Selective Removal of Environmental Hormones by Biopolymer Conjugates

研究了DNA、环糊精等生物相关聚合物偶联物对环境激素的选择性去除作用。含环糊精的材料选择性地与双酚A和壬基酚结合。含有dna的物质与二恶英衍生物和苯并芘结合，选择性和非常有效。因此这个项目主要集中在DNA材料上。DNA具有独特的化学性质，很容易从鲑鱼的手套和贝类的性腺中纯化出来。DNA几乎没有商业用途，通常作为工业废物丢弃。采用紫外照射法、透析法、溶胶-凝胶法和合成聚合物偶联法制备了不溶于水的固定化含dna材料。dna固定珠柱结合具有平面结构的内分泌干扰物，如二恶英衍生物、多氯联苯衍生物、苯并芘。双酚A和己烯雌酚缺乏平面结构，不能与dna固定柱结合。这些结果表明，DNA固定珠柱可以选择性地将具有平面结构的化合物插入到双链DNA中。dna固定化微珠有潜力作为有用的环境应用生物材料，如香烟过滤器和空气净化器。

Selective removal of environmental hormones by some biorelated polymer conjugates, such as DNA and cyclodextrin-containing polymeric material was investigated. Cyclodextrin-containing materials bind selectively to bisphenol A and nonylphenol. DNA-containing materials bind to dioxin derivatives and benzopyrens, selectively and very effectively. Hence this project focused mainly on DNA materials.DNA has unique chemical properties and is readily purified from salmon mitts and shellfish gonads. DNA has few commercial uses and is generally discarded as an industrial waste. We prepared water-insoluble and immobilized DNA-containing materials by UV-irradiation, dialysis method, sol-gel method and conjugation with synthetic polymer. The DNA-immobilized beads columns bound endocrine disruptors with a planar structure, such as dioxin-derivatives, polychlorobiphenyl (PCB)-derivatives, benzopyrene. Bisphenol A and diethylstilbestrol, which lack a planar structure, did not bind to the DNA-immobilized columns. These results suggest that DNA-immobilized bead columns could selectively remove chemical compounds with a planar structure by intercalation into the double-stranded DNA. DNA-immobilized beads have the potential to serve as useful biomaterials for environmental applications, such as filter of cigarette and air cleaner.