Molecular design of lysozyme for switching the antimicrobial action

为了扩大对革兰氏阴性和革兰氏阳性菌的抑菌作用，对蛋清溶菌酶进行了基因修饰的分子设计。将五亲水多肽(Phe-Phe-Val-Ala-Pro)融合到溶菌酶的C末端。得到的贝多肽融合溶菌酶(H5-LZ)对革兰氏阴性和阳性细菌均有较强的杀菌作用。在毕赤酵母表达系统中，H5-LZ的分泌量大大增加，而在酵母菌中的分泌量很少。酿酒。H5-LZ的构象保持折叠状态，但稳定性略有下降，反映了疏水肽的融合。H5-LZ可用于工业应用。作为一个典型的例子，H5-LZ通过农杆菌侵染导入烟草叶片。转基因烟草对灰霉病和白粉病表现出较强的抗性。

In order to expand the antimicrobial action to Gram-negative and Gram-positive, the molecular design of hen egg white lysozyme was attempted by genetic modification. The penta hydrophobic peptide (Phe-Phe-Val-Ala-Pro) was fused to the C terminus of lysozyme. The resulting petapeptide fused lysozyme (H5-Lz) showed strong bactericidal action against Gram-negative and -positive bacteria. The secretion amount of H5-Lz greatly increased in Pichia pastoris expression system, although it was very poor in Saccharomyces . cerevisiae. The conformation of H5-Lz was kept in a folded form, but the stability was lightly decreased, reflecting the fusion of hydrophobic peptide. The H5-Lz can be useful for industrial applications. As a typical example, the H5-Lz was introduce into tobacco leaf through agrobacterium infection. The transgenic tobacco showed strong resistance to gray mold and powdery mildew infection.