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Moleculaar-biological analysis of function of chloroplast membrane lopids in highr plants

高等植物叶绿体膜脂质功能的分子生物学分析

基本信息

批准号：
14540596
负责人：
WADA Hajime
金额：
$ 1.98万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14540596/
关键词：
Photosynthesis Arabidopsis thaliana thylakoid membane Phosphatidylglycerol chloroplast Cyanabacteria

项目摘要

Phosphatidylglycerol (PG) is a ubiquitous phospholipid in the biological membranes of many organisms. In plants, PG is mainly present in chloroplasts and has been suggested to play specific roles in photosynthesis. However, the specific roles of PG in chloroplasts have not been well understood. In this study, we have investigated the roles of PG in chloroplasts with two T-DNA tagged lines of Arabidopsis thaliana that have a T-DNA insertion in the PGP1 gene encoding a PG phosphate synthase involved in the biosynthesis of PG in chloroplasts. In homozygous plants of the T-DNA tagged lines, the PGP1 gene was completely disrupted. The growth of these knockout mutants was dependent on the presence of sucrose in the growth medium, and these plants had pale yellow-green leaves. The leaves of the mutants had remarkably large intercellular spaces due to the reduction in the number of mesophyll cells. The development of chloroplasts in the leaf cells was severely arrested in the mutants. Mesophyll cells with chloroplast particles are only found around vascular structures, whereas epidermal cells are enlarged but largely conserved. The content of PG in ghe mutants was reduced to 12% of the of the wild type. These results demonstrate that pg is essential for the development of thylakoid membranes In A.thaliana.

磷脂酰甘油（PG）是一种在许多生物膜中普遍存在的磷脂。在植物中，PG主要存在于叶绿体中，并被认为在光合作用中起特定的作用。然而，PG在叶绿体中的具体作用尚不清楚。在这项研究中，我们用两个T-DNA标记的拟南芥细胞系研究了PG在叶绿体中的作用，这两个细胞系的T-DNA插入了编码PG磷酸合酶的ppgp1基因，该基因参与了PG在叶绿体中的生物合成。在T-DNA标记系的纯合子植株中，ppg1基因被完全破坏。这些基因敲除突变体的生长依赖于生长培养基中蔗糖的存在，这些植物的叶子呈淡黄绿色。由于叶肉细胞数量的减少，突变体叶片的细胞间隙明显增大。突变体的叶片细胞叶绿体发育严重受阻。带有叶绿体颗粒的叶肉细胞仅在维管结构周围发现，而表皮细胞则较大，但大部分保守。突变体中PG的含量降至野生型的12%。这些结果表明pg对拟南芥类囊体膜的发育至关重要。