High-resolution analysis of bioresponse of functional food factor on the basis of metabolomics

基于代谢组学的功能性食品因子生物反应高分辨率分析

• 批准号: 24658124
• 负责人: FUJIMURA Yoshinori
• 金额: $ 2.33万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658124/
• 关键词: メタボロミクス; MALDI-MS; マトリックス; 質量分析イメージング; 代謝物プロファイリング; 機能性食品; 質量分析; 緑茶カテキン

In this study, we attempted to develop a technique and methodology, understanding of MALDI ionization rationale and synthesis of new matrix, for improvement of molecular coverage and detection sensitivity toward high-resolution metabolite analysis on the basis of MALDI-MS capable of sensitively detecting metabolites from trace amount of cells. We succeeded in newly proposing a methodology of chemometric prediction of MALDI ionization based on the quantitative structure-property relationship (QSPR) analysis and in newly generating highly sensitive matrix for metabolites. In addition, we found new molecular mechanism, mitochondrial regulation system, of oxidative stress, which is often observed in biological responses against food factors.

在这项研究中，我们试图开发一种技术和方法，了解MALDI电离原理和合成新的矩阵，以提高分子覆盖率和检测灵敏度，对高分辨率代谢物分析的基础上，MALDI-MS能够灵敏地检测微量细胞的代谢物。我们成功地在新提出的方法化学计量学预测的MALDI电离的基础上的定量结构-性质关系（QSPR）分析，并在新生成的高灵敏度的代谢物矩阵。此外，我们还发现了一种新的氧化应激的分子机制，即线粒体调控系统，这是在生物对食物因素的反应中经常观察到的。

项目成果

Statistical Investigation of Metabolite Structure to Reveal the Principle for Preferred Ionization in MALDI using 9-Aminoacridine

对代谢物结构进行统计研究，揭示使用 9-氨基吖啶在 MALDI 中优先电离的原理

• 发表时间: 2012
• 作者: Yukihira D;Fujimura Y;Shindo M;Miura D;and Wariishi H
• 通讯作者: and Wariishi H

Metabolomics-driven chemical sensing strategy for better understanding of functions of green tea

代谢组学驱动的化学传感策略可更好地了解绿茶的功能

• 发表时间: 2014
• 作者: Setoyama D;Fujimura Y;Miura D;藤村由紀;Fujimura Y
• 通讯作者: Fujimura Y

A trial for in situ label-free imaging of an orally dosed vegetable flavonoid within mammalian tissue

哺乳动物组织内口服蔬菜类黄酮原位无标记成像试验

• 发表时间: 2014
• 作者: Miura D;Fujimura Y;Nakamura A;Matsumoto Y;and Wariishi H
• 通讯作者: and Wariishi H

High-throughput analysis for metabolic dynamics and in situ metabolite imaging by MALDI mass spectrometry

通过 MALDI 质谱进行代谢动力学和原位代谢物成像的高通量分析

• 发表时间: 2012
• 作者: Miura D;Fujimura Y;Ymaguchi S;Ojima N;Shindo M;and Wariishi H
• 通讯作者: and Wariishi H

Quantitative Structure-Property Relationship of Metabolites to Ionization Efficiency Assisted by 9-Aminoacridine in MALDI

MALDI 中 9-氨基吖啶辅助代谢物与电离效率的定量构效关系

• 发表时间: 2012
• 作者: Yukihira D;Miura D;Fujimura Y;Kaku M;Tomonaga N;and Wariishi H
• 通讯作者: and Wariishi H