Selective Removal of Environmental Hormones by Biopolymer Conjugates

研究了生物相关高分子复合物（如DNA和环糊精）对环境激素的选择性去除。含环糊精的材料选择性地与双酚A和壬基酚结合。含有DNA的物质与二恶英衍生物和苯并芘结合，选择性和非常有效。因此，本项目主要集中在DNA材料上，DNA具有独特的化学性质，易于从鲑鱼的手套和贝类生殖腺中纯化。DNA几乎没有商业用途，通常作为工业废物被丢弃。我们采用紫外光照射、透析法、溶胶-凝胶法和人工合成高分子偶联法制备了水不溶性DNA固定化材料。DNA固定化微球柱以平面结构结合二恶英衍生物、多氯联苯（PCB）衍生物、苯并芘等内分泌干扰物。双酚A和己烯雌酚，缺乏平面结构，没有绑定到DNA固定柱。这些结果表明，DNA固定化珠柱可以通过嵌入双链DNA选择性地去除具有平面结构的化合物。DNA固定化微球具有作为环境生物材料的潜力，如香烟过滤嘴、空气净化器等。

Selective removal of environmental hormones by some biorelated polymer conjugates, such as DNA and cyclodextrin-containing polymeric material was investigated. Cyclodextrin-containing materials bind selectively to bisphenol A and nonylphenol. DNA-containing materials bind to dioxin derivatives and benzopyrens, selectively and very effectively. Hence this project focused mainly on DNA materials.DNA has unique chemical properties and is readily purified from salmon mitts and shellfish gonads. DNA has few commercial uses and is generally discarded as an industrial waste. We prepared water-insoluble and immobilized DNA-containing materials by UV-irradiation, dialysis method, sol-gel method and conjugation with synthetic polymer. The DNA-immobilized beads columns bound endocrine disruptors with a planar structure, such as dioxin-derivatives, polychlorobiphenyl (PCB)-derivatives, benzopyrene. Bisphenol A and diethylstilbestrol, which lack a planar structure, did not bind to the DNA-immobilized columns. These results suggest that DNA-immobilized bead columns could selectively remove chemical compounds with a planar structure by intercalation into the double-stranded DNA. DNA-immobilized beads have the potential to serve as useful biomaterials for environmental applications, such as filter of cigarette and air cleaner.