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Analysis of protective antigen and virulence factors of Lactococcus garvieae, fish bacterial pathogen

鱼类病原菌加维乳球菌的保护性抗原及毒力因子分析

基本信息

批准号：
12660176
负责人：
YOSHIDA Terutoyo
金额：
$ 2.18万
依托单位：
Miyazaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

ABSTRACT : Strains of Lactococcus garvieae were examined for the presence of cell surface structures. Two types of cell capsular strains such as highly developed capsule (KG9408) and micro-capsule (MS93003) of L. garvieae were found, carrying fimbrie-like components, projecting from cell surface. While one stain (NSS9319) has neither cell capsular nor fimbrie-like structures on its cell surface. Highly developed cell capsular strain indicated higher virulence to fish than that of micro-capsular or non-capsular strain. Bacteriphage susceptibilities to these three strains were clearly distinguished from KG9408 to MS93003 and NSS9310 strains. Immune protection against Lactococcus garvieae infection was induced in yellowtail, Seriola guinqueradiata immunized with formalin-killed L. garvieae KG9408 and MS93003. However immune protection was also induced by immunization of NSS9310, the level of protection was significantly lower than that of KG9408 andMS93003. protection was induced by immunization of NSS9310. Passive immunization with yellowtail immune sera raised against KG9408 and MS93003 conferred strong protection to yellowtail with the rapid bacterial clearance after the infection of L. garvieae. Immunoblotting analysis of proteins extracted from L. garvieae strains using rabbit anti-KG9408 and MS93003 serum, and yellowtail anti-KG9408 and MS93003 serum indicated that some bands from NSS9310 were not detected as seen in KG9408 and MS93003 strains.

摘要：检查了伽维乳球菌菌株的细胞表面结构。对L. garvieae，携带菌毛样成分，从细胞表面突出。而一种染色剂（NSS 9319）在其细胞表面上既没有细胞被膜也没有菌毛样结构。高度发育的细胞囊型菌株对鱼类的毒力高于微囊型和无囊型菌株。从KG 9408到MS 93003和NSS 9310菌株，噬菌体对这三种菌株的亲和性明显不同。用福尔马林灭活的格氏乳球菌（Lactococcus garvieae）免疫黄尾鱼（Seriola guinqueradiata），诱导其对格氏乳球菌感染的免疫保护。garvieae KG 9408和MS 93003。但NSS 9310也能诱导免疫保护作用，但保护作用明显低于KG 9408和MS 93003。通过NSS 9310的免疫诱导保护。用抗KG 9408和MS 93003的黄尾鱼免疫血清被动免疫黄尾鱼，在感染L. Garvieae。免疫印迹分析从L.用兔抗-KG 9408和MS 93003血清以及黄尾鼠抗-KG 9408和MS 93003血清对鸡球虫菌株进行的检测表明，在KG 9408和MS 93003菌株中没有检测到来自NSS 9310的一些条带。