Analysis of protective antigen and virulence factors of Lactococcus garvieae, fish bacterial pathogen

鱼类病原菌加维乳球菌的保护性抗原及毒力因子分析

基本信息

批准号：
12660176
负责人：
YOSHIDA Terutoyo
金额：
$ 2.18万
依托单位：
Miyazaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

ABSTRACT : Strains of Lactococcus garvieae were examined for the presence of cell surface structures. Two types of cell capsular strains such as highly developed capsule (KG9408) and micro-capsule (MS93003) of L. garvieae were found, carrying fimbrie-like components, projecting from cell surface. While one stain (NSS9319) has neither cell capsular nor fimbrie-like structures on its cell surface. Highly developed cell capsular strain indicated higher virulence to fish than that of micro-capsular or non-capsular strain. Bacteriphage susceptibilities to these three strains were clearly distinguished from KG9408 to MS93003 and NSS9310 strains. Immune protection against Lactococcus garvieae infection was induced in yellowtail, Seriola guinqueradiata immunized with formalin-killed L. garvieae KG9408 and MS93003. However immune protection was also induced by immunization of NSS9310, the level of protection was significantly lower than that of KG9408 andMS93003. protection was induced by immunization of NSS9310. Passive immunization with yellowtail immune sera raised against KG9408 and MS93003 conferred strong protection to yellowtail with the rapid bacterial clearance after the infection of L. garvieae. Immunoblotting analysis of proteins extracted from L. garvieae strains using rabbit anti-KG9408 and MS93003 serum, and yellowtail anti-KG9408 and MS93003 serum indicated that some bands from NSS9310 were not detected as seen in KG9408 and MS93003 strains.

摘要：检查了Garvieae乳球菌的菌株是否存在细胞表面结构。发现了两种类型的细胞囊菌株，例如高度发达的胶囊（KG9408）和L. garvieae的微胶囊（MS93003），它们携带fimbrie样成分，它们从细胞表面投射出来。虽然一种污渍（NSS9319）在其细胞表面既没有细胞囊囊或类似纤维状的结构。高度发达的细胞囊菌株表明，与微样或非囊肿菌株相比，对鱼类的毒力更高。对这三种菌株的细菌敏感性与KG9408至MS93003和NSS9310菌株明显区分。在Yellowtail，Seriola Guinqueradiata诱导了对GARVIEAE感染的免疫保护，并用福尔马林杀性的L. garvieae KG9408和MS93003免疫。但是，NSS9310的免疫也诱导了免疫保护，保护水平明显低于KG9408和MSS93003。 NSS9310的免疫诱导了保护。用黄尾尾免疫血清对KG9408和MS93003饲养的被动免疫，并在感染Garvieae后的快速细菌清除率赋予了强烈的保护。使用兔抗KG9408和MS93003血清中提取的蛋白质的免疫印迹分析，以及Yellowtail Anti-KG9408和MS93003血清的免疫印迹分析，表明在KG9408和MS93003 STRANS中未发现NSS9310的某些乐队。