Molecular and functional morphological studies of M cells

M细胞的分子和功能形态学研究

基本信息

  • 批准号:
    12670532
  • 负责人:
  • 金额:
    $ 1.15万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2001
  • 项目状态:
    已结题

项目摘要

We have studied that M cells can take up a broad range of macromolecules, microorganisms, and particles and transport them to immunocompetent cells in underlying mucosal lymphoid tissues. It has been shown that the M cells of gut associated lymphoid tissue (GALT) have the role of first information conduit" in the initiation of this local intestinal immunity. "The role of the nasopharyngeal tonsils in antigen uptake for initiation of the mucosal immune response is unknown. This project showed that the M cells ofnasopharyngeal lymphoid tissue (NALT) were ultrastructurally and functionally similar to those in human Peyer's patches and colonic lymphoid follicles. These findings indicate that NALT bears similalities to the GALT, and its antigen uptake capacity may be important for initiation of immunity in the upper aerodigestive tract.At present, the effects of these immunosuppressants on Peyer's patch structure and whether they facilitate or retard M cell uptake and treansport of antigens … More are unknown. The present project examined the effects of these immunosuppressants on Peycr's patches, which play an important role in mucosal immune response through uptake and transport of enteric microorganisms and macromolecules in the gut-associated lymphoid tissues. It was shown that FK and Cs may produce immunosuppressive effects, at least in part, through reduction of the uptake and transport of particles into Peyer's patches, and by reduction of the number of immunoreactive cells in FAE of Peyer's patches.Vimentin has previously been shown to be expressed in rabbit M cells of Peyer's patches and appendixes specifically but not in other gastrointestinal epithelial cells. However, we found that immunoelectron microscopy revealed that the labeled cells in villus epithelium have shorter microvilli than do adjacent absorptive cells and small sparese mitochondria, which are ultrastructural characteristics of cup cells. Vimentin filaments were recognized in the area surrounding the nucleus and extending beneath the plasma membrane of cup cells. In rabbit small intestine, vimentin is useful for detection of cup cells as well as M cells and may facilitate investigation of these little understood cells. Less
我们已经研究了M细胞可以摄取广泛的大分子、微生物和颗粒,并将它们转运到粘膜淋巴组织中的免疫活性细胞。肠道相关淋巴组织(GALT)中的M细胞在肠道局部免疫的启动中起着“第一信息管道”的作用。“鼻咽扁桃体在抗原摄取中启动粘膜免疫反应的作用尚不清楚。本研究表明,鼻咽淋巴组织(NALT)中的M细胞在超微结构和功能上与人派伊尔集合淋巴结和结肠淋巴滤泡中的M细胞相似。这些结果表明NALT与GALT具有相似性,其抗原摄取能力可能是上呼吸消化道免疫启动的重要因素,目前,这些免疫抑制剂对Peyer结结构的影响以及它们是否促进或阻碍M细胞对抗原的摄取和转运, ...更多信息 是未知的。本项目研究了这些免疫抑制剂对Peycr斑的影响,Peycr斑通过肠道相关淋巴组织中肠道微生物和大分子的摄取和转运在粘膜免疫应答中发挥重要作用。已经表明FK和Cs可以产生免疫抑制作用,至少部分是通过减少颗粒的摄取和转运到派尔集合淋巴结中,以及通过减少派尔集合淋巴结FAE中免疫反应细胞的数量。免疫电镜观察发现,绒毛上皮标记细胞的微绒毛短于邻近的吸收细胞,线粒体稀疏,这是杯细胞的超微结构特征。波形蛋白丝被认为是在核周围的区域,并在杯细胞的质膜下延伸。在兔小肠中,波形蛋白可用于检测杯状细胞和M细胞,并可能有助于研究这些鲜为人知的细胞。少

