形成的骨体积高于HA。在骨重建和材料吸收过程中，AW-骨结合得以维持。因此，多孔AW比HA表现出更快的吸收和更大的骨长入，表明更适合作为生长因子的载体3：AW-b-FGF复合物的成骨能力将多孔AW圆柱体在高真空下浸泡在b-FGF溶液中，并植入兔股骨中。虽然FGF组比对照组（不含b-FGF的AW）观察到更多的血管形成，但骨形成量相当

项目成果

K.Ohsawa: "Resorption of porous A-W Glass Ceramics"Bioceramics. 14. 217-220 (2001)

K.Ohsawa：“多孔 A-W 玻璃陶瓷的吸收”生物陶瓷。

H.KATO: "Bone bouding in sintered hydrcxyapatite combined with anew synthe sized agent, TAK-778"J. Bioined Mater. Res.. 54. 619-629 (2001)

H.KATO：“烧结羟基磷灰石中的骨结合与新型合成剂 TAK-778 相结合”J。