Epidemiological studies and diagonosis of Streptococcus dysgalactiae isolated farmed fish

养殖鱼类停乳链球菌流行病学研究及诊断

基本信息

批准号：
17580165
负责人：
YOSHIDA Terutoyo
金额：
$ 2.3万
依托单位：
University of Miyazaki
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

The Lancefield serological group C Streptococcus sp. was isolated from cultured amberjack, Seriola dumerili Risso and yellowtail, Seriola quinqueradiata Temminck and Schlegel that had been immunized with Lactococcus garvieae commercial vaccines in Japan. The isolated bacteria were gram-positive cocci, auto-aggregated in saline, morphologically long chains in growth medium, catalase negative and α-haemolysis on blood agar. An almost complete gene sequence of the 16S rDNA of isolates (n=2) was determined and compared to that of bacterial strains in the data base. The isolates were identified to Streptococcus dysgalactiae based on the results of the 16S rDNA sequence, the bacteriological properties and the Lancefield serological grouping. The oligonucleotide primers designed with specifity for the 16S-23S rDNA intergenic spacer region of S. dysgalactiae amplified a gene from all the fish isolates as well as the type strains of α-haemolytic S. dysgalactiae subsp. dysgalactiae ATCC430738 and β-haemolytic S. dysgalactiae subsp. equisimilis ATCC35666, but not those of S. equiATCC33398, Lactococcus garvieaeATCC43921 and L. garvieae KG94 08. The severe lesion (a necrotic lesion of the caudal peduncle) observed on experimentally infected fish was similar to that seen on naturally infected fish. Further attention is required to distinguish the Lancefield group C Streptococcal infection from L. garvieae infection in S. dumerili and S. quinqueradiata in Japan.

兰斯菲尔德血清学组C链球菌Sp。从日本的培养的Amberjack，Seriola Dumerili Risso和Yellowtail，Seriola Quinqueradiata temminck和Schlegel分离出来，这些seriola Quinqueradiata temminck和Schlegel在日本已用Garviae lactocococcus Garviae商业疫苗进行了免疫。分离的细菌是革兰氏阳性球菌，在盐水中自动聚集，在生长培养基中的形态上长链，过氧化氢酶阴性和血液琼脂的α-释放分解。确定了分离株（n = 2）的16S rDNA的几乎完整基因序列，并将其与数据库中细菌菌株的基因序列进行比较。根据16S rDNA序列，细菌特性和兰斯菲尔德血清学分组的结果，将分离株鉴定为肌肉链球菌的链球菌。寡核苷酸引物设计为特异性针对链球菌的16S-23S rDNA rDNA间间隔区域扩增了所有鱼类08的基因。严重的病变（在实验感染的鱼类上观察到的严重病变（尾部的caudale peduntunce caudale pedunture）与自然感染的鱼类相似。需要进一步的注意来区分日本的杜梅利（S. dumerili）和昆quinqueradiata中的兰斯菲尔德（Lancefield）链球菌感染与Garvieae感染的链球菌感染。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Application of attenuated Lactococcus garvieae strain lacking a virulence associated capsule on its cell surface as a live vaccine in yellowtail Seriola quinqueradiata Temminck & Schlegel

细胞表面无毒力相关荚膜的加氏乳球菌减毒株作为黄尾鰤活疫苗的应用

DOI：
发表时间：
2006
期刊：
Journal of applied Ichthyology 22(2)(In press)
影响因子：
0
作者：
Tsuyoshi Ooyama^1;Yoshiko Shimahara^2;Ryohei Nomoto^2;Hiroshi Yasuda^1;Kazuo Iwata^1;Atsushi Nakamura^1;Toshiaki Itami^2;Terutoyo Yoshida^<2*>
通讯作者：
Terutoyo Yoshida^<2*>

Detection of antibody response against Nocardia seriolae by enzyme-linked immunosorbent assay(ELISA) and a preliminary vaccine trial in yellowtail Seriola quinqueradiata

酶联免疫吸附试验（ELISA）检测鰤诺卡氏菌抗体反应及黄尾鰤疫苗初步试验