Identification and characterization of follicular stem cells

卵泡干细胞的鉴定和表征

• 批准号: 12670825
• 负责人: NARISAWA Yutaka
• 金额: $ 2.11万
• 依托单位: Saga Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670825/
• 关键词: Merkel cell; Langerhans cell; hair follicle; three-dimension; 3次元; 2次元; 毛嚢; 幹細胞; メンケル細胞; 毛盤; メラノサイト; β-lintegrin; E-cadherin; α-catenin; γ-catenin; EGF receptor

The bulge area has not been paid attention except for the knowledge that the bulge area is the attachment site of the arrector pili muscles. However, it was suggested that follicular stem cells reside within the bulge area of human hair follicles, Therefore, we focused the characterization of epithelial cells composed of the bulge area. Immunohistochemical staining was carried out on the EDTA treated extracted human hair follicles, which enabled us to evaluate the true localization of each antibody labeled cells in three dimensions. NKI/beteb reactive melanocytes, CDla reactive Langerhans cells, cytokeratin!9 and/or cytokeratin15 reactive pleuripotential amplifying cells and cytokeratin20 reactive Merkel cells were clearly shown within the bulge. These findings of the extracted hair follicles were confirmed by the conventional vertical sections. Interestingly, double immunostaining techniques demonstrated that ir-Merkel cells contacted with ir-Langerhans cells within the bulge area. Theses findings may indicate some functional relationship between Merkel cells and Langerhans cells.

除了知道隆起区是立毛肌皮利的附着部位外，隆起区一直没有受到重视。然而，有人认为毛囊干细胞存在于人毛囊的隆突区，因此，我们将重点放在隆突区组成的上皮细胞的特性。对EDTA处理的提取的人毛囊进行免疫组织化学染色，这使我们能够在三维中评估每个抗体标记的细胞的真实定位。NKI/beteb反应性黑素细胞、CD 1a反应性朗格汉斯细胞、细胞角蛋白！9和/或细胞角蛋白15反应性胸膜放大细胞和细胞角蛋白20反应性默克尔细胞在隆起内清楚地显示。常规垂直切片证实了毛囊的这些发现。有趣的是，双重免疫染色技术表明，在隆突区域内，ir-Merkel细胞与ir-Langerhans细胞接触。提示默克尔细胞和朗格汉斯细胞之间存在一定的功能联系。

项目成果

Tatsurou Tanaka, Yutaka Narisawa: "Three-dimensional comparative study of Merkel cell distribution in various body sites of guinea pigs and rats"Arch Dermatol Res. 292. 173-179 (2000)

Tatsurou Tanaka、Yutaka Narisawa：“豚鼠和大鼠不同身体部位默克尔细胞分布的三维比较研究”Arch Dermatol Res。