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Identification and characterization of follicular stem cells

卵泡干细胞的鉴定和表征

基本信息

批准号：
09670884
负责人：
NARISAWA Yutaka
金额：
$ 2.11万
依托单位：
Saga Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670884/
关键词：
Merkel cell Langerhans cell hair follicle three-dimension 3次元 2次元 Merkel cell hair follicle rat mouse アポトーシス外毛根鞘最内層細胞 TUNEL法 apoptitic pocket like structure 毛隆起

项目摘要

The presence of Merkel cells and Langerhans cells were reported within the hair follicles on the light microscope and electron microscope. However, nobody has ever paid attention to the relationship between Merkel cells and Langerhans cells. We carried out double immunostaining techniques for Merkel cells and Langerhans cells using serial conventional paraffinized transverse sections of human scalp skin. CAM5.2 reactive Merkel cells and S-100 protein reactive Langerhans cells were clearly identified. Consequently, in some case cell bodies of Merkel cells and Langerhans cells were closely contacted with each other. Moreover, a few cases showed the direct connections with dendritic processes of Merkel cells and Langerhans cells.We prepared isolated human vellus hair follicles with EDTA. The morphology and antigenecity of these samples was well preserved, which enabled us to investigate the three-dimensional relationship between Merkel cells and Langerhans cells of human hair follicles. Merkel cells were stained with CK2O and Langerhans cells with CD1a, respectively. The three-dimensional views showed the direct contact with dendritic processes of immunoreactive Merkel cells and Langerhans cells. These findings may indicate some functional relationship between Merkel cells and Langerhans cells.

在光学显微镜和电子显微镜下，毛囊内存在默克尔细胞和朗格汉斯细胞。然而，从未有人关注过默克尔细胞和朗格汉斯细胞之间的关系。我们使用人类头皮皮肤的连续常规石蜡横切片对默克尔细胞和朗格汉斯细胞进行了双重免疫染色技术。 CAM5.2 反应性 Merkel 细胞和 S-100 蛋白反应性 Langerhans 细胞被清楚地识别出来。因此，有时默克尔细胞和朗格汉斯细胞的细胞体彼此紧密接触。此外，少数病例显示与默克尔细胞和朗格汉斯细胞的树突状突起有直接联系。我们用EDTA制备了分离的人毳毛毛囊。这些样本的形态和抗原性保存完好，使我们能够研究人毛囊默克尔细胞和朗格汉斯细胞之间的三维关系。默克尔细胞分别用 CK2O 染色，朗格汉斯细胞用 CD1a 染色。三维视图显示与免疫反应默克尔细胞和朗格汉斯细胞的树突状突起的直接接触。这些发现可能表明默克尔细胞和朗格汉斯细胞之间的某些功能关系。