Reduction of antigenicity of allergen proteins by the attachment of polysaccjarides and induction of immune tolerance

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An attempt was made to mask the allergenic structure of major allergen protein, Cryj 1., in Japanese cedar pollen using the Maillard-type polysaccharide conjugation. The SDS-PAGE pattern of the Cryj 1-galactomannan conjugate prepared by Maillard reaction showed the broad bands widely distributed from 50 kDa to more than 100 kDa, suggesting the attachment of galactomannan. The competitive enzyme linked immunosorbent assay revealed that the IgE antibody in the sera of ceder pollen-sensitive patients strongly reacted with Cryj 1, while it did not react with the Cryj 1-galactomannan conjugate. This result suggests that the allergenicity of Cryj 1 is greatly reduced by the conjugation with galactomannan. The soy protein-chitosan conjugate was also formed by the Maillard reaction in dry state (relative humidity 65%) at 60 ℃ for 2 weeks to improve the functional properties. The antimicrobial activity of the Maillard-type soy protein-chitosan conjugates enhanced 2〜3 times that of soy protein-chitosan mixture. The soy protein-chitosan conjugate showed excellent emulsifying property with the progress of Maillard-type conjugation. The allergenicity of soy protein was greatly decreased by the attachment of chitosan through Maillard reaction. The immonoblotting analysis with patient's sera revealed that soy protein-chitosan conjugate was more effective to mask the allergen structure of soy protein causing from 34kDa-protein (Gly m Bd 30K) than soy protein-galactomannan conjugate. The western blotting showed that allergen (34kDa-protein) was completely masked by soy protein-chitosan conjugation, while it was not completely masked by soy protein galactomannan conjugation