Manipulation of mitochondria and chloroplast genome organization by artificially-synthesized chimera

通过人工合成的嵌合体操纵线粒体和叶绿体基因组组织

基本信息

  • 批准号:
    12660004
  • 负责人:
  • 金额:
    $ 2.3万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2002
  • 项目状态:
    已结题

项目摘要

My objective was to (1) make clear the heteroplasmic variation and shuft from normal mitochondria to CMS one by chimera formation and maintainance. (2) For that, genome structure of organelle must be nade clear, especially for mitcondria with chloroplast. (3) Base on the results of (1) and (2), controlling mechanism of the induction of CMS by chimera synthesis.As the results, RFLP patterns probed with known mitochodra and chloroplast probes were almost corresponded with that 〓radish CMS that has indicator orf138. By using the indicator orf138, three has heroplasimic sequnenses could be identified. Totally 99% of those sequences that were extended 3kb 3' direction from orf 138 were the same as that of CMS radish (by Maccolof).However, chloroplast bases based on sequence is not identitifoed. Full map or sequence could not be identified, yet. However, general ssituation that has been shited from normal genome molecule(s) to CMS types in the both organella is very clear without inter-molecular recombination.Those results were just the same as in intergeneric cell fusion hybrid between radish and cabbage. From our comparable results, it will be possible to induce and control CMS quantitative and qualitative genome constraction by tissue culture, chimera synthesis etc. Further the mechanism research should originate new CMS and hybrid breeding system
我的目标是(1)通过嵌合体的形成和维持来明确异质性变异和从正常线粒体到 CMS 的转变。 (2)为此,细胞器的基因组结构必须清楚,尤其是具有叶绿体的线粒体。 (3)基于(1)和(2)的结果,嵌合体合成诱导CMS的控制机制。 结果,用已知线粒体和叶绿体探针探测的RFLP模式与具有指示剂orf138的〓萝卜CMS几乎一致。通过使用orf138指示剂,可以鉴定出三个具有英雄形体的序列。从 orf 138 向 3' 方向延伸 3kb 的序列中,总共 99% 与 CMS 萝卜 (Maccolof) 的序列相同。然而,基于序列的叶绿体碱基尚未确定。目前还无法识别完整的图谱或序列。然而,两种细胞器中从正常基因组分子到CMS类型的一般情况是非常清楚的,没有分子间重组。这些结果与萝卜和甘蓝之间的属间细胞融合杂种相同。从我们的可比结果来看,通过组织培养、嵌合体合成等方法诱导和控制CMS定量和定性基因组构建将成为可能。进一步的机制研究应开创新的CMS和杂交育种系统

项目成果

期刊论文数量(49)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
肖秋濱, 陳 莉平, 保木祐樹, 栗田樹, 野口貴, 茂木岳, 平田豊: "ダィコンとキャベツとの属間キメラの栄養繁殖世代で認められた新しいタイプの出現とその特料"育種研究. 4. 59-66 (2002)
Qiubin肖,Liping Chen,Yuki Hoki,Itsuki Kurita,Takashi Noguchi,Takeshi Mogi,Yutaka Hirata:“萝卜和卷心菜之间的属间嵌合体无性繁殖一代中认识到新类型的出现及其特殊特征”育种研究4. 59 -66 (2002)
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    0
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Thanh, V.C., Hirata, Y.: "Seed storage protein diversity of three rice species in the Mekong Delta.4:59-67.(2002)"Biosphere Conservation. 4. 59-77 (2003)
Thanh, V.C., Hirata, Y.:“湄公河三角洲三种水稻的种子储存蛋白多样性。4:59-67。(2002)”生物圈保护。
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    0
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Hirata, Y.: "Molecukar mechanism of graft transformation in red pepper (Capsicum annuum L.)"Acta Hort.. 5(in press). (2003)
Hirata, Y.:“红辣椒(Capsicum annuum L.)嫁接转化的分子机制”Acta Hort.. 5(印刷中)。
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    0
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H-T Kin, D-Y Shin, H-S Hwang, J-Hwang, Y-U Shin, Y.Hirata, Ill-S.: "Determination of S-genotypes of pear(Pyrus pulifolia) cultivars by S-RNase sequensing and PCR-RFLP"Mol.Cells. (2002)
H-T Kin、D-Y Shin、H-S Hwang、J-Hwang、Y-U Shin、Y.Hirata、Ill-S.:“通过 S-RNase 测序和 PCR-RFLP 测定梨(Pyrus pulifolia)品种的 S 基因型”Mol。
  • DOI:
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    0
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Zhou, J. et al.: "Interactions between different gentypic tissue in atrus graft chimera."Fuplytica. (in press). (2002)
Zhou,J.等人:“阿特鲁斯移植嵌合体中不同基因型组织之间的相互作用。”Fuplytica。
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    0
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HIRATA Yutaka其他文献

HIRATA Yutaka的其他文献

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{{ truncateString('HIRATA Yutaka', 18)}}的其他基金

Understanding and engineering application of cerebellar neuronal network realizing predictive adaptive control
实现预测自适应控制的小脑神经网络的理解及工程应用
  • 批准号:
    16H02901
  • 财政年份:
    2016
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Understanding roles of the cerebellar-brainstem loop in adaptive motor control and application to real world robot control
了解小脑脑干环路在自适应运动控制中的作用及其在现实世界机器人控制中的应用
  • 批准号:
    24300115
  • 财政年份:
    2012
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
The elucidation of central chemosensitive respiratory regulation by TRP channels expressed in glial cell.
阐明神经胶质细胞中表达的 TRP 通道对中枢化学敏感呼吸调节的作用。
  • 批准号:
    23590292
  • 财政年份:
    2011
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The elucidation of molecular basis on mechanisms for chemosensitive respiratory regulation
化学敏感性呼吸调节机制的分子基础阐明
  • 批准号:
    21790231
  • 财政年份:
    2009
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Understanding the cerebellar information processing for motor learning and application to real world motor control.
了解运动学习的小脑信息处理及其在现实世界运动控制中的应用。
  • 批准号:
    21500298
  • 财政年份:
    2009
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The elucidation of mechanisms for central chemoreceptor
中枢化学感受器机制的阐明
  • 批准号:
    19790182
  • 财政年份:
    2007
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Study on the cerebellar information processing that achieves motor learning
实现运动学习的小脑信息处理研究
  • 批准号:
    18500231
  • 财政年份:
    2006
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Mitocohdrial structure and function analysis of Ogura-type of CMS obtained in the progeny derived from interspecific chimera
种间嵌合体后代小仓型CMS的线粒体结构和功能分析
  • 批准号:
    15580004
  • 财政年份:
    2003
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Direct introduction of foreign gene in higher plants
高等植物中直接导入外源基因
  • 批准号:
    62560005
  • 财政年份:
    1987
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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十字花科种间不亲和性和自交不亲和性的综合理解
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