studies on Proliferation and Differenntiation of Fibroblast in Gastric Mucosa by HElicobacter pylori infection

幽门螺杆菌感染对胃粘膜成纤维细胞增殖和分化的研究

• 批准号: 12670539
• 负责人: NAKAMURA Masahiko
• 金额: $ 2.05万
• 依托单位: The Kitasato Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670539/
• 关键词: Helicobacter pylori; Fibroblast; Myofibrablast; Basic fibloblast Growth Factor; Plaelet-devived Growth Factor; Endothelin; Leptin; Leptin Receptor; ヘリコパクターピロリ; 筋線維芽細胞370; leptin receptor; cyclooxygenase II; transforming growth factorβ

To clcrnfy the mechanism of the myofibroblast proliferation, the binding sites of basic fibroblast growth factor (bFGF), plactietet-derived growth factor (PDGF), endothelin as well as their receptor, ware investingated by autoradiogrophy and in sitill hybridization As a result, the recepters of bFGF, PDGF-BB and Endothelin-1 were found to be distributed on the mesenchymal cells in chiding fibroblasts and myofibroblasts in the control group, while in the Helicobacter pytori-in fected Mongolian gerbils the number of CS23-bFGF, anacid-stable bFGF derivative, brought about the marlced increase of the number of myofibroblasts and FGF receptors. In addition, the localization of leptin and its receptor was investingated because leptin has recently atracted attention in relation to activation of fat-storing cells. In the gastric mucos of the control Mongolian gevbils, leptin immunoreactivity was observed in the endothelial cells of the microcirabatory units. In Helicobacter phylori-infected gerbits, teptin and its receptor ware recognized in the undifferentiated mesonohymal cells and myofibroblasts.

为探讨肌成纤维细胞增殖的机制，用放射自显影和原位杂交的方法，对碱性成纤维细胞生长因子(BFGF)、血小板衍生生长因子(PDGF)、内皮素及其受体的结合部位进行了研究。结果发现，对照组成纤维细胞和肌成纤维细胞间充质细胞上均有bFGF、PDGF-BB和Endothelin-1的受体分布，而感染的蒙古沙土鼠感染的嗜热螺杆菌中，CS23-bFGF和耐酸的衍生型bFGF引起成肌成纤维细胞和成纤维细胞的数量显著增加。此外，由于瘦素最近引起了与脂肪储存细胞激活有关的关注，因此对瘦素及其受体的定位进行了研究。在正常蒙古沙土鼠胃粘膜中，瘦素免疫反应位于微循环单位的内皮细胞中。在幽门螺杆菌感染的生殖细胞中，Teptin及其受体在未分化的中膜细胞和肌成纤维细胞中被识别。

项目成果

中村正彦: "胃粘膜における自律神経の意義-Helicobacter pylori感染と自律神経"自律神経. 38. 144-147 (2001)

中村正彦：“胃粘膜自主神经的意义 - 幽门螺杆菌感染和自主神经”自主神经。 38. 144-147 (2001)

Nakamura M. Akiba, Y., Yanaka, A., Ishii, H.: "Structure and function of esophageal cardial gland as a new effector sites of PPI."Digestion. 32. 355-359 (2001)

Nakamura M. Akiba, Y.、Yanaka, A.、Ishii, H.：“食管贲门腺作为 PPI 新效应位点的结构和功能。”消化。

Nakamura, M., Akiba, Y., Matsui, H., Ishii, H.: "Angiogenesis during heating of acetic acid-induced gastric aicer."Ulcer Research. 28. 5-7 (2001)

Nakamura, M.、Akiba, Y.、Matsui, H.、Ishii, H.：“乙酸诱导胃酸加热期间的血管生成”。溃疡研究。

中村正彦: "酢酸潰瘍再生過程における血管新生:塩基性線維芽細胞増殖因子と微小循環構築"Ulcer Research. 28・1. 5-7 (2001)

中村雅彦：“乙酸溃疡再生过程中的血管生成：碱性成纤维细胞生长因子和微循环结构”溃疡研究28・1（2001）。

中村正彦,岸川浩,土本寛二,石井裕正: "ラット胃底腺粘膜内における3H-cimetidine結合部位の再評価。"Ther.Res.. 21・Sup1. S49-S56 (2000)

Masahiko Nakamura、Hiroshi Kishikawa、Kanji Tsuchimoto、Hiromasa Ishii：“大鼠胃底腺粘膜中 3H-西咪替丁结合位点的重新评估。”21・Sup1（2000）。