Development of highly precise mass spectrometric analytical system for creation of nutrimetabolomics

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Herein, we attempted to establish a novel evaluation method of function of fbod factor based on two-dimensional molecular behavior in local tissuemicro-regions, by developing an advanced MAI. DI-MS system capable of visualizing of bothexoge nous food factors and endogenous metabolites simultaneously. Because MS selecti vityand detection limit against various molecules are dependent on its ionization rate, we performed the screening of matrix, which can aid the ionization of both food factor and endogenous metabolites, and its optimization. The obtained matrix enabled us totwo-dimensionally visualize the green tea catechin EGCG in tissue micro-regions after theoral administration of EGCG and endogenous metabolites in response to EGCG. Theseresults suggest that our proposed MS system may contribute to the establishment of highlyprecise and reliable technique fbr evaluating the potential function of fbod factor in view ofefficacy and safbty.