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Selective Removal of Environmental Hormones by Biopolymer Conjugates

生物聚合物缀合物选择性去除环境激素

基本信息

批准号：
14350491
负责人：
NISHI Norio
金额：
$ 8.26万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14350491/
关键词：
biopolvmer conjugate environmental hormone dialysis dioxin benzopyrene cigarette filter air cleaner sol-gel method DNA 選択的除去シクロデキストリン PCB

项目摘要

Selective removal of environmental hormones by some biorelated polymer conjugates, such as DNA and cyclodextrin-containing polymeric material was investigated. Cyclodextrin-containing materials bind selectively to bisphenol A and nonylphenol. DNA-containing materials bind to dioxin derivatives and benzopyrens, selectively and very effectively. Hence this project focused mainly on DNA materials.DNA has unique chemical properties and is readily purified from salmon mitts and shellfish gonads. DNA has few commercial uses and is generally discarded as an industrial waste. We prepared water-insoluble and immobilized DNA-containing materials by UV-irradiation, dialysis method, sol-gel method and conjugation with synthetic polymer. The DNA-immobilized beads columns bound endocrine disruptors with a planar structure, such as dioxin-derivatives, polychlorobiphenyl (PCB)-derivatives, benzopyrene. Bisphenol A and diethylstilbestrol, which lack a planar structure, did not bind to the DNA-immobilized columns. These results suggest that DNA-immobilized bead columns could selectively remove chemical compounds with a planar structure by intercalation into the double-stranded DNA. DNA-immobilized beads have the potential to serve as useful biomaterials for environmental applications, such as filter of cigarette and air cleaner.

研究了一些生物相关聚合物缀合物（例如 DNA 和含环糊精的聚合物材料）选择性去除环境激素的情况。含环糊精的材料选择性地与双酚A和壬基酚结合。含有 DNA 的材料可以选择性且非常有效地与二恶英衍生物和苯并芘结合。因此，该项目主要关注 DNA 材料。DNA 具有独特的化学性质，很容易从鲑鱼手套和贝类性腺中纯化。 DNA几乎没有商业用途，通常作为工业废物丢弃。我们通过紫外线照射、透析法、溶胶-凝胶法以及与合成聚合物缀合制备了不溶于水的固定化含DNA材料。 DNA 固定珠柱以平面结构结合内分泌干扰物，例如二恶英衍生物、多氯联苯 (PCB) 衍生物、苯并芘。双酚 A 和己烯雌酚缺乏平面结构，不会与 DNA 固定柱结合。这些结果表明，DNA 固定珠柱可以通过插入双链 DNA 来选择性去除具有平面结构的化合物。 DNA 固定珠有潜力成为环境应用中有用的生物材料，例如香烟过滤嘴和空气净化器。