课题基金 / 基金详情

Deep observational researches regarding the profound structures, morphogenesis and regeneration of nerves, bloods and muscles existing in the visceral internal organs as well as four limbs, using sui genesis technology of whole-mount staining, and molecul

Deep observational researches regarding the profound structures, morphogenesis and regeneration of nerves, bloods and muscles existing in the visceral internal organs as well as four limbs, using sui genesis technology of whole-mount staining, and molecul
利用整体染色、分子生物学等独特成因技术,对内脏、四肢等神经、血液、肌肉的深层结构、形态发生和再生进行深入观察研究
批准号:
16590139
负责人:
TANAKA Shigenori
金额:
$2.24万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2004
资助国家:
日本
项目状态:
已结题
起止时间:
2004 至 2005

项目摘要

项目成果

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中文摘要
翻译
进一步推进了对心脏内分布的交感神经即心神经形态结构的研究。我们发现心神经从交感神经干的广大区域大量产生,它们之间的联系非常广泛。此外,它们的跑步模式或形式比迷走神经的心脏分支要复杂得多。因此,这些心脏神经的特征比迷走神经的心脏分支更难以明确。其中,适用于研究的人类样本比其他脊椎动物的样本要大得多,而且它们之间相互交流,它们的运动图形比迷走神经的心脏分支要复杂得多。因此,要完全厘清人类心脏神经是一项困难的课题。为了解决这个困难,我们采用了新的专用技术。利用这项技术,我们能够…用酒精涂抹更多的酒精,用arizarin red S染色神经,并在所有人类和其他脊椎动物的实验样本中阐明血管。因此,心脏神经被发现起源于交感神经干,从颈部到最低胸椎水平,即ThI0(在人的例子中还有ThI2)的广泛区域。此外,不仅从人体解剖学的角度,而且从脊椎动物比较解剖学和胚胎学的角度,对人体尸体的三维结构进行了基本的澄清。我们已经在学术会议上发表了这些结果(日本临床解剖学研究会8^< >会议和日本外周神经研究会16^< >会议)。因此,正在对国际科学杂志关于心脏神经和分支形式的投稿稿进行润色和更正。采用鼠类比小鼠更接近人的鼠类全胚胎免疫染色方法对心脏神经的形态和形态发生进行了澄清(博士课程学生提交论文)。该研究结果发表在日本解剖学学会第110期。国际科学杂志的投稿也正在准备中。麝鼠肝脏生成的形式,临床医学意义(Endoclinology 2004: Anat.Enbryol.)。2004年)已得到澄清。此外,我们还分析了支配提上腭肌和下颌神经的神经图(clinin Anat 2004; Surg Radiol Anat 2004)。目前,我们正在努力推进研究,以分析肝镰状韧带和肝圆韧带内的动脉、静脉和神经的内部结构和形态发生,阐明剑突周围淋巴管与人体和鼠类的形态和功能关系。这些结构和这些结构的根意义正在从胚胎学和临床的角度进行澄清,建立与临床研究人员的合作。我们还与医生进行合作研究,以开辟新的测量、诊断和治疗骨骼异常的方法,以及头颈部肌肉发生的分子和组织化学研究。少
英文摘要
Researches of elucidation of form and structure of the sympathetic nerve distributed in the heart, that is, cardiac nerves were furthermore promoted.The cardiac nerves were found to arise numerous in number from very broad areas of the sympathetic trunk, communicating each other very numerously.Furthermore, their patterns or forms of running were much more complicated than those of the cardiac branches of the vagus nerve.As a result, clarification of features of these cardiac nerves are difficult than the cardiac branches of the vagus nerve.Inter alia, human examples applicable for researches are exceedingly large as those of other vertebrate and further more, they communicate each other and their coursing figures are much more complicated than those of the cardiac branches of the vagus nerve. Therefore, the complete clarification of human cardiac nerves are difficult article.In order to resolve this difficulty, we applied new specific technology. Using this technology, we are able to … More grease alcohol by using alcohol, stain nerves with arizarin red S, and to elucidate blood vessels in all experimental examples of humans and other vertebrates.In consequence, the cardiac nerves were found to arise from the sympathetic trunk in a wide area from the cervical until the lowest thoracic level, i.e., ThI0 (and further more ThI2) in human examples.Furthermore, the three-dimensional structures of human cadavers have been clarified fundamentally not only from the human anatomical point of view, but also comprehensively from the vertebrate comparative anatomy and embryology.We have been made presentations of these results in academic conferences (the 8^<th> Meeting of Japanese Research Society of Clinical Anatomy and the 16^<th> Meeting of Japanese Research Society of Periphera Nerve).Therewithal, the contribution manuscripts of the international science magazine concerning the form of the cardiac nerves and branches are being to