Development of highly precise mass spectrometric analytical system for creation of nutrimetabolomics

开发高精度质谱分析系统以创建营养代谢组学

• 批准号: 22658043
• 负责人: FUJIMURA Yoshinori
• 金额: $ 1.92万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22658043/
• 关键词: 食品機能; 緑茶カテキン; ニュートリメタボロミクス; 代謝物; MALDI-MS; マトリックス; 質量分析イメージング; 生体局所応答; 質量分析; 機能性食品成分; ポリフェノール; MALDI

Herein, we attempted to establish a novel evaluation method of function of fbod factor based on two-dimensional molecular behavior in local tissuemicro-regions, by developing an advanced MAI. DI-MS system capable of visualizing of bothexoge nous food factors and endogenous metabolites simultaneously. Because MS selecti vityand detection limit against various molecules are dependent on its ionization rate, we performed the screening of matrix, which can aid the ionization of both food factor and endogenous metabolites, and its optimization. The obtained matrix enabled us totwo-dimensionally visualize the green tea catechin EGCG in tissue micro-regions after theoral administration of EGCG and endogenous metabolites in response to EGCG. Theseresults suggest that our proposed MS system may contribute to the establishment of highlyprecise and reliable technique fbr evaluating the potential function of fbod factor in view ofefficacy and safbty.

在此，我们试图建立一种基于局部组织微观区域二维分子行为的fbod因子功能评估新方法，通过开发先进的MAI。DI-MS系统能够同时显示外源性食物因子和内源性代谢产物。由于MS对各种分子的选择性和检出限取决于其电离速率，因此我们进行了有利于食物因子和内源性代谢物电离的基质筛选，并对其进行了优化。所获得的基质使我们能够二维可视化绿茶儿茶素EGCG在组织微区域口服EGCG和内源性代谢产物对EGCG的反应后。上述结果提示，从功效和安全性的角度出发，建立了一种高精度、可靠的fbod因子潜在功能评价方法。

项目成果

High-throughput Metabolic Profiling and in situ Metabolite Imaging by MALDI mass spectrometry

通过 MALDI 质谱进行高通量代谢分析和原位代谢物成像

• 发表时间: 2011
• 作者: Miura D;Yukihira D;Fujimura Y;Wariishi H
• 通讯作者: Wariishi H

TLR4 Signaling Inhibitory Pathway Induced by Green Tea Polyphenol Epigallocatechin-3-Gallate through 67-kDa Laminin Receptor

• DOI: 10.4049/jimmunol.0903742
• 发表时间: 2010-07-01
• 期刊: JOURNAL OF IMMUNOLOGY
• 影响因子: 4.4
• 作者: Byun, Eui Hong;Fujimura, Yoshinori;Tachibana, Hirofumi
• 通讯作者: Tachibana, Hirofumi

TLR4 signaling inhibitoxy pathway induced by green tea polyphenol epigallocatechin-3-gallate through 67-kDa laminin receptor. J

绿茶多酚表没食子儿茶素-3-没食子酸酯通过 67-kDa 层粘连蛋白受体诱导 TLR4 信号传导抑制途径。

• 发表时间: 2010
• 期刊: Immunol
• 作者: Byun EH Fujimura Y;Yamada K;and Tachibana H.
• 通讯作者: and Tachibana H.

生体低分子のMAHDI-MS測定に有効なマトリックスの開発

开发用于小生物分子 MAHDI-MS 测量的有效基质

• 发表时间: 2011
• 作者: 下農健治;堀川祥生;杉山淳司;今井友也;寺井康了,行平大地,三浦大典,藤村由紀,割石博之,新藤充
• 通讯作者: 寺井康了,行平大地,三浦大典,藤村由紀,割石博之,新藤充

生体低分子のMALDI-MS測定に有効なマトリックスの開発

开发用于小生物分子 MALDI-MS 测量的有效基质

• 发表时间: 2011
• 作者: 寺井康了;行平大地;三浦大典;藤村由紀;割石博之;新藤充
• 通讯作者: 新藤充