High-resolution analysis of bioresponse of functional food factor on the basis of metabolomics

点击翻译按钮获取中文摘要

In this study, we attempted to develop a technique and methodology, understanding of MALDI ionization rationale and synthesis of new matrix, for improvement of molecular coverage and detection sensitivity toward high-resolution metabolite analysis on the basis of MALDI-MS capable of sensitively detecting metabolites from trace amount of cells. We succeeded in newly proposing a methodology of chemometric prediction of MALDI ionization based on the quantitative structure-property relationship (QSPR) analysis and in newly generating highly sensitive matrix for metabolites. In addition, we found new molecular mechanism, mitochondrial regulation system, of oxidative stress, which is often observed in biological responses against food factors.