项目成果

期刊论文数量(12)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Fujimura Y: "Evidence of M cells as portals of entry for antigens in the nasopharyngeal lymphoid tissue of humans"Virchows Archiv. 436. 560-566 (2000)
Fujimura Y:“M 细胞作为人类鼻咽淋巴组织中抗原进入门户的证据”Virchows Archiv。
  • DOI:
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    0
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  • 通讯作者:
Yoshinori Fujimura: "Evidence of M cells as portals of entry for antigens in the nasopharyngeal lymphoid tissue of human"Virchows Archiv. 436. 560-566 (2000)
Yoshinori Fujimura:“M 细胞作为人类鼻咽淋巴组织中抗原进入门户的证据”Virchows Archive。
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    0
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  • 通讯作者:
Fujimura Y, et al.: "Tacrolimus(FK506) and cyclosporine reduce the uptake and transport of particles into rabbit Peyer's patches"Transplantation. (in press).
Fujimura Y 等人:“他克莫司 (FK506) 和环孢菌素减少了颗粒对兔派尔氏淋巴结的吸收和转运”移植。
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  • 影响因子:
    0
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  • 通讯作者:
Yoshinori Fujimura Robert L.Owen: "The intestinal epithelial M cell -properties and function"In : Kirsner JB,ed.Inflammatory bowel disease W.B.Saunders Company : Philadelphia. 5th Edition. 33-44 (2000)
Yoshinori Fujimura Robert L.Owen:“肠上皮 M 细胞的特性和功能”,见:Kirsner JB,ed.炎症性肠病 W.B.Saunders 公司:费城。
  • DOI:
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  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Fujimura Y: "Evidence of M cells as potals of entry for antigens in the nasopharyngeal lymphoid tissue of humans"Virchows Archiv. 436. 560-566 (2000)
Fujimura Y:“M 细胞作为人类鼻咽淋巴组织中抗原进入点的证据”Virchows Archiv。
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    0
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FUJIMURA Yoshinori其他文献

FUJIMURA Yoshinori的其他文献

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{{ truncateString('FUJIMURA Yoshinori', 18)}}的其他基金

High-resolution analysis of bioresponse of functional food factor on the basis of metabolomics
基于代谢组学的功能性食品因子生物反应高分辨率分析
  • 批准号:
    24658124
  • 财政年份:
    2012
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Innovative multi-molecular imaging strategy toward comprehensive understanding of food factor signaling response contributed to the health promotion
创新的多分子成像策略全面了解食物因子信号反应有助于健康促进
  • 批准号:
    23680073
  • 财政年份:
    2011
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Young Scientists (A)
Development of highly precise mass spectrometric analytical system for creation of nutrimetabolomics
开发高精度质谱分析系统以创建营养代谢组学
  • 批准号:
    22658043
  • 财政年份:
    2010
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
The development of a vaccine delivery system through M-cells
通过 M 细胞开发疫苗输送系统
  • 批准号:
    15591072
  • 财政年份:
    2003
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似海外基金

Elucidation of molecular basis of GALT formation using novel Peyer's patch deficient mice
使用新型派尔氏集结缺陷小鼠阐明 GALT 形成的分子基础
  • 批准号:
    22H02822
  • 财政年份:
    2022
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Tuft cells and the regulation of Peyer's patch form and function
簇细胞和派尔氏淋巴集结形式和功能的调节
  • 批准号:
    565195-2021
  • 财政年份:
    2021
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Alexander Graham Bell Canada Graduate Scholarships - Master's
The role of Peyer's patch dendritic cells and macropahges in regulation of mucosal immune responses
派尔氏集结树突状细胞和巨噬细胞在调节粘膜免疫反应中的作用
  • 批准号:
    19K07631
  • 财政年份:
    2019
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Does IL-21 produced in Peyer's patch regulate the affinity of IgA and food allergy?
派尔氏淋巴集结中产生的 IL-21 是否调节 IgA 和食物过敏的亲和力?
  • 批准号:
    18K05478
  • 财政年份:
    2018
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
epidermal fatty acid binding protein(FABP) in Peyer's patch: a contribution to intesitnal flora control
派尔氏淋巴结中的表皮脂肪酸结合蛋白(FABP):对肠道菌群控制的贡献
  • 批准号:
    17K09368
  • 财政年份:
    2017
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Peyer's patch immune cells regulates intestinal bacteria: basic research for the manipulation of intestinal bacteria by foods
派尔氏集结免疫细胞调节肠道细菌:食物操纵肠道细菌的基础研究
  • 批准号:
    15K07438
  • 财政年份:
    2015
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
A novel pathway for small molecule delivery to Peyer's patch follicles
将小分子递送至派尔氏集结滤泡的新途径
  • 批准号:
    9222008
  • 财政年份:
    2015
  • 资助金额:
    $ 1.15万
  • 项目类别:
A novel pathway for small molecule delivery to Peyer's patch follicles
将小分子递送至派尔氏集结滤泡的新途径
  • 批准号:
    9094248
  • 财政年份:
    2015
  • 资助金额:
    $ 1.15万
  • 项目类别:
Association between intestinal inflammation and indigenous bacteria inhibit in Peyer's patch
肠道炎症与派尔氏淋巴集结中本地细菌抑制之间的关联
  • 批准号:
    26893136
  • 财政年份:
    2014
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Research Activity Start-up
Identification and functional analyses of the target genes of transcription factor Spi-B in Peyer's patch M cells
派尔氏淋巴集结M细胞转录因子Spi-B靶基因的鉴定及功能分析
  • 批准号:
    25860353
  • 财政年份:
    2013
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
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