Elucidation of three dimension structure of the cardiac nerves and branches and of liver- distributing arteries in the adults and embryos of human and muskrat beavers, using our unique technology and whole mount in situ hybridication

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We have elucidated three-dimensional structure of the cardiac nerves which originate from sympathetic nerves and distribute to the heart, coursing cardiac arterial and venous gates, Furthermore, we have endeavored to clarify blood vessels coursing in the floor of urinary bladder ; umbilical fold, and in the farciform et round ligaments of liver.Clarification of vagus nerve branches, including cardiac branches, is not difficult, because these branches course only on or in the internal organs. Thereagainst, these sympathetic nerves originate from the sympathetic nerves never to the vertebral columun. Therefore, the cardiac nerves are very numerousness and complex form.Therefore, three-dimensional clarification of these cardiac nerves is very difficult. Especially, the anatomical area in human bodies is exceedingly large, including adipose. We have developed technology in order to stain nerves and vessels. Using this technology, we have clarified the structure of human cardiac nerves.Acco … More rding to these results, we have coached a doctoral student.This doctoral student has elucidated three-dimensional structure of nerves and arteries distributing to the heart in the musk shrew This research fining was inserted in Anat.Rec. (Hoboken 290,2007) Achievement of nerves supplying cholecyst of human and musk shrew was inserted in Aorld J. Gastroenteral (13, 2007)Also, we make histological clarification, using electron microscope, and link up with academic researchers of the Molecular and Genetic Science. The result was inserted in Nature (Vol.446, 2007)We also coached master's student in order to clarigy arteries coursing in apex of the bladder, umbilicus, falciform ligament of liver ; round ligament of liver.This fruit of research was determined as graduation thesis in 2007.In all human donor bodies, using above noted research technology, we have first clarified arteries coursing from the abdominant wall to the liver, and existence of the allantoic stalk. We are providing to complete thesis. Less