Molecular-biological analysis of function of thylakoid lipids in photosynthesis

类囊体脂质在光合作用中功能的分子生物学分析

• 批准号: 12640635
• 负责人: WADA Hajime
• 金额: $ 2.37万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12640635/
• 关键词: Photosynthesis; Cyanobacteria; Thylakoid membrane; Phosphatidylglyceral; Blue-green alage; Higher plant; Arabidopsis thaliana; シアノドクテリア

Thylakoid membranes in chloroplasts of eukaryotic plants and cyanobacterial cells are the sites of the primary processes of oxygenic photosynthesis. The lipid composition of thylakoid membranes is highly conserved among eukaryotic plants and cyanobacterial strains, and is distinct from that of other membranes, which contain phospholipids as the major glycerolipids. As glycerolipids thylakoid membranes contain glycolipids, monogalactosyldiacylglycerol, digalactosyldiacylglycerol and sulfoquinovosyldiacylglycerol, and a phospholipid, phosphatidylglycerol (PG). To understand the function of glycerolipids in photosynthesis in vivo, it is important to identify the genes involved in the- biosynthesis of glyceroiipids and to manipulate the composition of glycerolipids of thylakoid membranes.In this study, we have identified a gene of Synechocystis sp. PCC6803, which encodes PG phosphate synthase required for the biosynthesis of PG that is the only phospholipid in thylakoid membranes, and inactivated the gene to create a mutant that is not able to synthesize PG. The results obtained by characterization of the mutant clearly demonstrated that PG is essential for growth of Synechocystis sp. PCC6803 and an indispensable component in the maintenance of photosystem II reaction center.

真核植物和蓝藻细胞叶绿体中的类囊体膜是含氧光合作用初级过程的场所。类囊体膜的脂质组成在真核植物和蓝藻菌株中高度保守，与其他膜不同，后者含有磷脂作为主要甘油脂。作为甘油脂，类囊体膜含有糖脂、单半乳糖基二酰基甘油、二半乳糖基二酰基甘油和磺基奎诺基二酰基甘油，以及磷脂、磷脂酰甘油(PG)。为了了解甘油脂在体内光合作用中的功能，重要的是鉴定参与甘油脂生物合成的基因并操纵类囊体膜的甘油脂的组成。在这项研究中，我们鉴定了集胞藻属的一个基因。 PCC6803编码类囊体膜中唯一的磷脂PG生物合成所需的PG磷酸合酶，并使该基因失活以产生不能合成PG的突变体。通过突变体的表征获得的结果清楚地表明PG对于集胞蓝藻的生长是必需的。 PCC6803是维持光系统II反应中心不可缺少的成分。

项目成果

Miki Hagio: "Direct evidence for requirement of phosphatidylglycerol in photosystem II of photosynthesis"Plant Physiology. 124. 795-804 (2000)

Miki Hagio：“光合作用的光系统 II 需要磷脂酰甘油的直接证据”植物生理学。

Xu, C., Hartel, H., Wada, H., Hagio, M., Yu B., Eakin, C. and Banning, C.: "The pgp1 mutant locus of Arabidopsis encodes a phosphatidylglycerolphosphate synthase with impaired activity"Plant Physiol.. (in press). (2002)

Xu, C.、Hartel, H.、Wada, H.、Hagio, M.、Yu B.、Eakin, C. 和 Banning, C.：“拟南芥的 pgp1 突变位点编码活性受损的磷脂酰甘油磷酸合酶”植物

Changcheng Xu: "The pgpl mutant locus of Arabidopsis encodes a phosphatidylglycerol phosphate synthare with impaired activity"Plant Physiology. (2002)

徐长城：“拟南芥的 pgpl 突变位点编码活性受损的磷脂酰甘油磷酸合成酶”《植物生理学》。

Gombos, Z., Varkconyi., Z., Hagio, M., Iwaki, M., Kovacs, L., Masamoto, K., Itoh, S. and Wada, H.: "Phosphatidylglycerol requirement for the funtion of electron acceptor plastoquinone Q_B in the photosystem II reaction center"Biochemistry. (in press). (20

Gombos, Z.、Varkconyi., Z.、Hagio, M.、Iwaki, M.、Kovacs, L.、Masamoto, K.、Itoh, S. 和 Wada, H.：“电子受体功能所需的磷脂酰甘油

Zoltan Gombos: "Phosphatidylglycerol requirement for the function of electron acceptor plastquinone QB in the photosystem II reaction center"Biochemistry. (in press). (2002)

Zoltan Gombos：“光系统 II 反应中心电子受体质体醌 QB 功能所需的磷脂酰甘油”生物化学。