Risk assessments of the emerging fish pathogen in the environmental condition of rising water temperature.

水温上升环境条件下新出现的鱼类病原体的风险评估。

• 批准号: 21580229
• 负责人: YOSHIDA Terutoyo
• 金额: $ 3万
• 依托单位: University of Miyazaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580229/
• 关键词: C群レンサ球菌; Streptococcus dysgalactiae; 高水温; ブリ; カンパチ; 魚病; 抗原; 薬剤感受性; レンサ球菌; ブリ属魚類; streptococcus dysgalactiae; ランスフィールドC群; 抗原型; コンゴーレッド

TH-CR(Todd-Hewitt Congo red) agar was developed to detect and presumptively identify the GCSD(Group C Streptococcus dysgalactiae) obtained from artificially or naturally infected fish. Typical symptoms were observed in artificially infected fish at higher temperature(28°C), thus need an attention at the high water temperature season. The genetic analysis revealed that the fish isolates were genetically very similar to each other with high DNA. DNA relatedness and sequence homology. Seventeen isolates in GCSD collected Asian countries were found to be resistant to oxytetracycline and carried the tet(M) gene, except for the strains collected in Taiwan and the PP1564 strain collected in China. The amino acid sequence of SOF was identical among fish isolates. A primer set targeting the sof-FD gene was designed and applied to a PCR assay for discriminating fish isolates from mammalian isolates.

研制了一种检测和推定C组停乳链球菌（Streptococcus dysgalactiae，GCSD）的TH-CR（Todd-Hewitt刚果红）琼脂。人工感染的鱼在较高温度（28°C）下观察到典型症状，因此在高水温季节需要注意。遗传分析表明，鱼类分离物在遗传上彼此非常相似，具有高DNA。DNA相关性和序列同源性。在GCSD收集的亚洲国家中，发现17株分离株对土霉素具有耐药性，并携带泰特（M）基因，但台湾收集的菌株和中国收集的PP 1564菌株除外。SOF的氨基酸序列在鱼类分离物中是相同的。针对sof-FD基因设计了一对引物，并将其应用于PCR检测，用于区分鱼类分离株和哺乳动物分离株。

项目成果

Homogeneity of Streptococcus dysgalactiae from farmed amberjack Seriola dumerili in Japan

• DOI: 10.1007/s12562-010-0243-5
• 发表时间: 2010-07-01
• 期刊: FISHERIES SCIENCE
• 影响因子: 1.9
• 作者: Nishiki, Issei;Noda, Masahiro;Yoshida, Terutoyo
• 通讯作者: Yoshida, Terutoyo

Dissemination of streptococcal pyrogenetic exotoxin G(spegg) with an IS-like element in fish isolates of Streptococcus dysgalactiae

具有 IS 样元素的链球菌热生外毒素 G（spegg）在停乳链球菌鱼分离株中的传播

• 发表时间: 2010
• 期刊: FEMS Microbiology Letters
• 影响因子: 2.1
• 作者: M. Abdelsalam;S. C. Chen and T. Yoshida
• 通讯作者: S. C. Chen and T. Yoshida

Homogeneity of Streptococcus dysgalactiae isolated from amberjack, Seriola dumerili, in Japan

日本从鰤鰤分离的停乳链球菌的同质性

• 发表时间: 2010
• 期刊: Fisheries Science
• 影响因子: 1.9
• 作者: I. Nishiki;M. Noda;T. Itami and T. Yoshida
• 通讯作者: T. Itami and T. Yoshida

Cloning and expression of serum opacity factor in fish pathogenic Streptococcus dysgalactiae and its application to discriminate between fish and mammalian isolates

鱼类病原性停乳链球菌血清混浊因子的克隆和表达及其在鱼类和哺乳动物分离中的应用

• 发表时间: 2011
• 期刊: FEMS Microbiology Letter
• 作者: Nishiki;Y. Horikiri;T. Itami and T. Yoshida
• 通讯作者: T. Itami and T. Yoshida

Phenotypic and genetic characterization of Streptococcus dysgalactiae strains isolated from fish collected in Japan and other Asian countries

从日本和其他亚洲国家收集的鱼类中分离出的停乳链球菌菌株的表型和遗传特征

• 发表时间: 2010
• 期刊: FEMS Microbiology Letters
• 影响因子: 2.1
• 作者: M.Abdelsalam;S.C.Chen;T.Yoshida
• 通讯作者: T.Yoshida