Development of a Hybrid Artificial Liver for Treatment of Acute and Chronic Liver Failure Patients

开发用于治疗急性和慢性肝衰竭患者的混合人工肝

基本信息

  • 批准号:
    12305053
  • 负责人:
  • 金额:
    $ 24.76万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2001
  • 项目状态:
    已结题

项目摘要

1. Development of a hybrid artificial liver for clinical trialWe developed a hybrid artificial liver (HAL) which consists of a hepatocyte culture module with multi-capillary polyurethane foam packed-bed containing 200 g primary porcine hepatocytes and an extracorporeal circulation line. Almost all of the hepatocytes in the module formed spheroids, and they maintained the liver specific functions for at least 2 weeks. As preclinical study, the HAL was applied to about 25 kg warm ischemic liver failure pigs. In the preclinical study, we demonstrated its effectiveness such functions as suppression of increase of blood ammonia, maintenance of blood glucose, stabilization of vital sign and urinary excretion, extension of survival time. The HAL was effective to stabilize the general conditions of the body in addition to supporting various liver functions. These results suggest that the HAL has a strong possibility to be used in treating acute liver failure patients.2. Development of novel hybrid artificial liver module using hepatocyte organoid cultureAs a novel artificial liver module, we developed a hollow fiber module having a liver lobule-like structure, in which the hollow fibers representing blood vessels are arranged by spacers in a micro-regular arrangement within 150 μm. Inoculated hepatocytes in the extra-fiber space were formed in a lotus root-organoid by loading appropriate centrifugal force. The novel module achieved high cell density of about 4.0 x l0^7 cells / cm^3 - module and maintained better liver function compared to previous hollow fiber modules for several month. These results suggest that this novel module will be a new HAL for treatment of both chronic and acute liver failure patients, and for long-term bridge use for liver transplantation. Now we are investigating the optimization and scale up of the module.
1.用于临床试验的混合型人工肝的研制我们研制了一种混合型人工肝,它由含有200g原代猪肝细胞的多毛细管聚氨酯泡沫填充床和一条体外循环线组成。该模块中几乎所有的肝细胞都形成了球形,它们至少维持了2周的肝脏特异性功能。作为临床前研究,HAL应用于约25公斤热缺血性肝衰竭猪。在临床前研究中,我们证实了其具有抑制血氨升高、维持血糖、稳定生命体征和尿液排泄、延长生存时间等作用。HAL除了支持各种肝功能外,还能有效地稳定身体的一般状况。这些结果提示HAL有很强的可能性用于治疗急性肝功能衰竭患者。新型混合型人工肝模块的研制作为一种新型人工肝模块,我们研制了一种具有肝小叶样结构的中空纤维组件,其中代表血管的中空纤维在150μm内以微规则的方式排列,通过加载适当的离心力在纤维外间隙内形成接种的肝细胞。与以前的中空纤维组件相比,这种新型组件的细胞密度高达约4.0×10~(-7)个/厘米~3-模块,并保持了更好的肝功能。这些结果表明,这种新的模块将是一种新的HAL,用于治疗慢性和急性肝衰竭患者,并用于肝移植的长期过渡性使用。现在我们正在研究该模块的优化和放大。

项目成果

期刊论文数量(29)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
中澤浩二: "PUF/スフェロイド人工肝の倫理委員会申請と問題点"組織培養工学. 27(4). 148-151 (2001)
Koji Nakazawa:“PUF/球体人工肝伦理委员会的申请和问题”组织培养工程 148-151 (2001)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
K.FUNATSU: "Hybrid Artificial Liver Using Hepatocyte Organoid Culture"Artificial Organs. 25巻(in press). (2001)
K.FUNATSU:“使用肝细胞类器官培养的混合人工肝脏”人工器官第 25 卷(印刷中)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
H.Iijima: "Development of a hybrid artificial liver support system and preclinical animal experiments"Journal of Artificial Organs. 3(2). 112-116 (2000)
H.Iijima:“混合人工肝支持系统的开发和临床前动物实验”人工器官杂志。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Y.Fujii: "Intensive promotion of spheroid formation by soluble factors in a hepatocyte-condition medium"Journal of Biomaterials Science Polymer Edition. 17(7). 731-745 (2000)
Y.Fujii:“肝细胞条件培养基中可溶性因子对球体形成的强烈促进”生物材料科学杂志聚合物版。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
J.Fukuda: "Mass preparation of primary porcine hepatocytes and the design of a hybrid artificial liver module using spheroid culture for a clinicaltrial"The International Journal of Artificial Organs. 24(11). 799-806 (2001)
J.Fukuda:“原代猪肝细胞的大量制备以及使用球状培养物进行临床试验的混合人工肝模块的设计”《国际人工器官杂志》。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

FUNATSU Kazumori其他文献

FUNATSU Kazumori的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('FUNATSU Kazumori', 18)}}的其他基金

