Innovative multi-molecular imaging strategy toward comprehensive understanding of food factor signaling response contributed to the health promotion

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In this study, we attempted to understand the potential comprehensive relationship among a series of endogenous molecules with spatiotemporal information within tissue micro-regions for elucidating specific biological response to an orally dosed functional food factor. We succeeded in developing new techniques capable of two-dimensionally visualizing metabolome and proteome within tissue micro-regons, namely, multi-omics imaging, by improving a MALDI-MS technique. In addition, this analytical approach enabled us to simultaneously visualize orally dosed green tea catechin and metabolome within tissue micro-regions, and further visualized co-localization of green tea catechin and its target molecule. These results will provide a novel basic technology for understanding of precise food factor signaling response.