Identification and differentiation of Merkel stem cells or precursor cells

Merkel干细胞或前体细胞的鉴定和分化

• 批准号: 18591252
• 负责人: NARISAWA Yutaka
• 金额: $ 2.49万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591252/
• 关键词: Merkel cell; stem cell; precursor cell; コラーゲンゲル培養法; 表皮シート; 培養; 気相-液相界面微小環境; ケラチノサイト

Merkel cells exist in the basal layer of the epidermis, more especially at the bottom of the rete ridges, contacting with keratinocytes. We thus hypothesized that a direct contact between Merkel cells and keratinocytes may be relevant to the maintenance of Merkel cell survival and function. To address our hypothesis, we developed a new organotypic culture system of Merkel cells, using Merkel cell-containing epidermal sheets. This culture system maintains Merkel cell survival within epidermal rete ridges for more than 2 weeks without neurotrophic growth factors (neurotrophin-3 and nerve growth factor). Coculture of Merkel cells with nerve cells (PC12) promoted Merkel cell survival within epidermal sheets, in which keratinocytes replicated cytokeratins (CK1, CK10, CK14) and p63 expression of in vivo keratinocytes. Merkel cell proliferation was not detected in all conditions. The data suggest, firstly, that a direct contact between Merkel cells and keratinocytes is one of the critical factors in Merkel cell survival ; secondly that Merkel cells may be a terminally differentiated cell type. Our culture system will open up a new way to study Merkel cell biology.

默克尔细胞存在于表皮的基底层，尤其是在网状脊的底部，与角质形成细胞接触。因此，我们假设默克尔细胞和角质形成细胞之间的直接接触可能与默克尔细胞存活和功能的维持有关。为了解决我们的假设，我们使用含有默克尔细胞的表皮片开发了一种新的默克尔细胞器官型培养系统。该培养系统无需神经营养生长因子（神经营养蛋白-3 和神经生长因子）即可在表皮网脊内维持 Merkel 细胞存活 2 周以上。 Merkel 细胞与神经细胞 (PC12) 共培养促进 Merkel 细胞在表皮片内存活，其中角质形成细胞复制体内角质形成细胞的细胞角蛋白（CK1、CK10、CK14）和 p63 表达。并未在所有条件下检测到默克尔细胞增殖。数据表明，首先，默克尔细胞与角质形成细胞的直接接触是默克尔细胞存活的关键因素之一；其次，默克尔细胞可能是一种终末分化的细胞类型。我们的培养系统将为研究默克尔细胞生物学开辟一条新途径。

项目成果

メルケル細胞の表皮シートを用いた新規培養法の確立

默克尔细胞表皮片新培养方法的建立

• 发表时间: 2007
• 作者: 永瀬 浩太郎;成澤 寛;内橋 和芳;青木 茂久;戸田 修二
• 通讯作者: 戸田 修二

Merkel cell-poor trichoblastoma with basal cell carcinoma-like foci

默克尔细胞贫型毛母细胞瘤伴基底细胞癌样病灶

• 发表时间: 2007
• 期刊: Am J Dermatopathol 29
• 作者: Misago N;Satoh T;Miura Y;Nagase K;Narisawa Y.
• 通讯作者: Narisawa Y.

Establishment of a new culture system of Merkel cells using epidermal sheett

利用表皮片建立默克尔细胞新培养体系

• 发表时间: 2007
• 作者: Nagase;K.;Narisawa;Y.;Uchihashi;K.;Aoki;S.;Toda;S
• 通讯作者: S