Chemical disease-resistance mechanism during seedling stage

苗期化学抗病机制

• 批准号: 12660104
• 负责人: SUZUKI Yoshikatsu
• 金额: $ 2.18万
• 依托单位: RIKEN INSTITUTE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660104/
• 关键词: Disease-resistant mechanism; rice; 5-alkylresorcinol

The main aim of this science program is to understand the production and metabolisms as well as disease-resistant mechanism of 5-alkylresorcinols as phytoanticipins during rice seedling stage.1. Disease-resistance effects of ARs : In vitro conidia-germination and mycelia-growth tests using rice-blight disease fungus (Magnaporthe grisea) and an in vivo infection test suggested that ARs play a some role on disease-resistant mechanism as a phytoanticipin.2. ARs metabolisms : New two metabolites (AR-A8 and AR-A10) of ARs were isolated from etiolated rice-seedlings that were irradiated for 2 days, and their structures determined based on GC-MS and ^1H-NMR spectra. These metabolites seemed to be produced via oxidative double-bond cleavage of unsaturated carbon-chains in ARs (AR15 : 1 , AR17 : 1 s, AR17 : 2). From the finding of AR-A10 metabolite, a new homolog of AR17 : 1 , 5-(10'-heptadecenyl) resorcinol was identified.3. Classification of rice varieties based on AR-homolog composition : AR-homo log composition and their amounts were examined for 13 varieties of Japonica-type rices. The homolog compositions had a close relationship to disease- resistant-gene types. Especially, the composition of 3 varieties that produce a trace amount of ARs largely different from those with the other domestic- and indica-type genes.4. ARs biosynthesis : In efficient 5-alkylresorcinol-producing, etiolated rice seedlings, feedings of odd-number fatty acid ester substrates increased only the homologs expected from the predicted ARs biosynthetic pathway. In addition, the dodecyl homolog that was biosynthesized from [^<13>C]-labeled substrate obviously showed that fatty acid unit acted as a direct precursor and formed the side-chain moiety of 5-alkylresorcinol via the predicted biosynthesis pathway.

本研究的主要目的是了解5-烷基间苯二酚类植物预生物素在水稻苗期的产生、代谢及抗病机理. AR的抗病作用：离体稻瘟病菌孢子萌发和菌丝生长试验以及体内侵染试验表明，AR作为一种植物预测因子在水稻抗病机制中发挥一定作用. AR代谢：从水稻黄化幼苗中分离到两个新的AR代谢产物AR-A8和AR-A10，并通过GC-MS和~ 1H-NMR谱确定了其结构。这些代谢物似乎是通过AR中不饱和碳链的氧化双键裂解产生的（AR 15：1、AR 17：1 s、AR 17：2）。从AR-A10代谢产物的发现中，鉴定出一个新的AR 17同系物：1，5-（10 '-十七碳烯基）间苯二酚.水稻品种的AR-同源物分类：对13个粳稻品种的AR-同源物组成及其数量进行了研究。同源物组成与抗病基因类型密切相关。特别是3个产生微量AR基因的品种，其基因组成与其它的籼型和家用基因有很大的不同. AR生物合成：在有效的5-alkylresorcinol生产，黄化水稻幼苗，饲料奇数脂肪酸酯底物增加，只有同系物预期从预测的AR生物合成途径。此外，以[^ C]标记底物生物合成的十二烷基同系物<13>明显表明，脂肪酸单元作为直接前体，通过预测的生物合成途径形成5-烷基间苯二酚的侧链部分。

项目成果

Y. Esumi, Y. Suzuki, Y. Itoh , M. Uramoto, K. Kimura, M. Yoshihama, T. Ichikawa: "Propeptin, a new inhibitor of prolyl endopeptidase produced by Microbispora II. Determination of chemical structure"J. Antibiotic. 55 (3). 296-300 (2002)

Y. Esumi，Y. Suzuki，Y. Itoh，M. Uramoto，K. Kimura，M. Yoshihama，T. Ichikawa：“Propeptin，小双孢菌 II 产生的脯氨酰内肽酶的新型抑制剂。化学结构的测定”J。

Y.Esumi, Y.Suzuki, T.Ichikawa, Y.Ikeda, K.Kimura: "SNF-4794: New enzyme inhibitors produced by Chaetomella sp"J.Anitbiotic. 56・2. 169-172 (2003)

Y.Esumi、Y.Suzuki、T.Ichikawa、Y.Ikeda、K.Kimura：“SNF-4794：由毛壳菌产生的新酶抑制剂”J.Anitbiotic 56·2（2003）。

R. Zarnowski, Y. Suzuki, Y. Esumi, and S. J. Pietr: "5-n-Alkylresorcinols from the green microalga Apatococcus constipatus"Phytochemistry. 55. 975-977 (2000)

R. Zarnowski、Y. Suzuki、Y. Esumi 和 S. J. Pietr：“来自绿色微藻 Apatococcus constipatus 的 5-n-烷基间苯二酚”植物化学。

R.Zarnowski, Y.Suzuki, I.Yamaguchi, S.J.Pietr: "Alkylresorcinols in barley (Hordeum vulgare L. distichon)grains"Z.Naturforsch. 57C. 57-62 (2002)

R.Zarnowski、Y.Suzuki、I.Yamaguchi、S.J.Pietr：“大麦 (Hordeum vulgare L. distichon) 谷物中的烷基间苯二酚”Z.Naturforsch。

Y.Esumi, Y.Suzuki, Y.Itoh, M.Uramoto, K.Kiumra, M.Yoshihama, T.Ichikawa: "Propeptin, a new inhibitor of polyl endopeptidase produced by Microbispora II. Determination of chemical structure"J.Antibiotic.. 55・3. 296-300 (2002)

Y.Esumi、Y.Suzuki、Y.Itoh、M.Uramoto、K.Kiumra、M.Yoshihama、T.Ichikawa：“Propeptin，一种由小双孢菌 II 产生的新型多聚内肽酶抑制剂。结构化学的测定”J.Antibiotic .. 55・3. 296-300 (2002)