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Three-dimensional analysis of the morphogenesis of nerve branches and vessels distributed to the heart and bronchial organs of embryos, using whole-mount immunohistochemical staining and moleculohistoochemical methods.

使用整体免疫组织化学染色和分子组织化学方法对分布到胚胎心脏和支气管器官的神经分支和血管的形态发生进行三维分析。

基本信息

批准号：
12670010
负责人：
TANAKA Shigenori
金额：
$ 2.18万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670010/
关键词：
development peripheral nerve heart humans house shrew chick rat immunolhistochemistry

项目摘要

In year of 2000, the branches of the synpathetic nerve which reach the heart through the venous porta was newly discovered in the hearts of house shrew ( Smcus miriws). These nerve branches were observed to originate from the sympathetic trunk, and to course the azygos vein which course in parallel with this sympathetic trunk. Thereafter, these sympathetic (cardiac) nerve branches take a course along the brachiocephalic and superior caval vein, - entering the venous porta of the heart. These nerve branches are not observable through the common anatomical methods, and have not described in scientific papers. In our study, the existence of these heart nerve branch of this sympathetic nerve were clarified-by the method of what is called "whole-mount immunohistochemical staining method".In 2001, on the base of the data mentioned above, we researched concerning the existence of the cardiac nerve of the sympathetic nerve trunk origin in human beings. The observation area of humans is greatly … More wide. The cardiac nerve branches of the sympathetic nerve trunk origin are thinner than the cardiac branches of the vagus nerve origin, and their form is more complex than that of the latter. Therefore, it is difficult in a usual and macroscopic anatomy methods to investigate the concerned branches completely, and to name them. In two (2001 and 2002) years of our research details of the cardiac nerve which arise from the cervix part and the chest sympathetic nerve trunk are sketched in the A2 papers. We have clarified the cardiac nerve branches with the embryology form concept of the arterial and venous portae of the heart.It turned out all nerves distributed in human heart can be named according.to the same terms which we have applied to the cardiac nerve branches of the house shrews (Tanaka et al, 1988).From now on, in our laboratory, embryological researches on the heart nerve branch are scheduled to be proceeded furthermore, while giving a deep mind to the data of humans.Details of the formation of raf s knee joint were also elucidated in the study on the nerve developmental formation of the knee joint. By immunohistochemical staining and biochemical research, it was clarified that the knee joint is not formed with the apoptosis of the cell as hitherto described, but with the ordinal cell development. Less

2000年，在家？心脏中发现了经静脉门到达心脏的交感神经分支。观察到这些神经分支起源于交感干，并走行奇静脉，奇静脉与交感干平行走行。此后，这些交感（心脏）神经分支沿着头臂静脉和上级腔静脉行进，进入心脏的静脉门。这些神经分支不能通过普通解剖学方法观察到，也没有在科学论文中描述。本研究采用“整体包埋免疫组织化学染色法”，明确了交感神经干起源的心神经分支的存在，2001年在此基础上，对交感神经干起源的心神经的存在进行了研究。人类的观察范围 ...更多信息宽交感神经干起源的心神经支较迷走神经起源的心神经支细，形态较迷走神经起源的心神经支复杂。因此，用一般的宏观解剖方法很难对有关分支进行全面的研究，并对其命名。我们在2001年和2002年两年的研究中，在A2论文中描绘了起源于颈部的心神经和胸交感神经干的详细情况。本文用心脏动、静脉门的胚胎学形态概念阐明了心脏的神经分支，结果表明，分布于人心脏的所有神经都可以according.to用家鼠心脏神经分支的名称来命名（Tanaka等，1988）。从现在起，在我们的实验室中，计划进一步进行心脏神经分支的胚胎学研究，同时对人类的数据进行了深入的研究，并在膝关节神经发育形成的研究中阐明了raf膝关节形成的细节。通过免疫组织化学染色和生物化学研究，阐明了膝关节不是由迄今为止所描述的细胞凋亡形成的，而是由有序细胞发育形成的。少