Epidemiological studies and diagonosis of Streptococcus dysgalactiae isolated farmed fish

养殖鱼类停乳链球菌流行病学研究及诊断

• 批准号: 17580165
• 负责人: YOSHIDA Terutoyo
• 金额: $ 2.3万
• 依托单位: University of Miyazaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17580165/
• 关键词: Lancefield group C; Streptococcus dysgalactiae; Seriola quinqueradiata; Seriola dumerili; Characterization; 新型連鎖球菌; カンパチ; ブリ; 養殖魚類; 疫学; 早期診断

The Lancefield serological group C Streptococcus sp. was isolated from cultured amberjack, Seriola dumerili Risso and yellowtail, Seriola quinqueradiata Temminck and Schlegel that had been immunized with Lactococcus garvieae commercial vaccines in Japan. The isolated bacteria were gram-positive cocci, auto-aggregated in saline, morphologically long chains in growth medium, catalase negative and α-haemolysis on blood agar. An almost complete gene sequence of the 16S rDNA of isolates (n=2) was determined and compared to that of bacterial strains in the data base. The isolates were identified to Streptococcus dysgalactiae based on the results of the 16S rDNA sequence, the bacteriological properties and the Lancefield serological grouping. The oligonucleotide primers designed with specifity for the 16S-23S rDNA intergenic spacer region of S. dysgalactiae amplified a gene from all the fish isolates as well as the type strains of α-haemolytic S. dysgalactiae subsp. dysgalactiae ATCC430738 and β-haemolytic S. dysgalactiae subsp. equisimilis ATCC35666, but not those of S. equiATCC33398, Lactococcus garvieaeATCC43921 and L. garvieae KG94 08. The severe lesion (a necrotic lesion of the caudal peduncle) observed on experimentally infected fish was similar to that seen on naturally infected fish. Further attention is required to distinguish the Lancefield group C Streptococcal infection from L. garvieae infection in S. dumerili and S. quinqueradiata in Japan.

兰斯菲尔德血清学C群链球菌是从日本用加氏乳球菌商业疫苗免疫的培养的琥珀鱼、杜氏seriola dumerili Risso和黄尾Seriola quinqueradiata Temminck和Schlegel中分离的。分离到的细菌为革兰氏阳性球菌，在生理盐水中自聚集，在生长培养基中形态为长链，过氧化氢酶阴性，在血琼脂上呈α-溶血性。一个几乎完整的基因序列的16 S rDNA的分离物（n=2）进行了测定和比较的数据库中的细菌菌株。根据16 S rDNA序列测定、细菌学特性和兰斯菲尔德血清学分型结果，将分离菌株鉴定为停乳链球菌。设计了一对特异性针对16 S-23 SrDNA基因间隔区的寡核苷酸引物。dysgalactiae从所有鱼类分离株以及α-溶血性S.停乳亚种停乳链球菌ATCC 430738和β-溶血性链球菌。停乳亚种equisimilis ATCC 35666，而S. equiATCC 33398、Lactococcus garvieae ATCC 43921和L. Garvieae KG 94 08.在实验感染的鱼上观察到的严重病变（尾柄坏死病变）与在自然感染的鱼上观察到的病变相似。需要进一步注意区分兰斯菲尔德C组链球菌感染和L.感染S. dumerili和S.产于日本。

项目成果

Application of attenuated Lactococcus garvieae strain lacking a virulence associated capsule on its cell surface as a live vaccine in yellowtail Seriola quinqueradiata Temminck & Schlegel

细胞表面无毒力相关荚膜的加氏乳球菌减毒株作为黄尾鰤活疫苗的应用

• 发表时间: 2006
• 期刊: Journal of applied Ichthyology 22(2)(In press)
• 作者: Tsuyoshi Ooyama^1;Yoshiko Shimahara^2;Ryohei Nomoto^2;Hiroshi Yasuda^1;Kazuo Iwata^1;Atsushi Nakamura^1;Toshiaki Itami^2;Terutoyo Yoshida^<2*>
• 通讯作者: Terutoyo Yoshida^<2*>

Detection of antibody response against Nocardia seriolae by enzyme-linked immunosorbent assay(ELISA) and a preliminary vaccine trial in yellowtail Seriola quinqueradiata

酶联免疫吸附试验（ELISA）检测鰤诺卡氏菌抗体反应及黄尾鰤疫苗初步试验

• 发表时间: 2005
• 期刊: Bulletin of the European Association of Fish Pathologists 25(6)
• 作者: Y.Shimahara;H.Yasuda;A.Nakamura;T.Itami;T.Yoshida
• 通讯作者: T.Yoshida

新魚病図鑑 レンサ球菌症(ストレプトコカス・ジスガラクチエ感染症)

新鱼病百科全书链球菌病（停乳链球菌感染）

• 发表时间: 2006
• 作者: 野本竜平;吉田照豊
• 通讯作者: 吉田照豊

新魚病図鑑(レンサ球菌症 ストレプトコッカス ジスガラクチエ感染症)

新鱼病百科全书（脱乳链球菌感染）

• 发表时间: 2005
• 作者: 野本竜平;吉田照豊
• 通讯作者: 吉田照豊

Characterization of Lancefield group C Streptococcus dysgalactiae isolated from farmed fish

• DOI: 10.1111/j.1365-2761.2006.00763.x
• 发表时间: 2006-11-01
• 期刊: JOURNAL OF FISH DISEASES
• 影响因子: 2.5
• 作者: Nomoto, R.;Unose, N.;Yoshida, T.
• 通讯作者: Yoshida, T.