Development of a Hybrid Artificial Liver for Treatment of Chronic Liver Failure Patients
开发用于治疗慢性肝衰竭患者的混合人工肝
  • 批准号:
    14205119
  • 财政年份:
    2002
  • 资助金额:
    $ 24.76万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Development of the Numerical Simulation Codes of the Non-isothermal Polymer Processing Containing Melting. Mixing and Kneading Processes That Are Important for the Finished Morphology or Properties of Polymer Products
包含熔融的非等温聚合物加工数值模拟代码的开发。
  • 批准号:
    11555201
  • 财政年份:
    1999
  • 资助金额:
    $ 24.76万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
Development of Hybrid Artificial Liver for Clinical Treatment of Fulminant Hepatic Failure Patient
混合型人工肝的开发用于临床治疗暴发性肝衰竭患者
  • 批准号:
    10305062
  • 财政年份:
    1998
  • 资助金额:
    $ 24.76万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Development on the Numerical Simulation Technique for General Purpose of Three-dimensional Non-Newtonian Pure Viscous and Viscoelastic Flows
三维非牛顿纯粘弹性流通用数值模拟技术的发展
  • 批准号:
    08455359
  • 财政年份:
    1996
  • 资助金额:
    $ 24.76万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of high performance hybrid artificial liver and its evaluation in preclinical test
高性能混合型人工肝的研制及其临床前评价
  • 批准号:
    08555206
  • 财政年份:
    1996
  • 资助金额:
    $ 24.76万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Evaluation of the performance of an hybrid artifical liver using three-dimensional culture of hepatocytes by hepatic failure small animals
肝衰竭小动物肝细胞三维培养杂交人工肝性能评价
  • 批准号:
    06453107
  • 财政年份:
    1994
  • 资助金额:
    $ 24.76万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Development of an hybrid artificial liver for human medical use equipped with the newest regulation system for control of culture environment of hepatocytes
开发配备最新肝细胞培养环境控制系统的医用混合人工肝
  • 批准号:
    06555248
  • 财政年份:
    1994
  • 资助金额:
    $ 24.76万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
Development of a hybrid artifical liver using PUF/three dimensional culture of normal hepatocytes
利用 PUF/正常肝细胞三维培养开发混合型人工肝
  • 批准号:
    04555225
  • 财政年份:
    1992
  • 资助金额:
    $ 24.76万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
Engineering research on the production of useful biologicals by mass culture of animal and plant cells
动植物细胞大量培养生产有用生物制品的工程研究
  • 批准号:
    02453113
  • 财政年份:
    1990
  • 资助金额:
    $ 24.76万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Research on mass culture of cell from the viewpoint of chemical engineering
从化学工程的角度进行细胞大量培养的研究
  • 批准号:
    62550705
  • 财政年份:
    1987
  • 资助金额:
    $ 24.76万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似海外基金

PLAG1 and HMGA2 Gene Rearrangements in Salivary duct Carcinoma - Preclinical Study of Novel Molecular Target Therapy -.
唾液腺管癌中的 PLAG1 和 HMGA2 基因重排 - 新型分子靶向治疗的临床前研究 -。
  • 批准号:
    22K09938
  • 财政年份:
    2022
  • 资助金额:
    $ 24.76万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
A Preclinical Study Evaluating and Comparing the Efficacy of Tissue Engineered Vascular Grafts to Polytetrafluoroethylene Grafts
评估和比较组织工程血管移植物与聚四氟乙烯移植物功效的临床前研究
  • 批准号:
    10420151
  • 财政年份:
    2022
  • 资助金额:
    $ 24.76万
  • 项目类别:
A Preclinical Study Evaluating and Comparing the Efficacy of Tissue Engineered Vascular Grafts to Polytetrafluoroethylene Grafts
评估和比较组织工程血管移植物与聚四氟乙烯移植物功效的临床前研究
  • 批准号:
    10645005
  • 财政年份:
    2022
  • 资助金额:
    $ 24.76万
  • 项目类别:
Preclinical study to develop treatment strategies against KRAS-mutated non-small cell lung cancer
开发针对 KRAS 突变非小细胞肺癌治疗策略的临床前研究
  • 批准号:
    22K08237
  • 财政年份:
    2022
  • 资助金额:
    $ 24.76万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Preclinical study of deep brain stimulation of the nucleus accumbens for the treatment of severe self-injurious behaviours associated with Autism Spectrum Disorder
伏隔核深部脑刺激治疗与自闭症谱系障碍相关的严重自残行为的临床前研究
  • 批准号:
    466667
  • 财政年份:
    2021
  • 资助金额:
    $ 24.76万
  • 项目类别:
    Studentship Programs
Preclinical study of regenerative therapy for bone defects using dental pulp stem cells
牙髓干细胞再生治疗骨缺损的临床前研究
  • 批准号:
    20K10017
  • 财政年份:
    2020
  • 资助金额:
    $ 24.76万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Preclinical study of near-infrared photoimmunotherapy for pancreatic cancer that incorporates nanoparticulate TLR9 agonist K3-SPG
结合纳米颗粒 TLR9 激动剂 K3-SPG 的近红外光免疫疗法治疗胰腺癌的临床前研究
  • 批准号:
    20K08329
  • 财政年份:
    2020
  • 资助金额:
    $ 24.76万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Preclinical study of photoimmunotherapy for tumor microenvironment
光免疫治疗肿瘤微环境的临床前研究
  • 批准号:
    20K09009
  • 财政年份:
    2020
  • 资助金额:
    $ 24.76万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Preclinical study on organ preconditioning before transplantation for marginal grafts
边缘移植物移植前器官预处理的临床前研究
  • 批准号:
    19K09722
  • 财政年份:
    2019
  • 资助金额:
    $ 24.76万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Novel preclinical study of psychiatric disorders using plasmalogen
使用缩醛磷脂进行精神疾病的新型临床前研究
  • 批准号:
    19K17125
  • 财政年份:
    2019
  • 资助金额:
    $ 24.76万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了