retouched and corrected.The clarification of morphology and morphogenesis of the cardiac nerves are performed by using methods for whole embryo immunity staining in muskrats which are nearer human's form than the mouse and the rat for the form formation (doctor's course student's submitting thesis).These results of this research were presented in the 110th in Japanese Anatomy Association. Contribution to the international science magazine is also being prepared. The form of generation of the muskrat liver, clinical medicine meaning (Endoclinology 2004 : Anat.Enbryol. 2004) has been clarified. Furthermore, figures of the nerves innervating the levator palate muscle and mandibular nerve were elucidated (Clin Anat 2004 ; Surg Radiol Anat 2004).At the moment, we make exertions to advancing researches in order to analyze the intimate structures as well as morphogenesis of arteries, veins and nerves coursing in the falciform and teres hepatic ligaments and to clarify the morphological as well as functional relationships-of lymphatic vessel around the xiphoid process with the live in the human cadavers and also in muskrats. These structures and root meanings of these structures are being clarified from the embryological and clinical viewpoints, building up co-operation with clinical researchers. We are also making collaborative researches with doctors in order to open up new method of measurement, diagnosis and treatment of abnormal bones, and also the molecular and histochemical researches of genesis of head and neck muscles. Less
期刊论文(40)
专著(0)
科研奖励(0)
会议论文
DOI: 10.1359/jbmr.040126
发表时间: 2004-06-01
期刊: JOURNAL OF BONE AND MINERAL RESEARCH
影响因子: 6.2
作者: [Komatsubara, S, Mori, S, Norimatsu, H]
通讯作者: Norimatsu, H
Surgical anatomy of the innervation of the major duodenal papilla in human and Suncus murinus, from the perspective of preserving innervation
从保留神经支配的角度对人和鼠十二指肠乳头的神经支配进行外科解剖
DOI: --
发表时间: 2005
期刊: Pancreas 30・3
影响因子: --
作者: [Yi SQ, Ohta T, Miwa K, Shimokawa T, Akita K, Itoh M, Miyamoto K]
通讯作者: Miyamoto K
Gradual nerve elongation affects nerve cell bodies and neuro-muscular junctions
神经逐渐伸长影响神经细胞体和神经肌肉接头
DOI: --
发表时间: 2005
期刊: Hand and Surgery 10・2
影响因子: --
作者: [Ikeda K, Matuda M, Yamauchi D, Tomita K, Tanaka S]
通讯作者: Tanaka S
DOI: --
发表时间: 2004
期刊: ene comparative endocrinology 136・2
影响因子: --
作者: [Yi SQ, Akita K, Shimokawa T, Nakatani T, Tanaka S et al.]
通讯作者: Tanaka S et al.
共 13 条
    Development for Advanced and Practical Application of Spatial Measurement Technology Using Laser Scanner Equipped UAV
    • 批准号:
      18H01563
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $11.56万
    • 财政年份:
      2018
    • 负责人:
      TANAKA Shigenori
    • 依托单位:
    Development of theoretical methods for interaction analysis of protein-ligand systems in computational drug design
    • 批准号:
      26460035
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $3.16万
    • 财政年份:
      2014
    • 负责人:
      TANAKA Shigenori
    • 依托单位:
    First-principles simulations of water on the basis of quantum Monte Carlo and fragment molecular orbital methods
    • 批准号:
      23540451
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $2.83万
    • 财政年份:
      2011
    • 负责人:
      TANAKA Shigenori
    • 依托单位:
    Elucidation of three dimension structure of the cardiac nerves and branches and of liver- distributing arteries in the adults and embryos of human and muskrat beavers, using our unique technology and whole mount in situ hybridication
    • 批准号:
      18590164
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $2.48万
    • 财政年份:
      2006
    • 负责人:
      TANAKA Shigenori
    • 依托单位:
    海外基金